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The map label for this gene is alkA [H]

Identifier: 116329013

GI number: 116329013

Start: 2865278

End: 2865964

Strand: Direct

Name: alkA [H]

Synonym: LBL_2417

Alternate gene names: 116329013

Gene position: 2865278-2865964 (Clockwise)

Preceding gene: 116329012

Following gene: 116329014

Centisome position: 79.27

GC content: 40.03

Gene sequence:

>687_bases
ATGCCTCTTCCAGATTCTTTCCGACTTCCTAATCCTAAAAATCGTTCTTCTTTCGTTTTAGAAGATAGGAAGATTCGTCT
CCAAAAAGCATCCGATTGGCTTCGTAAAAAAGATCCAATCACTAAAAAACTGGTCGATTCCATCGGACCTTGTAAATTAC
AAACCATAGGGAACCCTTATCAGGTTTTGATTAAATCCGTTCTGGGTCAACAACTCTCCGTTAAAGTCGCCCTAACCTTC
GAACGTAGGTTGATTTCTCTCGCAGGAAGTAAAAAAATTCCTCCCCCGGATCGGATTCTAATGATTCCAAATGAGGAATT
GAAAAAGATAGGAGTTTCCCAAGCAAAGATTGAAACAATCCAACGAATCGCCGAAGCCTATTTGAATCGGGATATCACCG
ATTCAAAACTTCGTAAATTAGAAGATTCTGATGTTTTAAACCTTCTTTGCTCTTTTAAGGGCGTGGGGCCTTGGACGGCC
GAAATGGTTTTGATCTTCGCTTTGGATCGTTGGGATCATTTTTCCATCAACGATCTGATTCTTAGAAAATCGGTGGAAAA
ACACTATGGAATTTCCAAGGATAATAAAAAAGAAATCCAGCATTTTTTAATGAGTTATTCTCCCTTTAGAACGATCCTTT
CCTGGTATCTTTGGGCGGACATGGACGGTGGAGAAGGTTGGAACTAA

Upstream 100 bases:

>100_bases
GATTTAGTAGAACTGGAAAAGTTAGTTCATATTTATAGAAAAAAATCCCAAATGGAAAACAAACTCAAAAAAAGAGTATA
ATCCGTGTTTTCAAATTTGA

Downstream 100 bases:

>100_bases
GAAGGAACTTGTTCCAATACCCTTGAAGAAAAATCATCATTAGAGTTGTTGAAAAATTCCATGATTCGGATTAACAAAAC
CGCTTCAAACGTCCATTTCA

Product: DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MPLPDSFRLPNPKNRSSFVLEDRKIRLQKASDWLRKKDPITKKLVDSIGPCKLQTIGNPYQVLIKSVLGQQLSVKVALTF
ERRLISLAGSKKIPPPDRILMIPNEELKKIGVSQAKIETIQRIAEAYLNRDITDSKLRKLEDSDVLNLLCSFKGVGPWTA
EMVLIFALDRWDHFSINDLILRKSVEKHYGISKDNKKEIQHFLMSYSPFRTILSWYLWADMDGGEGWN

Sequences:

>Translated_228_residues
MPLPDSFRLPNPKNRSSFVLEDRKIRLQKASDWLRKKDPITKKLVDSIGPCKLQTIGNPYQVLIKSVLGQQLSVKVALTF
ERRLISLAGSKKIPPPDRILMIPNEELKKIGVSQAKIETIQRIAEAYLNRDITDSKLRKLEDSDVLNLLCSFKGVGPWTA
EMVLIFALDRWDHFSINDLILRKSVEKHYGISKDNKKEIQHFLMSYSPFRTILSWYLWADMDGGEGWN
>Mature_227_residues
PLPDSFRLPNPKNRSSFVLEDRKIRLQKASDWLRKKDPITKKLVDSIGPCKLQTIGNPYQVLIKSVLGQQLSVKVALTFE
RRLISLAGSKKIPPPDRILMIPNEELKKIGVSQAKIETIQRIAEAYLNRDITDSKLRKLEDSDVLNLLCSFKGVGPWTAE
MVLIFALDRWDHFSINDLILRKSVEKHYGISKDNKKEIQHFLMSYSPFRTILSWYLWADMDGGEGWN

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 26265; Mature: 26134

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPDSFRLPNPKNRSSFVLEDRKIRLQKASDWLRKKDPITKKLVDSIGPCKLQTIGNPY
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCHH
QVLIKSVLGQQLSVKVALTFERRLISLAGSKKIPPPDRILMIPNEELKKIGVSQAKIETI
HHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCCHHHHHHH
QRIAEAYLNRDITDSKLRKLEDSDVLNLLCSFKGVGPWTAEMVLIFALDRWDHFSINDLI
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
LRKSVEKHYGISKDNKKEIQHFLMSYSPFRTILSWYLWADMDGGEGWN
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLPDSFRLPNPKNRSSFVLEDRKIRLQKASDWLRKKDPITKKLVDSIGPCKLQTIGNPY
CCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCHH
QVLIKSVLGQQLSVKVALTFERRLISLAGSKKIPPPDRILMIPNEELKKIGVSQAKIETI
HHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCCHHHHHHH
QRIAEAYLNRDITDSKLRKLEDSDVLNLLCSFKGVGPWTAEMVLIFALDRWDHFSINDLI
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
LRKSVEKHYGISKDNKKEIQHFLMSYSPFRTILSWYLWADMDGGEGWN
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]