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The map label for this gene is sbp [H]

Identifier: 116328592

GI number: 116328592

Start: 2276124

End: 2277128

Strand: Direct

Name: sbp [H]

Synonym: LBL_1949

Alternate gene names: 116328592

Gene position: 2276124-2277128 (Clockwise)

Preceding gene: 116328591

Following gene: 116328594

Centisome position: 62.97

GC content: 43.78

Gene sequence:

>1005_bases
ATGAAAACAAAAATTCTTAAATCAATTCTAATTGCTCTTCTCATCACGAACGCGAACGCCGGACTTTTCTCAAAGGACGT
AAACCTGCTCAATGTATCTTACGACCCTACGAGAGAGCTCTACGCGGAATATAACAAACTTTTTGCAAGTTATTGGAAAT
CTAAAACGGGAGACGAGGTAAGAGTCAATCAATCCCACGGCGGAAGCGGCAAACAAGCAAGGTCGGTGATCGACGGACTC
GAAGCGGATATCGTCACCCTCGCACTCTCCTACGATATCGACATCATAGCTTCGAAAGGCCTGATCTCGAGGGATTGGTT
GAAAAGTCTTCCGAACAACAGCACTCCTTATACTTCTACGATCGTATTTGTAGTCAGAAAAGGAAATCCCAAGAATATAA
AAGACTGGGACGATCTAGTTCGATCTAAAGTCGGCGTCATAACACCGAATCCAAAAACGAGCGGAGGGGCACGCTGGAAT
TATCTAGCCGCTTGGGCTTTCGCTCAGAAAAAGTTCAATAACGATACGAATAAAGTTCAGGACTTTATAAAAACGCTATA
TAAAAACGTTCCCGTTCTCGACTCGGGAGCCAGAGGTTCTAGCAACACTTTCGCTCAAAACGGTATCGGAGATGTATTGA
TCGCTTGGGAAAACGAATCTTTTCTTATCTTAGAAGAATTTGGAAAAGATAAATACGAAGTTATTATTCCGTCGTTGAGT
ATTCTCGCAGAGCCACCCGTCGCAATCGTAGAAAAGAACGCGGAAAAACACGGCACCTTAGAGACGGCAAAGGCGTATCT
CAAGTCTCTCTATTCCGATGCGGCACAAGAAATCATCGCAAAACACGGATATCGTCCGAGAAATCAGACCATTCTCAAAA
AGCACGAAGGAAAATTTCCAAAATTGGACCTGATCACCGTGGATGGGCATTTCGGCGGATGGCATAAAGCACAAAAAGAT
CATTTTGCAGACGGAGCTTCTTTCGATCAACTCTACGTTAAGTAA

Upstream 100 bases:

>100_bases
GAACGTCAATGACAGATACACGCTGGACCATAAGTCCTACAGCTTCTTCTTGTTCGTCCAATTTGCAATGTAATCCAAAA
ATCAAAATTAAGGAATCAAT

Downstream 100 bases:

>100_bases
AAACGAAAAATATCTCCAAAAGAAAATGAAAACCTCGTATACCATTTTTCTATTGAAAAATAAAAGAATTCTTTTGGACA
AATAACGACCCCAAAGCCGA

Product: substrate binding protein of an ABC transporter complex

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKTKILKSILIALLITNANAGLFSKDVNLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALSYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVRSKVGVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVIIPSLS
ILAEPPVAIVEKNAEKHGTLETAKAYLKSLYSDAAQEIIAKHGYRPRNQTILKKHEGKFPKLDLITVDGHFGGWHKAQKD
HFADGASFDQLYVK

Sequences:

>Translated_334_residues
MKTKILKSILIALLITNANAGLFSKDVNLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALSYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVRSKVGVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVIIPSLS
ILAEPPVAIVEKNAEKHGTLETAKAYLKSLYSDAAQEIIAKHGYRPRNQTILKKHEGKFPKLDLITVDGHFGGWHKAQKD
HFADGASFDQLYVK
>Mature_334_residues
MKTKILKSILIALLITNANAGLFSKDVNLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALSYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVRSKVGVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVIIPSLS
ILAEPPVAIVEKNAEKHGTLETAKAYLKSLYSDAAQEIIAKHGYRPRNQTILKKHEGKFPKLDLITVDGHFGGWHKAQKD
HFADGASFDQLYVK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=321, Percent_Identity=63.5514018691589, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=49.1803278688525, Blast_Score=292, Evalue=3e-80,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37168; Mature: 37168

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKILKSILIALLITNANAGLFSKDVNLLNVSYDPTRELYAEYNKLFASYWKSKTGDEV
CCHHHHHHHHHHHHHCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
RVNQSHGGSGKQARSVIDGLEADIVTLALSYDIDIIASKGLISRDWLKSLPNNSTPYTST
EEECCCCCCCHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCHHHHHHCCCCCCCCEEE
IVFVVRKGNPKNIKDWDDLVRSKVGVITPNPKTSGGARWNYLAAWAFAQKKFNNDTNKVQ
EEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
DFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVIIPSLS
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCHH
ILAEPPVAIVEKNAEKHGTLETAKAYLKSLYSDAAQEIIAKHGYRPRNQTILKKHEGKFP
HHCCCCHHEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
KLDLITVDGHFGGWHKAQKDHFADGASFDQLYVK
CEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEC
>Mature Secondary Structure
MKTKILKSILIALLITNANAGLFSKDVNLLNVSYDPTRELYAEYNKLFASYWKSKTGDEV
CCHHHHHHHHHHHHHCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
RVNQSHGGSGKQARSVIDGLEADIVTLALSYDIDIIASKGLISRDWLKSLPNNSTPYTST
EEECCCCCCCHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCHHHHHHCCCCCCCCEEE
IVFVVRKGNPKNIKDWDDLVRSKVGVITPNPKTSGGARWNYLAAWAFAQKKFNNDTNKVQ
EEEEEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
DFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVIIPSLS
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCHH
ILAEPPVAIVEKNAEKHGTLETAKAYLKSLYSDAAQEIIAKHGYRPRNQTILKKHEGKFP
HHCCCCHHEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
KLDLITVDGHFGGWHKAQKDHFADGASFDQLYVK
CEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]