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The map label for this gene is gppA [H]

Identifier: 116328604

GI number: 116328604

Start: 2290045

End: 2291613

Strand: Direct

Name: gppA [H]

Synonym: LBL_1961

Alternate gene names: 116328604

Gene position: 2290045-2291613 (Clockwise)

Preceding gene: 116328597

Following gene: 116328606

Centisome position: 63.36

GC content: 44.17

Gene sequence:

>1569_bases
ATGGTTCAGGAAAAACCTCTCGCGGCCATAGATCTCGGCACCAATTCATTCCACATGATCATCGTTCGAGTTAGAACGAA
CGGAACCTTCGAAGCCATCGCAAGAGAAAAAGAATCGGTTCGCTTAGGTAACAGGTTACAAGAAAACGGTCCGATCGACT
CGGATTCTTTTCGAAGAGGAATCGATTGCCTCAAACGATTTAAAATTCTCGCGGAAAACGCCGGTGCGGAAATCAGAGCC
GTCGCCACAAGCGCGTTACGAGAAGCTTCCAATCGGGAAGAATTCTTAGAAGTTGTATACCAAGAAACGGGCATATCCAT
CGACGTAGTAAGCGGTTATGAAGAAGCCCGTTTGATCTATTTCGGAATCCTTCAAGGAATTCCCGTTTTCGATCGAAAGA
TCATGATGATCGACATCGGTGGAGGAAGTACGGAAATACTCGTCGGCCACAGAGGCAACATTCTATTCTCTAAAAGTTTT
AAACTGGGAGCGATTCGACTCACAGAAAAATTCTTTCAGGAAGAACCTCTTTCCAACTCCGGTATCCGAAAGTGCAGACT
CTTTATAGAAGAAATGCTTCTTCCCTTTCGAAAGATTCTCAGAGATTTAAAACCGGATTTGGTCGTGGGTTCGTCCGGCA
CCATCCAAGCGATCGCCGGAATGATTCTTGCGTCAAGAGGTGAAAGCGAAGAAATTTCATTGAATAACTTTTCCTTTCTG
ACCTCGGAATTTAAGAAAATCCGAAACCTAATTTTGGAAGCGGACACATCTAGAAAAAGGACCAAGATTCCCGGACTCGA
TAGCAAACGAGCCGACATCATTATCGGAGGAACTCTTATCCTGGAGGAAATTTTCGATCTGGCAAACGTTCCCGCTTTGA
CGGTTTCCGAGTTCGCCCTCCGAGAAGGAATCGTTTACGATACCATTCGCAAATGGGAACATTTTGAAAGTAGGGAACAC
TCCAAACATCTGGACGCAATTCGACAAACCTCGGTGGATAACTTCATGCGAGCCTTTTCTAGAGACGAAGAATATTCCAA
ACATGTCGCCAATTTGAGTCTTCAGATCTTCGATCAACTGGCAAGTCTGCATAAACTCGGTCAAGAACAGAGAGAACTTT
TGGAAGCCGCTTCTCTTCTTCACGAAATCGGTCAATCCATCTCTCATTCCGCATATCACAAACACAGTTATTATATGATT
CGAAATTCGGAAATGCTCTTAGGTTTTACCTTTTTGGAAATCGAAATCATCGCTCTCACCGCTCGTTATCATAGAAAAAA
TATTCCGAAACTGAAACATCGGGAGTTCAACAAACTCGCGGAGAAAAATCGGAACCTGGTCTGTCAGCTTTCCGCGATCT
TAAGAATCAGCTCGGCGCTGAATCGTAACCGAGGCGGACTGGTTCCTTCGGTAACTTGTAAGACGGAAAAAAATCAGATC
CGTTTTGTCTTACACACAAACAAGGGTTACGATCCTTCTTTGGAAATCTGGGCGGCGGAAGAACAAAAAATCGCGTTTGA
AGAAACCTTCGGTTATTCGGTGGAATTTGTAACGGGAAATGTAAGATAA

Upstream 100 bases:

>100_bases
CAATAATTTCAACGCTAAGGTTCCCGGCAACCTCAAAACAAAAAAATCAGAAAACAAATTAGGCTGGATTCATTTCAGCG
AAGAAACATTTTGTTCCATC

Downstream 100 bases:

>100_bases
AAGTTGTTCTTCTTTTCTAATATTCGTTTTATTTAACGTGAATTCGGCGTAAGAATCCATGATTCATTTTTCTAAAAACA
AGTTGGGTTTGAACTTTGTA

Product: guanosine-5'-triphosphate, 3'-diphosphatepyrophosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MVQEKPLAAIDLGTNSFHMIIVRVRTNGTFEAIAREKESVRLGNRLQENGPIDSDSFRRGIDCLKRFKILAENAGAEIRA
VATSALREASNREEFLEVVYQETGISIDVVSGYEEARLIYFGILQGIPVFDRKIMMIDIGGGSTEILVGHRGNILFSKSF
KLGAIRLTEKFFQEEPLSNSGIRKCRLFIEEMLLPFRKILRDLKPDLVVGSSGTIQAIAGMILASRGESEEISLNNFSFL
TSEFKKIRNLILEADTSRKRTKIPGLDSKRADIIIGGTLILEEIFDLANVPALTVSEFALREGIVYDTIRKWEHFESREH
SKHLDAIRQTSVDNFMRAFSRDEEYSKHVANLSLQIFDQLASLHKLGQEQRELLEAASLLHEIGQSISHSAYHKHSYYMI
RNSEMLLGFTFLEIEIIALTARYHRKNIPKLKHREFNKLAEKNRNLVCQLSAILRISSALNRNRGGLVPSVTCKTEKNQI
RFVLHTNKGYDPSLEIWAAEEQKIAFEETFGYSVEFVTGNVR

Sequences:

>Translated_522_residues
MVQEKPLAAIDLGTNSFHMIIVRVRTNGTFEAIAREKESVRLGNRLQENGPIDSDSFRRGIDCLKRFKILAENAGAEIRA
VATSALREASNREEFLEVVYQETGISIDVVSGYEEARLIYFGILQGIPVFDRKIMMIDIGGGSTEILVGHRGNILFSKSF
KLGAIRLTEKFFQEEPLSNSGIRKCRLFIEEMLLPFRKILRDLKPDLVVGSSGTIQAIAGMILASRGESEEISLNNFSFL
TSEFKKIRNLILEADTSRKRTKIPGLDSKRADIIIGGTLILEEIFDLANVPALTVSEFALREGIVYDTIRKWEHFESREH
SKHLDAIRQTSVDNFMRAFSRDEEYSKHVANLSLQIFDQLASLHKLGQEQRELLEAASLLHEIGQSISHSAYHKHSYYMI
RNSEMLLGFTFLEIEIIALTARYHRKNIPKLKHREFNKLAEKNRNLVCQLSAILRISSALNRNRGGLVPSVTCKTEKNQI
RFVLHTNKGYDPSLEIWAAEEQKIAFEETFGYSVEFVTGNVR
>Mature_522_residues
MVQEKPLAAIDLGTNSFHMIIVRVRTNGTFEAIAREKESVRLGNRLQENGPIDSDSFRRGIDCLKRFKILAENAGAEIRA
VATSALREASNREEFLEVVYQETGISIDVVSGYEEARLIYFGILQGIPVFDRKIMMIDIGGGSTEILVGHRGNILFSKSF
KLGAIRLTEKFFQEEPLSNSGIRKCRLFIEEMLLPFRKILRDLKPDLVVGSSGTIQAIAGMILASRGESEEISLNNFSFL
TSEFKKIRNLILEADTSRKRTKIPGLDSKRADIIIGGTLILEEIFDLANVPALTVSEFALREGIVYDTIRKWEHFESREH
SKHLDAIRQTSVDNFMRAFSRDEEYSKHVANLSLQIFDQLASLHKLGQEQRELLEAASLLHEIGQSISHSAYHKHSYYMI
RNSEMLLGFTFLEIEIIALTARYHRKNIPKLKHREFNKLAEKNRNLVCQLSAILRISSALNRNRGGLVPSVTCKTEKNQI
RFVLHTNKGYDPSLEIWAAEEQKIAFEETFGYSVEFVTGNVR

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=462, Percent_Identity=31.1688311688312, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI48994966, Length=481, Percent_Identity=30.7692307692308, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321185, Length=280, Percent_Identity=23.5714285714286, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 59271; Mature: 59271

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQEKPLAAIDLGTNSFHMIIVRVRTNGTFEAIAREKESVRLGNRLQENGPIDSDSFRRG
CCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IDCLKRFKILAENAGAEIRAVATSALREASNREEFLEVVYQETGISIDVVSGYEEARLIY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHE
FGILQGIPVFDRKIMMIDIGGGSTEILVGHRGNILFSKSFKLGAIRLTEKFFQEEPLSNS
EHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCC
GIRKCRLFIEEMLLPFRKILRDLKPDLVVGSSGTIQAIAGMILASRGESEEISLNNFSFL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCEEECCCHHHH
TSEFKKIRNLILEADTSRKRTKIPGLDSKRADIIIGGTLILEEIFDLANVPALTVSEFAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHH
REGIVYDTIRKWEHFESREHSKHLDAIRQTSVDNFMRAFSRDEEYSKHVANLSLQIFDQL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASLHKLGQEQRELLEAASLLHEIGQSISHSAYHKHSYYMIRNSEMLLGFTFLEIEIIALT
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEEEEEEEE
ARYHRKNIPKLKHREFNKLAEKNRNLVCQLSAILRISSALNRNRGGLVPSVTCKTEKNQI
HHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEE
RFVLHTNKGYDPSLEIWAAEEQKIAFEETFGYSVEFVTGNVR
EEEEECCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MVQEKPLAAIDLGTNSFHMIIVRVRTNGTFEAIAREKESVRLGNRLQENGPIDSDSFRRG
CCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IDCLKRFKILAENAGAEIRAVATSALREASNREEFLEVVYQETGISIDVVSGYEEARLIY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHE
FGILQGIPVFDRKIMMIDIGGGSTEILVGHRGNILFSKSFKLGAIRLTEKFFQEEPLSNS
EHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCCC
GIRKCRLFIEEMLLPFRKILRDLKPDLVVGSSGTIQAIAGMILASRGESEEISLNNFSFL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCEEECCCHHHH
TSEFKKIRNLILEADTSRKRTKIPGLDSKRADIIIGGTLILEEIFDLANVPALTVSEFAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHH
REGIVYDTIRKWEHFESREHSKHLDAIRQTSVDNFMRAFSRDEEYSKHVANLSLQIFDQL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASLHKLGQEQRELLEAASLLHEIGQSISHSAYHKHSYYMIRNSEMLLGFTFLEIEIIALT
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEEEEEEEE
ARYHRKNIPKLKHREFNKLAEKNRNLVCQLSAILRISSALNRNRGGLVPSVTCKTEKNQI
HHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEE
RFVLHTNKGYDPSLEIWAAEEQKIAFEETFGYSVEFVTGNVR
EEEEECCCCCCCCEEEEECCCCCEEHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10382967; 10984043 [H]