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The map label for this gene is kinA [H]

Identifier: 116329064

GI number: 116329064

Start: 2931184

End: 2932695

Strand: Direct

Name: kinA [H]

Synonym: LBL_2475

Alternate gene names: 116329064

Gene position: 2931184-2932695 (Clockwise)

Preceding gene: 116329059

Following gene: 116329065

Centisome position: 81.1

GC content: 42.26

Gene sequence:

>1512_bases
ATGGAATCAAAGTCTACAACAGTGCTCGTTATCGACGACGAATTCGAAATTCGTACGGTTTTAGAAAGGGTTATTTCCAG
AGAAGGGTATCGAGTATTATTGGCGAAGGATTTCGATTCTGCTCTTGAAATTATCCGAAGTCAAAAAATTGACGTGGTAA
TTTCGGATATCGTTATGAACGGTAAAAACGGCATCGAAGTCACGAAAGAAGTTCGCAAAATCAACGAGAACATTCCGGTC
GTCTTAATGACCGGAAATCCTGATCTTGCCACCGCAGAAGAAGCCGTCCGTAACCGCGTGTTCGATTATATTTCCAAACC
GATCCGTCGCACGAGTATTTTGGAAGTTTTCAAAAAAGCTACAATAGAAAAGGAAATTCGGGATCAACACACGGAAACCC
TGATCAGATCCGAGACTGAAAATACTAAACTCGCACAAAGAGCCAAGGATTTATATTTACAAAATTATAATATTCTAAAC
GCAACGAGCGATTGTGTGATCACGTTGGATCGGGATTTGAGATTCTCCGGTTTAAACCAATCCGCTTTGGACGCGTTCGG
ATATACCGAAGAGGAAATTCTCGGAAAACATTTCAACATACTGATCCCCCCGGAAAAAGAAAAACTCTATATGGAAAAAG
TGGCTCTGCTTTTAAAAAGAAAAAATAAGAGACAAATCGCCAGAATTAGCCGTTCCGACTTGATGAGCAGGGACGGAGAG
GTGAGAACCTACGATATTTCCGTTTGTTATTACGACATCGAAGGCCAGACGTATTATACCGGAATTGCAAGGGATATCAC
GAATCAACTTTTGATTTCGGAAAAACTCATAGACGCGGAGCGGCGCGCGTTTCTTTCCGTTCTCGCTTCAAGCATCGGAC
ACGAGATCAACAACTCATTAACTGCGATTCAGGGACATATCGAAATCGCGAAACTTCCCGACGCCACGGAACAGATCCGC
CAAAAGACGATCCAAATTACCTGGGGCCAATTGATCAAGTTGCAAACCCTCACGAACAACCTTTTACAATTGGGAAAACC
CGGAGAAAGTTTGAGTAAATCTTTGGAATCGATCCATCTGAATGATTCTGTGGAAAGTGTGATCGACGTTTTTCAAAAAA
CTTCCAGACTTAAAGACTGTAGGATCTATTTTAAACCCGGACCGGCTTCGGTAATCGTGAAGTCGGATCAGGACCAGCTT
TCCCTCTTACTTTCCAACATCGTTTTAAACTCCGCGGACGCGACCGGCAATCGAGGAAATATTCAAATTTCCGTATATAT
TAGAAATCATCACCCGGTCGTCTCCGTTTTGGACGACGGTGTTGGAATGAGTAAAGAAGTGATTCAAAAAATTTATCAAC
CTTATTTTACGACAAAAGGAATCGGAAAAGGAACAGGACTTGGAATGTTCGTTGCCAAAGAGATAGCGGATCAGCGTGGA
ATCAAAATCGAAATCGAATCGGAACCGAATTTAGGAACGGAATTTCGTTTGGTTTTTCCGGATAAGGTATAA

Upstream 100 bases:

>100_bases
TTTGGAATCTTTGCCCGGAAACGGAACGAGGGTTTTGATTCGCCTTCCTGTTTAGGACGTTTTTTTTGGTAAAATAAGGT
TTTTCCCAGGCTTAAAAGTC

Downstream 100 bases:

>100_bases
ATGATATGAATACTTCCGTCTCTTTAACGGATCAAGAATTGAAGTTTCTGGATTTCTCCGGAAAACATCCTCAAAAAATT
TATCCGATCACCTCGGAAGC

Product: sensor histidine kinase and response regulator of a two component complex

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MESKSTTVLVIDDEFEIRTVLERVISREGYRVLLAKDFDSALEIIRSQKIDVVISDIVMNGKNGIEVTKEVRKINENIPV
VLMTGNPDLATAEEAVRNRVFDYISKPIRRTSILEVFKKATIEKEIRDQHTETLIRSETENTKLAQRAKDLYLQNYNILN
ATSDCVITLDRDLRFSGLNQSALDAFGYTEEEILGKHFNILIPPEKEKLYMEKVALLLKRKNKRQIARISRSDLMSRDGE
VRTYDISVCYYDIEGQTYYTGIARDITNQLLISEKLIDAERRAFLSVLASSIGHEINNSLTAIQGHIEIAKLPDATEQIR
QKTIQITWGQLIKLQTLTNNLLQLGKPGESLSKSLESIHLNDSVESVIDVFQKTSRLKDCRIYFKPGPASVIVKSDQDQL
SLLLSNIVLNSADATGNRGNIQISVYIRNHHPVVSVLDDGVGMSKEVIQKIYQPYFTTKGIGKGTGLGMFVAKEIADQRG
IKIEIESEPNLGTEFRLVFPDKV

Sequences:

>Translated_503_residues
MESKSTTVLVIDDEFEIRTVLERVISREGYRVLLAKDFDSALEIIRSQKIDVVISDIVMNGKNGIEVTKEVRKINENIPV
VLMTGNPDLATAEEAVRNRVFDYISKPIRRTSILEVFKKATIEKEIRDQHTETLIRSETENTKLAQRAKDLYLQNYNILN
ATSDCVITLDRDLRFSGLNQSALDAFGYTEEEILGKHFNILIPPEKEKLYMEKVALLLKRKNKRQIARISRSDLMSRDGE
VRTYDISVCYYDIEGQTYYTGIARDITNQLLISEKLIDAERRAFLSVLASSIGHEINNSLTAIQGHIEIAKLPDATEQIR
QKTIQITWGQLIKLQTLTNNLLQLGKPGESLSKSLESIHLNDSVESVIDVFQKTSRLKDCRIYFKPGPASVIVKSDQDQL
SLLLSNIVLNSADATGNRGNIQISVYIRNHHPVVSVLDDGVGMSKEVIQKIYQPYFTTKGIGKGTGLGMFVAKEIADQRG
IKIEIESEPNLGTEFRLVFPDKV
>Mature_503_residues
MESKSTTVLVIDDEFEIRTVLERVISREGYRVLLAKDFDSALEIIRSQKIDVVISDIVMNGKNGIEVTKEVRKINENIPV
VLMTGNPDLATAEEAVRNRVFDYISKPIRRTSILEVFKKATIEKEIRDQHTETLIRSETENTKLAQRAKDLYLQNYNILN
ATSDCVITLDRDLRFSGLNQSALDAFGYTEEEILGKHFNILIPPEKEKLYMEKVALLLKRKNKRQIARISRSDLMSRDGE
VRTYDISVCYYDIEGQTYYTGIARDITNQLLISEKLIDAERRAFLSVLASSIGHEINNSLTAIQGHIEIAKLPDATEQIR
QKTIQITWGQLIKLQTLTNNLLQLGKPGESLSKSLESIHLNDSVESVIDVFQKTSRLKDCRIYFKPGPASVIVKSDQDQL
SLLLSNIVLNSADATGNRGNIQISVYIRNHHPVVSVLDDGVGMSKEVIQKIYQPYFTTKGIGKGTGLGMFVAKEIADQRG
IKIEIESEPNLGTEFRLVFPDKV

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=26.0869565217391, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=26.7782426778243, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790437, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=101, Percent_Identity=35.6435643564356, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=33.0708661417323, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788905, Length=120, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786600, Length=251, Percent_Identity=23.5059760956175, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=380, Percent_Identity=23.6842105263158, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=25.5905511811024, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=24.1803278688525, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56806; Mature: 56806

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKSTTVLVIDDEFEIRTVLERVISREGYRVLLAKDFDSALEIIRSQKIDVVISDIVMN
CCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEHHCC
GKNGIEVTKEVRKINENIPVVLMTGNPDLATAEEAVRNRVFDYISKPIRRTSILEVFKKA
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIEKEIRDQHTETLIRSETENTKLAQRAKDLYLQNYNILNATSDCVITLDRDLRFSGLNQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCEECCCCH
SALDAFGYTEEEILGKHFNILIPPEKEKLYMEKVALLLKRKNKRQIARISRSDLMSRDGE
HHHHHCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
VRTYDISVCYYDIEGQTYYTGIARDITNQLLISEKLIDAERRAFLSVLASSIGHEINNSL
EEEEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAIQGHIEIAKLPDATEQIRQKTIQITWGQLIKLQTLTNNLLQLGKPGESLSKSLESIHL
EEEECCEEEEECCCHHHHHHHHEEEEEHHHHEEHHHHHHHHHHCCCCCHHHHHHHHHEEC
NDSVESVIDVFQKTSRLKDCRIYFKPGPASVIVKSDQDQLSLLLSNIVLNSADATGNRGN
CCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCE
IQISVYIRNHHPVVSVLDDGVGMSKEVIQKIYQPYFTTKGIGKGTGLGMFVAKEIADQRG
EEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
IKIEIESEPNLGTEFRLVFPDKV
CEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MESKSTTVLVIDDEFEIRTVLERVISREGYRVLLAKDFDSALEIIRSQKIDVVISDIVMN
CCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEHHCC
GKNGIEVTKEVRKINENIPVVLMTGNPDLATAEEAVRNRVFDYISKPIRRTSILEVFKKA
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIEKEIRDQHTETLIRSETENTKLAQRAKDLYLQNYNILNATSDCVITLDRDLRFSGLNQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCEECCCCH
SALDAFGYTEEEILGKHFNILIPPEKEKLYMEKVALLLKRKNKRQIARISRSDLMSRDGE
HHHHHCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
VRTYDISVCYYDIEGQTYYTGIARDITNQLLISEKLIDAERRAFLSVLASSIGHEINNSL
EEEEEEEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAIQGHIEIAKLPDATEQIRQKTIQITWGQLIKLQTLTNNLLQLGKPGESLSKSLESIHL
EEEECCEEEEECCCHHHHHHHHEEEEEHHHHEEHHHHHHHHHHCCCCCHHHHHHHHHEEC
NDSVESVIDVFQKTSRLKDCRIYFKPGPASVIVKSDQDQLSLLLSNIVLNSADATGNRGN
CCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCE
IQISVYIRNHHPVVSVLDDGVGMSKEVIQKIYQPYFTTKGIGKGTGLGMFVAKEIADQRG
EEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCC
IKIEIESEPNLGTEFRLVFPDKV
CEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]