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The map label for this gene is murD

Identifier: 116328899

GI number: 116328899

Start: 2709803

End: 2711191

Strand: Direct

Name: murD

Synonym: LBL_2290

Alternate gene names: 116328899

Gene position: 2709803-2711191 (Clockwise)

Preceding gene: 116328895

Following gene: 116328908

Centisome position: 74.97

GC content: 41.83

Gene sequence:

>1389_bases
ATGAAATTTCCCGAGTCCTTGAAAGGCTTAAAGATTCTTGTGTTGGGAGGAGGTATCTCCGGAAACTCCGCACTTGATTT
TTTAATCTCCGAAGAAGCACAACCAATTCTATGTGATCGAAATCAACCCGAAACGATCTCCGTCCCTTTTTTTCACGACA
ACATCGATCCACGATCTTTTTTTGAAATTTCATTGATCATCAAATCTCCCGGAATTTTACCGACACACCCGATTCTTTCT
TATGCAGTCGAAAAGAAGATTCCCGTGTTTTCCGAAATTGATCTCGGCAGATTTTTTTTTAAAGGGAAAATCATCGGTAT
TACAGGAACGGACGGAAAATCGACGACAACTTCTCTGGTTGCTCATCTTCTGAAAAAAGATTTTTCCGATCTTAAAGAAG
GCGGAAATCTGGGAATCCCATTTACTTCCTTCTGCAAAGAACCGATTTCCTTAGCCGTCTTAGAACTTTCCAGTTATCAA
CTCGAAGATTCGTCTCCGCTTGATTTGAATGTGTCCGTGTTCCTAAATCTCGCTCCCGATCATCTAGAAAGACACGAAAC
GATGGAAAATTATTTTCGTGCAAAACTTAAAATTGCCGATCTTCAAAACCCGAATCATTCATTGATCGTCTCCGAAAAAA
TCAGGGAGAAAATTCTAAATTCCACTTCCGTTCAGTGTAAGTTATTGAGTTTTGGAAGAGCTACGACATCGGAGGCGATT
TTAGACGAGAGTTCATCGGAGATACGGACTTCGAAATTCATCTACGACATTTCTCGATTTTATCTTCCGGGAACTCATAA
CCGTGAAAATCTAGCGGCGGCGATTCTTGCATCGGAAGCGATCGGAGGAAAACCGGAATCGATCCAAGCGCAGATTCCAT
TTTTTATGGGACTTCCCCATCGTTTTCAAATCGCAGGAGAAAAACGAGGTATTTCGTTTATCAACGATTCCAAATCCACA
AACCTACACAGCATGCTCGCGGGAATGTCCGCTTGGAAGAATCTAGATCGAACCTGTCTGATCCTCGGAGGTAGACCGAA
ACAGGAAGACCCGAAACCGCTTTATGATTTTCTAATGCGTGGAATAGGTTGTGTAGTTTTAATCGGAGAAGCGCGTTCGG
TCTGGGAAAAAGGGATCCGAAACGTGATCGGAGAAAAACTATTCTCGGTCGAAAACTTAGATGAGGCATTTAAAATATTC
AAAAAATGGAATGTTATTTCGGAATCGCCCGAGATCCGCAAAATTCGTCTTTCAAGCGAAATCACAATTTCGTATTTCGT
TTTCTCGCCGGCCTGCGTCAGTTTCGATCAATACAAAAACTTCGAAGAAAGAGGCAACCACTTCCTTTCTTTGGTAGAGG
ATTTCTTAAATAACGCGAGTTGGACATAA

Upstream 100 bases:

>100_bases
CTTGTACTTTATGCTCAAGCAAGGGAACATCCTTAGTTTTTGCTACTGACAACTATCGAGGACGAAAATACCCTGTCAAT
CACTGTTCAACGGTTTAAAA

Downstream 100 bases:

>100_bases
CAAAATGCGAAAGCGATTGTAGTGGAAATTCCGAAGGAATTGGAGCATTATGTTGCGACCTGAAACGGCGCCCCTTAGGA
AGCCGTTTCACTGAGTTTAG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKFPESLKGLKILVLGGGISGNSALDFLISEEAQPILCDRNQPETISVPFFHDNIDPRSFFEISLIIKSPGILPTHPILS
YAVEKKIPVFSEIDLGRFFFKGKIIGITGTDGKSTTTSLVAHLLKKDFSDLKEGGNLGIPFTSFCKEPISLAVLELSSYQ
LEDSSPLDLNVSVFLNLAPDHLERHETMENYFRAKLKIADLQNPNHSLIVSEKIREKILNSTSVQCKLLSFGRATTSEAI
LDESSSEIRTSKFIYDISRFYLPGTHNRENLAAAILASEAIGGKPESIQAQIPFFMGLPHRFQIAGEKRGISFINDSKST
NLHSMLAGMSAWKNLDRTCLILGGRPKQEDPKPLYDFLMRGIGCVVLIGEARSVWEKGIRNVIGEKLFSVENLDEAFKIF
KKWNVISESPEIRKIRLSSEITISYFVFSPACVSFDQYKNFEERGNHFLSLVEDFLNNASWT

Sequences:

>Translated_462_residues
MKFPESLKGLKILVLGGGISGNSALDFLISEEAQPILCDRNQPETISVPFFHDNIDPRSFFEISLIIKSPGILPTHPILS
YAVEKKIPVFSEIDLGRFFFKGKIIGITGTDGKSTTTSLVAHLLKKDFSDLKEGGNLGIPFTSFCKEPISLAVLELSSYQ
LEDSSPLDLNVSVFLNLAPDHLERHETMENYFRAKLKIADLQNPNHSLIVSEKIREKILNSTSVQCKLLSFGRATTSEAI
LDESSSEIRTSKFIYDISRFYLPGTHNRENLAAAILASEAIGGKPESIQAQIPFFMGLPHRFQIAGEKRGISFINDSKST
NLHSMLAGMSAWKNLDRTCLILGGRPKQEDPKPLYDFLMRGIGCVVLIGEARSVWEKGIRNVIGEKLFSVENLDEAFKIF
KKWNVISESPEIRKIRLSSEITISYFVFSPACVSFDQYKNFEERGNHFLSLVEDFLNNASWT
>Mature_462_residues
MKFPESLKGLKILVLGGGISGNSALDFLISEEAQPILCDRNQPETISVPFFHDNIDPRSFFEISLIIKSPGILPTHPILS
YAVEKKIPVFSEIDLGRFFFKGKIIGITGTDGKSTTTSLVAHLLKKDFSDLKEGGNLGIPFTSFCKEPISLAVLELSSYQ
LEDSSPLDLNVSVFLNLAPDHLERHETMENYFRAKLKIADLQNPNHSLIVSEKIREKILNSTSVQCKLLSFGRATTSEAI
LDESSSEIRTSKFIYDISRFYLPGTHNRENLAAAILASEAIGGKPESIQAQIPFFMGLPHRFQIAGEKRGISFINDSKST
NLHSMLAGMSAWKNLDRTCLILGGRPKQEDPKPLYDFLMRGIGCVVLIGEARSVWEKGIRNVIGEKLFSVENLDEAFKIF
KKWNVISESPEIRKIRLSSEITISYFVFSPACVSFDQYKNFEERGNHFLSLVEDFLNNASWT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=459, Percent_Identity=28.7581699346405, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_LEPBJ (Q04UG6)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800212.1
- ProteinModelPortal:   Q04UG6
- SMR:   Q04UG6
- STRING:   Q04UG6
- GeneID:   4409685
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0789
- eggNOG:   COG0771
- HOGENOM:   HBG529092
- OMA:   FQVGRHR
- PhylomeDB:   Q04UG6
- ProtClustDB:   CLSK573665
- BioCyc:   LBOR355277:LBJ_0789-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51696; Mature: 51696

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPESLKGLKILVLGGGISGNSALDFLISEEAQPILCDRNQPETISVPFFHDNIDPRSF
CCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCE
FEISLIIKSPGILPTHPILSYAVEKKIPVFSEIDLGRFFFKGKIIGITGTDGKSTTTSLV
EEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
AHLLKKDFSDLKEGGNLGIPFTSFCKEPISLAVLELSSYQLEDSSPLDLNVSVFLNLAPD
HHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEEEEEECCEECCCCCCCEEEEEEEEEECHH
HLERHETMENYFRAKLKIADLQNPNHSLIVSEKIREKILNSTSVQCKLLSFGRATTSEAI
HHHHHHHHHHHHHHEEEEEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
LDESSSEIRTSKFIYDISRFYLPGTHNRENLAAAILASEAIGGKPESIQAQIPFFMGLPH
HCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCC
RFQIAGEKRGISFINDSKSTNLHSMLAGMSAWKNLDRTCLILGGRPKQEDPKPLYDFLMR
EEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
GIGCVVLIGEARSVWEKGIRNVIGEKLFSVENLDEAFKIFKKWNVISESPEIRKIRLSSE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEECCC
ITISYFVFSPACVSFDQYKNFEERGNHFLSLVEDFLNNASWT
EEEEEEEECCHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFPESLKGLKILVLGGGISGNSALDFLISEEAQPILCDRNQPETISVPFFHDNIDPRSF
CCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCCCE
FEISLIIKSPGILPTHPILSYAVEKKIPVFSEIDLGRFFFKGKIIGITGTDGKSTTTSLV
EEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
AHLLKKDFSDLKEGGNLGIPFTSFCKEPISLAVLELSSYQLEDSSPLDLNVSVFLNLAPD
HHHHHHHHHHHHCCCCCCCCHHHHHCCCHHEEEEEECCEECCCCCCCEEEEEEEEEECHH
HLERHETMENYFRAKLKIADLQNPNHSLIVSEKIREKILNSTSVQCKLLSFGRATTSEAI
HHHHHHHHHHHHHHEEEEEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
LDESSSEIRTSKFIYDISRFYLPGTHNRENLAAAILASEAIGGKPESIQAQIPFFMGLPH
HCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCC
RFQIAGEKRGISFINDSKSTNLHSMLAGMSAWKNLDRTCLILGGRPKQEDPKPLYDFLMR
EEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
GIGCVVLIGEARSVWEKGIRNVIGEKLFSVENLDEAFKIFKKWNVISESPEIRKIRLSSE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEECCC
ITISYFVFSPACVSFDQYKNFEERGNHFLSLVEDFLNNASWT
EEEEEEEECCHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA