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The map label for this gene is cca [H]

Identifier: 116328419

GI number: 116328419

Start: 2052310

End: 2053608

Strand: Reverse

Name: cca [H]

Synonym: LBL_1760

Alternate gene names: 116328419

Gene position: 2053608-2052310 (Counterclockwise)

Preceding gene: 116328420

Following gene: 116328414

Centisome position: 56.82

GC content: 41.42

Gene sequence:

>1299_bases
ATGACTAACGTGGATCCGGACGAATTAATCAAAAAAATTCCCGAAAACTTTCGGGAAGATATAATTCGGATCACACAAAA
CATCCGCAAAGAGGGCGGAGAATGTTATCTGATCGGCGGATCCGTGCGCGATTTGTTGCTTTCAAAAATACCGGACGAAT
TCGATTTGACCACTTCACTGTTGCCAGACAAAATTCTTACCTTATTCAAAAGAACGGTTCCGACCGGGATCAAACATGGC
ACAGTTACCGTTTTAATACAAGACCGTTCTTACGAGATCACTACCTTCCGAAAGGACGTGGACTATATAGACGGGAGAAG
ACCGGAGGCCGTTGAATTCGGAGTTTCGTTAAGCGAGGATTTAAAACGAAGAGATTTTACTATGAATGCTTTGGCGTTGG
ACTTGGAAGCGAAACGCCTGATCGACGAGCATTCTGGACTTGAGGACATTCAAAATAAGATCATTCGAACGATCGGAGAT
CCGATCGAAAGATTTACCGAAGACGGTCTTCGTCCAATCCGTGCGATTCGTTTCGTAAGTAGTCTCGGTTTCGCTTTGGA
GTCGGAAACCGCAAAGGCGATTATCCAATGTAGAAATATAACCGCGAAAATTTCTAAAGAAAGGGTTCACGACGAATTAA
ACAAAACCCTCAAAAGCAAAAATCCGGCTCCTTCTTTGAAGTTATTCAAGGAGTTTCGAATTTTAGAATTATTTACGAGT
CTCAAATTGTATCCGACGGAAAATACGAACGTAGAAAAAAAAATCCGGGAAGTTCCTGTGGTTCCTTTGAGCCTTAGGCT
TTCTTTTTTACATGCTTGGCTGTTCGGAAGTTTTCAAGATAGAAATTCGGCAAAACAGTTTATGAAAGATCTCCGATATT
CCAATCAAAACACGAAGGATTCACTGTTTTTCTCGGATATGCTTTTTATGATGTTGAACCGGAAGGATGACGCGTCTTTG
ACCGATGCGGAAATTCGAAAGGCTGTTTTGCATCCGATTTGTGTGTATGCAGGAAGAGAAAATCTAAGGATTTTGACGGA
ATATATTCTTAATCTTGCATATACTTACGAGCCCATTCTCAAGGATATTTTCGATTTTCGGAGAATTCTGGATCTGACCG
AACGATCTCAAGCTCTTGTTGTGACTGAATTGGCGCTTCGAGGAGAGGATATCCTCAGGGCTTGTCCGAATCTTCCTCCT
AAACAAATAGGATCCGTCCTTGAGAAACTACTGTTTCATGTTCTGGAAAATCCGGATGAAAATCGAAAAGATTTCCTTTT
TCCTCTTCTTTTCAAGTAA

Upstream 100 bases:

>100_bases
ACGGTGGATCCGGAATGGAAAAAGAAAATCGATATCATTCTTGAAAATAACCGAACTCTCAAAAATCGATACGGGAATCT
CTGGAAAAAAATGATTCAAA

Downstream 100 bases:

>100_bases
CTCATTCTATTCCTATCGAGCGATTCATAAAGAGCGAGATAGTCGTAGCCAGCGAAAGCATTACCGAGCGACCCTAGAAG
CGAGGTTGAGTTATTCGAAG

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNVDPDELIKKIPENFREDIIRITQNIRKEGGECYLIGGSVRDLLLSKIPDEFDLTTSLLPDKILTLFKRTVPTGIKHG
TVTVLIQDRSYEITTFRKDVDYIDGRRPEAVEFGVSLSEDLKRRDFTMNALALDLEAKRLIDEHSGLEDIQNKIIRTIGD
PIERFTEDGLRPIRAIRFVSSLGFALESETAKAIIQCRNITAKISKERVHDELNKTLKSKNPAPSLKLFKEFRILELFTS
LKLYPTENTNVEKKIREVPVVPLSLRLSFLHAWLFGSFQDRNSAKQFMKDLRYSNQNTKDSLFFSDMLFMMLNRKDDASL
TDAEIRKAVLHPICVYAGRENLRILTEYILNLAYTYEPILKDIFDFRRILDLTERSQALVVTELALRGEDILRACPNLPP
KQIGSVLEKLLFHVLENPDENRKDFLFPLLFK

Sequences:

>Translated_432_residues
MTNVDPDELIKKIPENFREDIIRITQNIRKEGGECYLIGGSVRDLLLSKIPDEFDLTTSLLPDKILTLFKRTVPTGIKHG
TVTVLIQDRSYEITTFRKDVDYIDGRRPEAVEFGVSLSEDLKRRDFTMNALALDLEAKRLIDEHSGLEDIQNKIIRTIGD
PIERFTEDGLRPIRAIRFVSSLGFALESETAKAIIQCRNITAKISKERVHDELNKTLKSKNPAPSLKLFKEFRILELFTS
LKLYPTENTNVEKKIREVPVVPLSLRLSFLHAWLFGSFQDRNSAKQFMKDLRYSNQNTKDSLFFSDMLFMMLNRKDDASL
TDAEIRKAVLHPICVYAGRENLRILTEYILNLAYTYEPILKDIFDFRRILDLTERSQALVVTELALRGEDILRACPNLPP
KQIGSVLEKLLFHVLENPDENRKDFLFPLLFK
>Mature_431_residues
TNVDPDELIKKIPENFREDIIRITQNIRKEGGECYLIGGSVRDLLLSKIPDEFDLTTSLLPDKILTLFKRTVPTGIKHGT
VTVLIQDRSYEITTFRKDVDYIDGRRPEAVEFGVSLSEDLKRRDFTMNALALDLEAKRLIDEHSGLEDIQNKIIRTIGDP
IERFTEDGLRPIRAIRFVSSLGFALESETAKAIIQCRNITAKISKERVHDELNKTLKSKNPAPSLKLFKEFRILELFTSL
KLYPTENTNVEKKIREVPVVPLSLRLSFLHAWLFGSFQDRNSAKQFMKDLRYSNQNTKDSLFFSDMLFMMLNRKDDASLT
DAEIRKAVLHPICVYAGRENLRILTEYILNLAYTYEPILKDIFDFRRILDLTERSQALVVTELALRGEDILRACPNLPPK
QIGSVLEKLLFHVLENPDENRKDFLFPLLFK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=36.3636363636364, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI87081691, Length=253, Percent_Identity=31.2252964426877, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789436, Length=197, Percent_Identity=31.4720812182741, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=224, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281360112, Length=219, Percent_Identity=32.4200913242009, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357337, Length=219, Percent_Identity=32.4200913242009, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 49909; Mature: 49778

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVDPDELIKKIPENFREDIIRITQNIRKEGGECYLIGGSVRDLLLSKIPDEFDLTTSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHCHHHHH
LPDKILTLFKRTVPTGIKHGTVTVLIQDRSYEITTFRKDVDYIDGRRPEAVEFGVSLSED
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEHHHHHHHHCCCCCCHHHHCCCHHHH
LKRRDFTMNALALDLEAKRLIDEHSGLEDIQNKIIRTIGDPIERFTEDGLRPIRAIRFVS
HHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SLGFALESETAKAIIQCRNITAKISKERVHDELNKTLKSKNPAPSLKLFKEFRILELFTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LKLYPTENTNVEKKIREVPVVPLSLRLSFLHAWLFGSFQDRNSAKQFMKDLRYSNQNTKD
HEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCH
SLFFSDMLFMMLNRKDDASLTDAEIRKAVLHPICVYAGRENLRILTEYILNLAYTYEPIL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KDIFDFRRILDLTERSQALVVTELALRGEDILRACPNLPPKQIGSVLEKLLFHVLENPDE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NRKDFLFPLLFK
CCHHCCCHHHCC
>Mature Secondary Structure 
TNVDPDELIKKIPENFREDIIRITQNIRKEGGECYLIGGSVRDLLLSKIPDEFDLTTSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHCHHHHH
LPDKILTLFKRTVPTGIKHGTVTVLIQDRSYEITTFRKDVDYIDGRRPEAVEFGVSLSED
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEHHHHHHHHCCCCCCHHHHCCCHHHH
LKRRDFTMNALALDLEAKRLIDEHSGLEDIQNKIIRTIGDPIERFTEDGLRPIRAIRFVS
HHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SLGFALESETAKAIIQCRNITAKISKERVHDELNKTLKSKNPAPSLKLFKEFRILELFTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LKLYPTENTNVEKKIREVPVVPLSLRLSFLHAWLFGSFQDRNSAKQFMKDLRYSNQNTKD
HEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCH
SLFFSDMLFMMLNRKDDASLTDAEIRKAVLHPICVYAGRENLRILTEYILNLAYTYEPIL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KDIFDFRRILDLTERSQALVVTELALRGEDILRACPNLPPKQIGSVLEKLLFHVLENPDE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NRKDFLFPLLFK
CCHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]