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The map label for this gene is carA

Identifier: 116328742

GI number: 116328742

Start: 2475767

End: 2476858

Strand: Direct

Name: carA

Synonym: LBL_2116

Alternate gene names: 116328742

Gene position: 2475767-2476858 (Clockwise)

Preceding gene: 116328740

Following gene: 116328743

Centisome position: 68.5

GC content: 44.23

Gene sequence:

>1092_bases
ATGAAAGCGTTCTTGGTTCTCGACAACGGCATGATCTTAGAAGGAGAATCCTTCGGCTATGAAAGCGAATCCGTTGGTGA
AATTGTTTTTAATACTTCCATGGCGGGTTATCAAGAGATCCTAACGGATCCTTCCTATTGCAACCAGATTATAACTCTAA
CTTATCCTATGATCGGGAACTACGGAATTCATCCGGACAACATGGAATCTTCCAAAATCCAAGCGAGCGGACTGATCGTA
AGAGAATATGTGGATCTCCCTTCCAACTTCATGTCCCAAAAAACCCTTTCCAAATTTTTAAAAGAATATCAAATCCCCGC
CATCCAAGGTATCGACACAAGAAAACTGACGCGTTACATTCGAACAAACGGTTCCCCGAACGGTGGAATTTTCGTGGCTC
GAGAATACTCCCCTGAATTTTTGGAGAAGGTAAAATCCTTTCCGGGAATCACGGGCACCGATCTTGCAAAGGTCGTAACC
ACTTCCACGAAATATATCTTTGGAACTCACACCGGAAAAAAATACAAACTTGCAGTCTATGATTATGGAGTGAAAACGAA
CATTCTCCGTTTATTAGATGCAGCAGGTTTTGCCGTGACCGTTTATCCGGCCTTGACTCCCGCGGAAGAAATTATGAAAG
AAGGAACGGACGCATTCTTTCTTTCAAACGGCCCCGGAGATCCGGCTCCACTTGACTACGCGATCGACGCGACCCGCAAG
ATTATGGAAAAAGGTTATCCTCTATTCGGGATCTGTCTCGGACATCAAATCATCGGGCTTTCTTTAGGAAAAAAAACCGA
GAAGATGAAATTCGGACATCGCGGAGGAAATCAACCGGTTAAGAATTTATCGACCGGACAAGTGGAGATCACTTCGCAAA
ATCACGGTTTTGCGGTAATAGACGACGGGAAATCGAGCGAGCCCGTTTCGTTTTTAAATCTAAACGACAACACCGTCGAA
GGAATTTTGAAATCGGGTTATCCTTTGCTTACGGTTCAATATCATCCGGAAAGCGCGCCGGGCCCGAACGATTCCCGTTA
CCTATTCCAGAAATTTTATGATCTTGTAGAAACTACAAAAAGAGGCAAATAG

Upstream 100 bases:

>100_bases
CGATTCGAATCACACCCATTCCTCTTTGCCGGAATTTTGTCCGCTTTTTATCCTCCGTTTAGTGTTGACTATAGTCACCA
TCTCATCATCTTTGCAGAAG

Downstream 100 bases:

>100_bases
TCGATGGCAAATTTTCCACAACTTCCCGATAGAATTCTCGGCCTTCCAATTGGAAAAGATTTTTCCATAGAGGCCCTCGT
CAAAGACCTCTGGAATCCGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLIV
REYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYIRTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVT
TSTKYIFGTHTGKKYKLAVYDYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRK
IMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVIDDGKSSEPVSFLNLNDNTVE
GILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTKRGK

Sequences:

>Translated_363_residues
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLIV
REYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYIRTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVT
TSTKYIFGTHTGKKYKLAVYDYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRK
IMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVIDDGKSSEPVSFLNLNDNTVE
GILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTKRGK
>Mature_363_residues
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLIV
REYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYIRTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVT
TSTKYIFGTHTGKKYKLAVYDYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRK
IMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVIDDGKSSEPVSFLNLNDNTVE
GILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTKRGK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=372, Percent_Identity=40.3225806451613, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI21361331, Length=374, Percent_Identity=38.5026737967914, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI169790915, Length=374, Percent_Identity=38.5026737967914, Blast_Score=241, Evalue=7e-64,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=47.3404255319149, Blast_Score=342, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI193204318, Length=376, Percent_Identity=39.3617021276596, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324878, Length=373, Percent_Identity=38.3378016085791, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=37.3401534526854, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI45555749, Length=378, Percent_Identity=41.5343915343915, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=41.5343915343915, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_LEPBJ (Q04R57)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801371.1
- ProteinModelPortal:   Q04R57
- SMR:   Q04R57
- STRING:   Q04R57
- GeneID:   4410372
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2119
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   EADIPFF
- PhylomeDB:   Q04R57
- ProtClustDB:   PRK12564
- BioCyc:   LBOR355277:LBJ_2119-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40053; Mature: 40053

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 252-252 ACT_SITE 335-335 ACT_SITE 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGN
CEEEEEECCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHCEEEEEEECCCCC
YGIHPDNMESSKIQASGLIVREYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYI
CCCCCCCCCCCEEECCCEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVTTSTKYIFGTHTGKKYKLAVY
HCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEE
DYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRK
ECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
IMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVI
HHHCCCCEEEEECCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
DDGKSSEPVSFLNLNDNTVEGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTK
ECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
RGK
CCC
>Mature Secondary Structure
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGN
CEEEEEECCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHCEEEEEEECCCCC
YGIHPDNMESSKIQASGLIVREYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYI
CCCCCCCCCCCEEECCCEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVTTSTKYIFGTHTGKKYKLAVY
HCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEEE
DYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRK
ECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
IMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVI
HHHCCCCEEEEECCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
DDGKSSEPVSFLNLNDNTVEGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTK
ECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC
RGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA