Click here to switch to the map view.

The map label for this gene is eno

Identifier: 116328443

GI number: 116328443

Start: 2077974

End: 2079272

Strand: Reverse

Name: eno

Synonym: LBL_1785

Alternate gene names: 116328443

Gene position: 2079272-2077974 (Counterclockwise)

Preceding gene: 116328450

Following gene: 116328442

Centisome position: 57.53

GC content: 44.96

Gene sequence:

>1299_bases
ATGTCTCATAACTCTCAAATTCAGAAAATTCAAGCTAGGGAAATTATCGACTCCCGAGGAAATCCAACAGTAGAAGTGGA
TGTAACACTTATGGACGGTTCTTTTGGTAGGGCGGCTGTTCCTTCCGGAGCATCTACCGGAGAATACGAAGCCGTCGAAC
TTAGAGACGGTGATAAACAGCGTTATCTCGGGAAAGGAGTTCTCAAAGCGGTAGAGCATGTAAACGTAAAAATTCAAGAA
ATACTAAAGGGTCAAGACGCCCTGGATCAAAACCGGGTCGATCAACTGATGCTCGACGCAGACGGGACCAAAAACAAAGG
TAAACTCGGAGCCAACGCGATTCTCGGCACTTCTCTTGCGGTAGCAAAAGCGGCTGCTTTTCATTCCAAACTTCCTTTAT
ATCGTTATATCGGCGGAAACTTTGCCCGTGAACTTCCGGTTCCTATGATGAACATTATTAACGGAGGAGCGCACGCGGAC
AACAATGTGGATTTTCAGGAGTTTATGATTCTTCCTGTGGGGGCCAAAAATTTTCGTGAGGGACTTAGAATGGGGGCCGA
AGTGTTCCATTCTTTAAAGTCGGTCCTCAAAGGTAAGAAATTGAACACCGCGGTTGGTGACGAAGGCGGCTTTGCTCCTG
ATCTTACGAGCAACGTGGAAGCGATCGAAGTCATTCTCCAGGCGATCGAAAAAGCAGGGTATAAACCGGAAAAAGACGTT
TTATTGGGTTTAGATGCGGCTTCTTCCGAGTTTTATGACAAAAGCAAAAAGAAATACGTACTCGGTGCCGAAAATAATAA
GGAGTTCTCCAGTGCAGAACTGGTGGATTATTATGCGAATCTCGTCTCCAAATATCCGATCATTACGATCGAAGACGGAC
TAGACGAGAATGATTGGGAAGGCTGGAAACTTCTTTCCGAAAAGTTGGGAAAAAAAATTCAGCTCGTGGGAGACGATCTT
TTTGTGACGAACATCGAGAAACTCTCCAAGGGAATAACTTCCGGAGTCGGGAATTCGATTCTCATCAAGGTGAATCAGAT
CGGTTCCCTCTCGGAAACTCTTGCGTCGATCGAAATGGCGAAAAAGGCGAAATACACGAATGTCGTGAGCCATAGAAGCG
GAGAAACGGAAGATGTTACGATTTCTCACATTGCAGTTGCGACTAATGCGGGGCAGATCAAGACAGGTTCTCTTTCTAGA
ACGGATCGAATCGCGAAATATAACGAACTTCTGAGAATCGAAGAAGAACTCGGAAAATCCGCGGTTTACAAAGGTAAGGA
AACTTTTTATAATCTATAA

Upstream 100 bases:

>100_bases
TGATTTCTTTGGATATGGACCGTTTCATAATCCTAACTCCGAACAAAATCGCCAATCGCGAACATTCAACGGAAAACATA
CATAAATCTCAGGAAACAAA

Downstream 100 bases:

>100_bases
GAATGTTTTCCGATTATTTGGACTGTAAGTTCTAAGGTCCATAGAAACTAAGAATTCGGTTCGAGTTTTTGTGTGAGAAT
CGATTTCGGGAAAAAGGAGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSHNSQIQKIQAREIIDSRGNPTVEVDVTLMDGSFGRAAVPSGASTGEYEAVELRDGDKQRYLGKGVLKAVEHVNVKIQE
ILKGQDALDQNRVDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAFHSKLPLYRYIGGNFARELPVPMMNIINGGAHAD
NNVDFQEFMILPVGAKNFREGLRMGAEVFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWEGWKLLSEKLGKKIQLVGDDL
FVTNIEKLSKGITSGVGNSILIKVNQIGSLSETLASIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSR
TDRIAKYNELLRIEEELGKSAVYKGKETFYNL

Sequences:

>Translated_432_residues
MSHNSQIQKIQAREIIDSRGNPTVEVDVTLMDGSFGRAAVPSGASTGEYEAVELRDGDKQRYLGKGVLKAVEHVNVKIQE
ILKGQDALDQNRVDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAFHSKLPLYRYIGGNFARELPVPMMNIINGGAHAD
NNVDFQEFMILPVGAKNFREGLRMGAEVFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWEGWKLLSEKLGKKIQLVGDDL
FVTNIEKLSKGITSGVGNSILIKVNQIGSLSETLASIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSR
TDRIAKYNELLRIEEELGKSAVYKGKETFYNL
>Mature_431_residues
SHNSQIQKIQAREIIDSRGNPTVEVDVTLMDGSFGRAAVPSGASTGEYEAVELRDGDKQRYLGKGVLKAVEHVNVKIQEI
LKGQDALDQNRVDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAFHSKLPLYRYIGGNFARELPVPMMNIINGGAHADN
NVDFQEFMILPVGAKNFREGLRMGAEVFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDVL
LGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWEGWKLLSEKLGKKIQLVGDDLF
VTNIEKLSKGITSGVGNSILIKVNQIGSLSETLASIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRT
DRIAKYNELLRIEEELGKSAVYKGKETFYNL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=52.1028037383178, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=51.981351981352, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=51.508120649652, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=51.508120649652, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=47.0862470862471, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=26.8361581920904, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=26.8361581920904, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=26.8361581920904, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=61.8266978922717, Blast_Score=509, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=51.0489510489511, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=51.0489510489511, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=41.7989417989418, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=47.3317865429234, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=47.0997679814385, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=47.0997679814385, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=46.8181818181818, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=46.8181818181818, Blast_Score=352, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=50.1144164759725, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=50.1144164759725, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=50.1144164759725, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=50.1144164759725, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=50.462962962963, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=50.462962962963, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_LEPBJ (Q04SI9)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800889.1
- ProteinModelPortal:   Q04SI9
- SMR:   Q04SI9
- STRING:   Q04SI9
- GeneID:   4411492
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1561
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q04SI9
- ProtClustDB:   PRK00077
- BioCyc:   LBOR355277:LBJ_1561-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47046; Mature: 46915

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 208-208 ACT_SITE 343-343 BINDING 158-158 BINDING 167-167 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNSQIQKIQAREIIDSRGNPTVEVDVTLMDGSFGRAAVPSGASTGEYEAVELRDGDKQ
CCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHH
RYLGKGVLKAVEHVNVKIQEILKGQDALDQNRVDQLMLDADGTKNKGKLGANAILGTSLA
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHH
VAKAAAFHSKLPLYRYIGGNFARELPVPMMNIINGGAHADNNVDFQEFMILPVGAKNFRE
HHHHHHHHHCCCHHHHHCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHH
GLRMGAEVFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWE
EEECCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
GWKLLSEKLGKKIQLVGDDLFVTNIEKLSKGITSGVGNSILIKVNQIGSLSETLASIEMA
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
KKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRTDRIAKYNELLRIEEELGKS
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCHH
AVYKGKETFYNL
HHHCCHHHHCCC
>Mature Secondary Structure 
SHNSQIQKIQAREIIDSRGNPTVEVDVTLMDGSFGRAAVPSGASTGEYEAVELRDGDKQ
CCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCHH
RYLGKGVLKAVEHVNVKIQEILKGQDALDQNRVDQLMLDADGTKNKGKLGANAILGTSLA
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHH
VAKAAAFHSKLPLYRYIGGNFARELPVPMMNIINGGAHADNNVDFQEFMILPVGAKNFRE
HHHHHHHHHCCCHHHHHCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHH
GLRMGAEVFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWE
EEECCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
GWKLLSEKLGKKIQLVGDDLFVTNIEKLSKGITSGVGNSILIKVNQIGSLSETLASIEMA
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
KKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRTDRIAKYNELLRIEEELGKS
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCHH
AVYKGKETFYNL
HHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA