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The map label for this gene is rho [H]

Identifier: 116328952

GI number: 116328952

Start: 2789422

End: 2790867

Strand: Direct

Name: rho [H]

Synonym: LBL_2351

Alternate gene names: 116328952

Gene position: 2789422-2790867 (Clockwise)

Preceding gene: 116328951

Following gene: 116328953

Centisome position: 77.17

GC content: 46.27

Gene sequence:

>1446_bases
ATGGCAACAGTAAGACGAGACAATAAAAACAAACACCACAACCAAAATAATAACTACAGTCAAAACGAAACCACCGGTTC
TATCGAAGAAGAAAGCGCCGGACAGGAATCCCAAGACCTTGAATCTTCCGATAACGCGGATCATAGATCCCGTAAACGCA
AGCGAGGCGGCTACGAAGGTCCAACTCCGGCTCCCATCGATCTTGTAGAACTCAAGAAAAAAGCGATCGCCGACTTAATT
GAAGCTGCTAAAGGTCTCGGGGTCGAGAACACGAGCGGTCTCAAAAAACAGAACTTGATCTTTGCCATTCTACAAGCTCA
GGCGGAAAGAGACGGCCAAGTACACGCCGCTGGTGTGATGGAAAAACTTCCGGACGGTTACGGTTTTTTAAGATCTCCCG
ACTATAACTATGTTCCTGGTCCGGATGATATTTATGTTTCTCCTTCTCAAATTAAACTCTTCGGTTTAAGAACCGGGGAC
ACAGTAGAAGGACAAATCCGTCCTCCGAAAGAATCCGAAAGATTCTTTGCGATGCTCCGTGTGGAAACCGTCAACGGTTA
TACTCCCGACGTCGCAGGTAAACGTGCTCTCTTCGATAATTTGACTCCTTTATATCCGAATGAAAGGCTCAAGATGGAAT
ACGATCCTTCCATGCTCGATACGAGAATTTTGGATCTCATGTGTCCGATTGGAAAAGGACAAAGGGCTCTCATCGTAGCC
CCTCCAAGAACCGGTAAGACGATTCTAATGCAAAATATCGCAAACGCGATCACTTCCAATCATCCGGAATGCACCTTAAT
CGTGCTTTTGATCGACGAACGTCCGGAAGAAGTAACCGACATGGCACGCCACGTACGCGGAGAGGTAGTTTCCTCGACAT
TCGACGAACCGGCACAAAGGCACGTTCAGGTTGCGGAGATGGTCATTGAAAAAGCCAAAAGACTTGTGGAACACGGAAAA
GACGTAGTCATTCTTTTAGATTCGATCACCCGATTGGCAAGAGCCTACAATCAGGTAATCCCGACTTCCGGAAAAATTCT
TTCGGGCGGCGTGGATTCCAACGCTCTTCATAAACCGAAACGATTCTTCGGAGCTGCAAGAAACATAGAAGAAGGCGGCT
CTCTCACAATCATCGCCACCGCATTGATCGATACCGGATCCAAAATGGACGAGGTAATCTTCGAAGAATTCAAAGGAACC
GGAAACATGGAAATCCATCTAGATCGAAAACTTTCCGACAAACGGATTTTCCCAGCCATTGACATCAACAAGTCCGGAAC
CCGTAAGGAAGAGCTCCTTATCACCAAAGATGTTCTTCAGAAAGTGTTCGTTCTGAGAAAAGTACTTTCTCCCATGAGTA
TCACGGAAAGCATGGAACTATTGCTGGAAAAAATGAGGCTTTCTAAAACCAACGATGCCTTTTTGGCCAGCATGAACACC
CAGTAA

Upstream 100 bases:

>100_bases
AGAGCCGATCTAACCGATCTCTTCTCCACTCTGTATTCTCAAAAATAAAATCTCAAAAAGGCGAATCCTTCCCTTTTTAA
AAATTCAAGTAGCTCCTAAT

Downstream 100 bases:

>100_bases
ACGCATCAACAAAGGAATTTATGAAAACCGAAATTCATCCAAACTATAAAGCGGCCAAAATCAGCTGTGCGTCCTGTGGA
ACCGTCTACGAAACCAGAAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MATVRRDNKNKHHNQNNNYSQNETTGSIEEESAGQESQDLESSDNADHRSRKRKRGGYEGPTPAPIDLVELKKKAIADLI
EAAKGLGVENTSGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGD
TVEGQIRPPKESERFFAMLRVETVNGYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVA
PPRTGKTILMQNIANAITSNHPECTLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGK
DVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGT
GNMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNT
Q

Sequences:

>Translated_481_residues
MATVRRDNKNKHHNQNNNYSQNETTGSIEEESAGQESQDLESSDNADHRSRKRKRGGYEGPTPAPIDLVELKKKAIADLI
EAAKGLGVENTSGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGD
TVEGQIRPPKESERFFAMLRVETVNGYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVA
PPRTGKTILMQNIANAITSNHPECTLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGK
DVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGT
GNMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNT
Q
>Mature_480_residues
ATVRRDNKNKHHNQNNNYSQNETTGSIEEESAGQESQDLESSDNADHRSRKRKRGGYEGPTPAPIDLVELKKKAIADLIE
AAKGLGVENTSGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDT
VEGQIRPPKESERFFAMLRVETVNGYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVAP
PRTGKTILMQNIANAITSNHPECTLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGKD
VVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGTG
NMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNTQ

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=64.6634615384615, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1788251, Length=274, Percent_Identity=26.6423357664234, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 53519; Mature: 53388

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVRRDNKNKHHNQNNNYSQNETTGSIEEESAGQESQDLESSDNADHRSRKRKRGGYEG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCHHHHHHHHCCCCCC
PTPAPIDLVELKKKAIADLIEAAKGLGVENTSGLKKQNLIFAILQAQAERDGQVHAAGVM
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
EKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVEGQIRPPKESERFFAMLR
HHCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCHHHHEEEEEE
VETVNGYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVA
EEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEE
PPRTGKTILMQNIANAITSNHPECTLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQR
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHH
HVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPK
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCH
RFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIHLDRKLSDKRIFPAI
HHHHHHCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
DINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNT
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCC
Q
C
>Mature Secondary Structure 
ATVRRDNKNKHHNQNNNYSQNETTGSIEEESAGQESQDLESSDNADHRSRKRKRGGYEG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCHHHHHHHHCCCCCC
PTPAPIDLVELKKKAIADLIEAAKGLGVENTSGLKKQNLIFAILQAQAERDGQVHAAGVM
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
EKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVEGQIRPPKESERFFAMLR
HHCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCHHHHEEEEEE
VETVNGYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVA
EEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEE
PPRTGKTILMQNIANAITSNHPECTLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQR
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHH
HVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPK
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCH
RFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIHLDRKLSDKRIFPAI
HHHHHHCCCCCCCCEEEEEEEEHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
DINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNT
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]