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The map label for this gene is rlmL [H]

Identifier: 116328650

GI number: 116328650

Start: 2354174

End: 2355418

Strand: Direct

Name: rlmL [H]

Synonym: LBL_2016

Alternate gene names: 116328650

Gene position: 2354174-2355418 (Clockwise)

Preceding gene: 116328649

Following gene: 116328651

Centisome position: 65.13

GC content: 41.53

Gene sequence:

>1245_bases
GTGACAAATTCTAAAAAACCAATTCCTAAAAAATCTCTTCGATTAAAATCAAAAAGATCCGTTATAAAAAATTTTCCGAA
CGAACCAAATCAAAAGCAAAATCCTTTTAAATCGGAATGGGATTTTTCAAAACCGGATTCTTTCGAATATCATGCATCCT
GCCCGGACGGACTTTCCGGGCTTTTAAAAGAAGAAATTTTAGAAGCCGGTCTAAAGATCATTTCCGAAAATAGAGGCGGA
GTTTTTTTTCAAGGTTCGGCGAGCGCCCTCAAGGAATTCGCTCTTTCTACTGGAATCTCATCAGGGATCAGTATTTCTTT
AAAATACTGGAAAGTGGAAAGTCCGGAAGATCTTTATAACCAGGCTGTCCAATTTCCGTTCGAGAAAATCTTCGGACCCG
AATATTCTTTTCGAATCGATTCGACGACCAAGGATTCTTTGAAGGACTCGCGTTATGCGACATACAAACTCAAAGACGCC
ATCTTTGATCGATTTCGTTCCAAAGGAAAAGAACCCCCGAAGGTCTCTCGAGACGAACCTGATTTGTTATTCTATCTTCG
CTCCCATTCCGACCATGCAAAACTTTCCCTGGGTCTGAACACAAGGCCTCTTCAACAAAGAGGACATGGAAGAATCGGAG
GTCCGGCTCCTATGCGGGAGATTCTCGCATCCGCTTTGGTTCGATACTCGGGATGGAACACCAGATCAGTTTTATATGAT
CCGTTCTGTGGTTCGGGGACCATCGTAGTAGAAGCGGCTCTCAAACTTCTATACGGGGGTTACACGAATTACAGAAGTTT
GTCGTCTTCCCTTTCGTTTAAGAAACTCTTCGGGGAACCGATCCTCAATGTAAAGAATTATTCCGAAATGAAAATTTTCG
CGTCCGACAAGAATGAAACGATTTTGAATCTTGCAAGACAAAATGCAAAGAACGCAGGGGTGGATCACCTGATCACTTTT
TTCGAATCGGACGCTACTATTTCCGAAAATGAGAAAAAAATTTCCGACGGTTTTATTGTGACCAATCCTCCTTACGGAGT
TCGAATAGGTACCAAAGAAGAAGCCAAGGAAATTTATGTTGCTTGGGGGAAAAAACTCAAAGATCATTTTACGGGAAATA
TCTTGGCACTCGCTTGCGGAGATACTTCGCTCTTAGGTTTTCTCAAATTGAAAAAGGATAAGGAGCAATCCCTGACAATT
GGTAAGCTCAAAGGAAAATTAGTTGCCTACACTTTGGGTAAATAA

Upstream 100 bases:

>100_bases
AAATTCTCGTCTCGGAAGAAGAACACATCGATTGGATCGAATCCCAACAAGAAATCATCCGTCAGATTGGCGTTGAAAAC
TATCTCGCGCAGCAAATAGA

Downstream 100 bases:

>100_bases
TCCAATCGAACCGGAATGAAACATCAAGAGATTCTCCTTAAACTTTTGGAAGTTACGGATAATACTAAGGATTCGTTTCA
ATTTTTAAAACTTTTTCGTT

Product: N6-adenine-specific DNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTNSKKPIPKKSLRLKSKRSVIKNFPNEPNQKQNPFKSEWDFSKPDSFEYHASCPDGLSGLLKEEILEAGLKIISENRGG
VFFQGSASALKEFALSTGISSGISISLKYWKVESPEDLYNQAVQFPFEKIFGPEYSFRIDSTTKDSLKDSRYATYKLKDA
IFDRFRSKGKEPPKVSRDEPDLLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGPAPMREILASALVRYSGWNTRSVLYD
PFCGSGTIVVEAALKLLYGGYTNYRSLSSSLSFKKLFGEPILNVKNYSEMKIFASDKNETILNLARQNAKNAGVDHLITF
FESDATISENEKKISDGFIVTNPPYGVRIGTKEEAKEIYVAWGKKLKDHFTGNILALACGDTSLLGFLKLKKDKEQSLTI
GKLKGKLVAYTLGK

Sequences:

>Translated_414_residues
MTNSKKPIPKKSLRLKSKRSVIKNFPNEPNQKQNPFKSEWDFSKPDSFEYHASCPDGLSGLLKEEILEAGLKIISENRGG
VFFQGSASALKEFALSTGISSGISISLKYWKVESPEDLYNQAVQFPFEKIFGPEYSFRIDSTTKDSLKDSRYATYKLKDA
IFDRFRSKGKEPPKVSRDEPDLLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGPAPMREILASALVRYSGWNTRSVLYD
PFCGSGTIVVEAALKLLYGGYTNYRSLSSSLSFKKLFGEPILNVKNYSEMKIFASDKNETILNLARQNAKNAGVDHLITF
FESDATISENEKKISDGFIVTNPPYGVRIGTKEEAKEIYVAWGKKLKDHFTGNILALACGDTSLLGFLKLKKDKEQSLTI
GKLKGKLVAYTLGK
>Mature_413_residues
TNSKKPIPKKSLRLKSKRSVIKNFPNEPNQKQNPFKSEWDFSKPDSFEYHASCPDGLSGLLKEEILEAGLKIISENRGGV
FFQGSASALKEFALSTGISSGISISLKYWKVESPEDLYNQAVQFPFEKIFGPEYSFRIDSTTKDSLKDSRYATYKLKDAI
FDRFRSKGKEPPKVSRDEPDLLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGPAPMREILASALVRYSGWNTRSVLYDP
FCGSGTIVVEAALKLLYGGYTNYRSLSSSLSFKKLFGEPILNVKNYSEMKIFASDKNETILNLARQNAKNAGVDHLITFF
ESDATISENEKKISDGFIVTNPPYGVRIGTKEEAKEIYVAWGKKLKDHFTGNILALACGDTSLLGFLKLKKDKEQSLTIG
KLKGKLVAYTLGK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=333, Percent_Identity=31.2312312312312, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46305; Mature: 46174

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKKPIPKKSLRLKSKRSVIKNFPNEPNQKQNPFKSEWDFSKPDSFEYHASCPDGLSG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
LLKEEILEAGLKIISENRGGVFFQGSASALKEFALSTGISSGISISLKYWKVESPEDLYN
HHHHHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
QAVQFPFEKIFGPEYSFRIDSTTKDSLKDSRYATYKLKDAIFDRFRSKGKEPPKVSRDEP
HHHHCCHHHHCCCCEEEEECCCCHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
DLLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGPAPMREILASALVRYSGWNTRSVLYD
CEEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEC
PFCGSGTIVVEAALKLLYGGYTNYRSLSSSLSFKKLFGEPILNVKNYSEMKIFASDKNET
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
ILNLARQNAKNAGVDHLITFFESDATISENEKKISDGFIVTNPPYGVRIGTKEEAKEIYV
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHH
AWGKKLKDHFTGNILALACGDTSLLGFLKLKKDKEQSLTIGKLKGKLVAYTLGK
HHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHCEEEEECCCEEEEEECCC
>Mature Secondary Structure 
TNSKKPIPKKSLRLKSKRSVIKNFPNEPNQKQNPFKSEWDFSKPDSFEYHASCPDGLSG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHH
LLKEEILEAGLKIISENRGGVFFQGSASALKEFALSTGISSGISISLKYWKVESPEDLYN
HHHHHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
QAVQFPFEKIFGPEYSFRIDSTTKDSLKDSRYATYKLKDAIFDRFRSKGKEPPKVSRDEP
HHHHCCHHHHCCCCEEEEECCCCHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
DLLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGPAPMREILASALVRYSGWNTRSVLYD
CEEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEC
PFCGSGTIVVEAALKLLYGGYTNYRSLSSSLSFKKLFGEPILNVKNYSEMKIFASDKNET
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHH
ILNLARQNAKNAGVDHLITFFESDATISENEKKISDGFIVTNPPYGVRIGTKEEAKEIYV
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHH
AWGKKLKDHFTGNILALACGDTSLLGFLKLKKDKEQSLTIGKLKGKLVAYTLGK
HHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHCEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA