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The map label for this gene is prfB

Identifier: 116329128

GI number: 116329128

Start: 3024925

End: 3026028

Strand: Direct

Name: prfB

Synonym: LBL_2551

Alternate gene names: 116329128

Gene position: 3024925-3026028 (Clockwise)

Preceding gene: 116329127

Following gene: 116329129

Centisome position: 83.69

GC content: 41.58

Gene sequence:

>1104_bases
ATGGAAGTCAAATCAGCAAAAGAACTTAAAAGAGTCTCCAAAGAATTACAGGAAAATTTTCTAAATCGTTGGAAACTCCT
AAATTTGGAACAGGACAAGGATCGCCTTAAGGCACTCAATGAAAAGTCTGAAGATCCGGACCTGTGGAACAATCCGGAAG
AAGCGAGAATTGTGAGTCAGAAAAAGAACGAACTGGAAAAAAAACTCACTCCTTGGTTTACGATTCAACAAGATATATTA
GATTTTCCTGATTTAGTAGAGTTAACTCTCGACGAAAAAGGCGAAAACGGAATCGGAGAGCTTTCGATGGAATACAACAG
GCTTCAGGAAAAATTCGAAGAACTGGAACTTTTGGGCGCTCTTAAAAATTCGGAGGATCTTAAACCGGCTTTCCTTAACA
TTCATCCCGGAGCGGGTGGAACCGAAAGTCAAGACTGGGCTGAAATGCTTCTTAGGATGTACATTCGATATTTTGAAAAA
AAGGGATATCAGTATTCTCTCATTGATATTCAGGCGGGCGACGGAGCCGGAATCAAAAATGTCACCTTACACGTAGTAGG
TGATTTCGCTTTCGGTTTTTTAAAAGGGGAAAACGGAATTCACAGACTCGTAAGGATTTCTCCTTTTGATGCAAACAAAA
GGAGGCATACCTCATTCGTTTCAGTCCACGTAAGCCCCGAGATAGACGACGAAATCGATATTAAAATCGAGGAAAAAGAC
ATTCGTGTGGATGTATATCGTTCTTCGGGAGCCGGTGGACAACACGTCAACACAACCGATTCCGCAGTTAGAATCACTCA
TCTTCCTAGCGGAATCGTCGTCGCTTGCCAAAACGAGAGATCTCAGATCAAAAACAGGGATACCGCATTTAAAATGTTAA
AGGCAAGACTCTACGAGATGGAACAGGAAAAAGCCAAGGAAGAATTGGAAAAAAAATCCGGCGAAAAAAAAGACATCGCT
TGGGGTTCTCAGATTCGAAGTTACGTCTTCCATCCTTACAATCTTGTGAAAGACCATAGAACTGATCATGAAACCGGAAA
CGTCGCGGCAGTGATGGACGGAGATATAGAACCGTTCATTCTTGCATATTTAAAAACGCTTTAA

Upstream 100 bases:

>100_bases
GAAAGAGAAAATAAAGAAGATTTTCTTTTTCAATAAATGGCTCCTCTCAAACCATCTTACAGCAAATTACAAATTATTAA
ATCAACCGCAAACAAAGATT

Downstream 100 bases:

>100_bases
GAAAAAAGTGCAGGCATCGATGATAGATGCGTGTTTTTACTCTAACACAATACAATAGAGTTGTTGAAGAATTAATTCTC
TATCCGTTTCTGCTGCATTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEVKSAKELKRVSKELQENFLNRWKLLNLEQDKDRLKALNEKSEDPDLWNNPEEARIVSQKKNELEKKLTPWFTIQQDIL
DFPDLVELTLDEKGENGIGELSMEYNRLQEKFEELELLGALKNSEDLKPAFLNIHPGAGGTESQDWAEMLLRMYIRYFEK
KGYQYSLIDIQAGDGAGIKNVTLHVVGDFAFGFLKGENGIHRLVRISPFDANKRRHTSFVSVHVSPEIDDEIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHLPSGIVVACQNERSQIKNRDTAFKMLKARLYEMEQEKAKEELEKKSGEKKDIA
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL

Sequences:

>Translated_367_residues
MEVKSAKELKRVSKELQENFLNRWKLLNLEQDKDRLKALNEKSEDPDLWNNPEEARIVSQKKNELEKKLTPWFTIQQDIL
DFPDLVELTLDEKGENGIGELSMEYNRLQEKFEELELLGALKNSEDLKPAFLNIHPGAGGTESQDWAEMLLRMYIRYFEK
KGYQYSLIDIQAGDGAGIKNVTLHVVGDFAFGFLKGENGIHRLVRISPFDANKRRHTSFVSVHVSPEIDDEIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHLPSGIVVACQNERSQIKNRDTAFKMLKARLYEMEQEKAKEELEKKSGEKKDIA
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL
>Mature_367_residues
MEVKSAKELKRVSKELQENFLNRWKLLNLEQDKDRLKALNEKSEDPDLWNNPEEARIVSQKKNELEKKLTPWFTIQQDIL
DFPDLVELTLDEKGENGIGELSMEYNRLQEKFEELELLGALKNSEDLKPAFLNIHPGAGGTESQDWAEMLLRMYIRYFEK
KGYQYSLIDIQAGDGAGIKNVTLHVVGDFAFGFLKGENGIHRLVRISPFDANKRRHTSFVSVHVSPEIDDEIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHLPSGIVVACQNERSQIKNRDTAFKMLKARLYEMEQEKAKEELEKKSGEKKDIA
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI34577120, Length=311, Percent_Identity=35.3697749196142, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI166795303, Length=230, Percent_Identity=38.695652173913, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI166795305, Length=140, Percent_Identity=37.1428571428571, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=47.6470588235294, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=35.7954545454545, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=243, Percent_Identity=31.2757201646091, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=237, Percent_Identity=39.662447257384, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19921226, Length=229, Percent_Identity=40.174672489083, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_LEPBJ (Q04V47)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799981.1
- ProteinModelPortal:   Q04V47
- SMR:   Q04V47
- STRING:   Q04V47
- GeneID:   4409427
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0528
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   Q04V47
- ProtClustDB:   PRK00578
- BioCyc:   LBOR355277:LBJ_0528-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 42150; Mature: 42150

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKSAKELKRVSKELQENFLNRWKLLNLEQDKDRLKALNEKSEDPDLWNNPEEARIVSQ
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KKNELEKKLTPWFTIQQDILDFPDLVELTLDEKGENGIGELSMEYNRLQEKFEELELLGA
HHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKNSEDLKPAFLNIHPGAGGTESQDWAEMLLRMYIRYFEKKGYQYSLIDIQAGDGAGIKN
HCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCE
VTLHVVGDFAFGFLKGENGIHRLVRISPFDANKRRHTSFVSVHVSPEIDDEIDIKIEEKD
EEEEEEECHHEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCE
IRVDVYRSSGAGGQHVNTTDSAVRITHLPSGIVVACQNERSQIKNRDTAFKMLKARLYEM
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHCHHHHHHHHHHHHHHH
EQEKAKEELEKKSGEKKDIAWGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCHHH
LAYLKTL
HHHHHHC
>Mature Secondary Structure
MEVKSAKELKRVSKELQENFLNRWKLLNLEQDKDRLKALNEKSEDPDLWNNPEEARIVSQ
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KKNELEKKLTPWFTIQQDILDFPDLVELTLDEKGENGIGELSMEYNRLQEKFEELELLGA
HHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKNSEDLKPAFLNIHPGAGGTESQDWAEMLLRMYIRYFEKKGYQYSLIDIQAGDGAGIKN
HCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCE
VTLHVVGDFAFGFLKGENGIHRLVRISPFDANKRRHTSFVSVHVSPEIDDEIDIKIEEKD
EEEEEEECHHEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCE
IRVDVYRSSGAGGQHVNTTDSAVRITHLPSGIVVACQNERSQIKNRDTAFKMLKARLYEM
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHCHHHHHHHHHHHHHHH
EQEKAKEELEKKSGEKKDIAWGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCHHH
LAYLKTL
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA