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The map label for this gene is murF [H]

Identifier: 116328698

GI number: 116328698

Start: 2426655

End: 2428055

Strand: Direct

Name: murF [H]

Synonym: LBL_2070

Alternate gene names: 116328698

Gene position: 2426655-2428055 (Clockwise)

Preceding gene: 116328694

Following gene: 116328699

Centisome position: 67.14

GC content: 42.04

Gene sequence:

>1401_bases
ATGAAGGCGACCTTTTCCTACGACCCGGAAACCGTCCGCAAAGTATTGGGATTCGCATCGGATGCCCGGTTTTATAAGGA
ATCACCGATTACGACTGTTACGACCGCTTCCACCGAGGTGGAAAAAAATTCTCTTTTCATTCCTCTCCGAGGGAATCGGG
ACGGACACGAATTTATCGGAGACGCACTTTCTCGCGGAGCGAGTTATTTTTTAGTCGAAGAAGACCATCCGATTCGAAAG
AACTTCGATTCGAAGGAGCGGGCTAAAGCGATTCCCGTAAAGGACACGTTAGTTTCACTTGGGAAGCTCGCCGCCTTCCA
CAGATCACGTTTTAATCCGATCGTAATTGCAGTTACCGGATCCAGCGGAAAGACCACGACGAAGGAAATTTTAGGAAACT
GTTTAAAAAATCTCGAAGAACCCGTATTGGTCGTCACTGAAAAGAATTACAATAACGAAATCGGGCTTCCGTTCACTTTG
TTTCGAATTTCCGACCGAACTCGAATCGTAGTATGCGAACTCGGAATGAATCACAAAGGGGAAATTGCAAGACTCACTCA
AATTGCAAAACCGGATTTCGCTCTAATTACTACAATTGGAACCGCACACATAGAATTTTTAGGAAGCCAAAAAAACATCG
CAAAAGCAAAAGTGGAAGTGGCTGAGGGTTTGGTGAAGGGAGGAAAACTCTTTTATCCAGATACGGGAGAATATTCTAAA
ATACTAAAAAGTACACTCCGCCGACATGGAAGCAGATTGATTCTCGTTAAACCGAATCATATATTTTCCGTTCTTCAAAA
AAGACCTTCCGGATTCTTATTGGAATACAGGGACAAAGAGATTGAATGGAATCTACCCGGATATAAACTTCTAGAAAATT
TAGCGATCGCAATCGCTTGTTTAGAAACTGTCGGAGTTCCTACGGAATGGATTCTAAAAGGAATCTCCACGTTTAAATCC
GTCGATAAAAGACTGAACTTACAAAAAGGGAAATACTCCATAATTAACGACACTTACAATGCGAACTACGAATCGATGCT
TTCATCCTTGGAAGTTACCGATCAGCTCGCGGATGGAAAAGAATTCTACGTAGTTTTAGGAGATATGAAAGAATTAGGAA
AACACTCCCAAAAGTTTCATAAGGAACTCGGAAAAAAGTGTTCCGAATTTCAGAATTTAAAAGGACTTTTTACATTTGGA
ACCGACTCTCTTTGGATTCGGGAAGAATTTGTTAAACGAACAACCTTTCCCAGATTTTCCGAACATTTTGCCGGAACCGA
AGAAGGGCTTGTAAAACTCGTTCGGAAATTTTCGCAAACGGTTCCGAAAGGTTCCATCGTTTTAGCGAAAGCCTCGCGCG
GAATTCAATTGGAGCGCTTCGTAGATTCTCTTTCTGTTTAA

Upstream 100 bases:

>100_bases
TGAATCCATTTTCGATCTTTACAAAAGTTTATTCTTAATTATTCAGACAAATCGTTTATTTTAGAATTTTCAAAACATCC
CGCGTCTACAATCTGGATTC

Downstream 100 bases:

>100_bases
TACTTGCAATCACTACGATCGAAGGAAATATCTTGAACAGGAGAAAAGACTTGAGCGCAAAAGTGGCAATCATAATGGGA
AGTCATTCCGATTGGGAAAC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKATFSYDPETVRKVLGFASDARFYKESPITTVTTASTEVEKNSLFIPLRGNRDGHEFIGDALSRGASYFLVEEDHPIRK
NFDSKERAKAIPVKDTLVSLGKLAAFHRSRFNPIVIAVTGSSGKTTTKEILGNCLKNLEEPVLVVTEKNYNNEIGLPFTL
FRISDRTRIVVCELGMNHKGEIARLTQIAKPDFALITTIGTAHIEFLGSQKNIAKAKVEVAEGLVKGGKLFYPDTGEYSK
ILKSTLRRHGSRLILVKPNHIFSVLQKRPSGFLLEYRDKEIEWNLPGYKLLENLAIAIACLETVGVPTEWILKGISTFKS
VDKRLNLQKGKYSIINDTYNANYESMLSSLEVTDQLADGKEFYVVLGDMKELGKHSQKFHKELGKKCSEFQNLKGLFTFG
TDSLWIREEFVKRTTFPRFSEHFAGTEEGLVKLVRKFSQTVPKGSIVLAKASRGIQLERFVDSLSV

Sequences:

>Translated_466_residues
MKATFSYDPETVRKVLGFASDARFYKESPITTVTTASTEVEKNSLFIPLRGNRDGHEFIGDALSRGASYFLVEEDHPIRK
NFDSKERAKAIPVKDTLVSLGKLAAFHRSRFNPIVIAVTGSSGKTTTKEILGNCLKNLEEPVLVVTEKNYNNEIGLPFTL
FRISDRTRIVVCELGMNHKGEIARLTQIAKPDFALITTIGTAHIEFLGSQKNIAKAKVEVAEGLVKGGKLFYPDTGEYSK
ILKSTLRRHGSRLILVKPNHIFSVLQKRPSGFLLEYRDKEIEWNLPGYKLLENLAIAIACLETVGVPTEWILKGISTFKS
VDKRLNLQKGKYSIINDTYNANYESMLSSLEVTDQLADGKEFYVVLGDMKELGKHSQKFHKELGKKCSEFQNLKGLFTFG
TDSLWIREEFVKRTTFPRFSEHFAGTEEGLVKLVRKFSQTVPKGSIVLAKASRGIQLERFVDSLSV
>Mature_466_residues
MKATFSYDPETVRKVLGFASDARFYKESPITTVTTASTEVEKNSLFIPLRGNRDGHEFIGDALSRGASYFLVEEDHPIRK
NFDSKERAKAIPVKDTLVSLGKLAAFHRSRFNPIVIAVTGSSGKTTTKEILGNCLKNLEEPVLVVTEKNYNNEIGLPFTL
FRISDRTRIVVCELGMNHKGEIARLTQIAKPDFALITTIGTAHIEFLGSQKNIAKAKVEVAEGLVKGGKLFYPDTGEYSK
ILKSTLRRHGSRLILVKPNHIFSVLQKRPSGFLLEYRDKEIEWNLPGYKLLENLAIAIACLETVGVPTEWILKGISTFKS
VDKRLNLQKGKYSIINDTYNANYESMLSSLEVTDQLADGKEFYVVLGDMKELGKHSQKFHKELGKKCSEFQNLKGLFTFG
TDSLWIREEFVKRTTFPRFSEHFAGTEEGLVKLVRKFSQTVPKGSIVLAKASRGIQLERFVDSLSV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=453, Percent_Identity=30.0220750551876, Blast_Score=145, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 52329; Mature: 52329

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATFSYDPETVRKVLGFASDARFYKESPITTVTTASTEVEKNSLFIPLRGNRDGHEFIG
CCCCCCCCHHHHHHHHCCHHCCHHHHCCCCEEEEECCCEECCCEEEEEECCCCCHHHHHH
DALSRGASYFLVEEDHPIRKNFDSKERAKAIPVKDTLVSLGKLAAFHRSRFNPIVIAVTG
HHHHCCCEEEEEECCCCHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SSGKTTTKEILGNCLKNLEEPVLVVTEKNYNNEIGLPFTLFRISDRTRIVVCELGMNHKG
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
EIARLTQIAKPDFALITTIGTAHIEFLGSQKNIAKAKVEVAEGLVKGGKLFYPDTGEYSK
CHHHHHHHCCCCEEEEEEECHHHHHEECCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHH
ILKSTLRRHGSRLILVKPNHIFSVLQKRPSGFLLEYRDKEIEWNLPGYKLLENLAIAIAC
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHH
LETVGVPTEWILKGISTFKSVDKRLNLQKGKYSIINDTYNANYESMLSSLEVTDQLADGK
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCC
EFYVVLGDMKELGKHSQKFHKELGKKCSEFQNLKGLFTFGTDSLWIREEFVKRTTFPRFS
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCHH
EHFAGTEEGLVKLVRKFSQTVPKGSIVLAKASRGIQLERFVDSLSV
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKATFSYDPETVRKVLGFASDARFYKESPITTVTTASTEVEKNSLFIPLRGNRDGHEFIG
CCCCCCCCHHHHHHHHCCHHCCHHHHCCCCEEEEECCCEECCCEEEEEECCCCCHHHHHH
DALSRGASYFLVEEDHPIRKNFDSKERAKAIPVKDTLVSLGKLAAFHRSRFNPIVIAVTG
HHHHCCCEEEEEECCCCHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
SSGKTTTKEILGNCLKNLEEPVLVVTEKNYNNEIGLPFTLFRISDRTRIVVCELGMNHKG
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
EIARLTQIAKPDFALITTIGTAHIEFLGSQKNIAKAKVEVAEGLVKGGKLFYPDTGEYSK
CHHHHHHHCCCCEEEEEEECHHHHHEECCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHH
ILKSTLRRHGSRLILVKPNHIFSVLQKRPSGFLLEYRDKEIEWNLPGYKLLENLAIAIAC
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHH
LETVGVPTEWILKGISTFKSVDKRLNLQKGKYSIINDTYNANYESMLSSLEVTDQLADGK
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCC
EFYVVLGDMKELGKHSQKFHKELGKKCSEFQNLKGLFTFGTDSLWIREEFVKRTTFPRFS
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCHH
EHFAGTEEGLVKLVRKFSQTVPKGSIVLAKASRGIQLERFVDSLSV
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]