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The map label for this gene is map [C]

Identifier: 116328345

GI number: 116328345

Start: 1960686

End: 1961444

Strand: Reverse

Name: map [C]

Synonym: LBL_1682

Alternate gene names: 116328345

Gene position: 1961444-1960686 (Counterclockwise)

Preceding gene: 116328346

Following gene: 116328344

Centisome position: 54.27

GC content: 42.69

Gene sequence:

>759_bases
TTGATCTTCATTAAAAATAAGAGCGAAATCGAAAAAATGAGAGCGGCTGGGAAACTGGCCGCTAGACTTTTGGATTATAT
TTCCTCCTATGTTCAGCCGGGTGTTTCCACTCTCCAGTTGAATGATCTCAGCGAAGAGTTTACGAAAAAACACGGAGCCA
AATCCGCTCCGCTCGGTTATAAAGGTTTTCCTAAATCGATTTGCACTTCCGTAAATCAAGTTGTTTGTCACGGGATTCCT
AAAGCAACAGAAATTTTGAAAGAAGGAGATATCGTTAACATTGATGTGACTCCTCTTTTGAATGGTTATCACGGGGATTC
ATCACGTACGTTTATCGTCGGTGGAAAGACGAGTCCCGAAGTGGAGGCTCTCGTTCAAGATACGGAAAAAGCGATGTATA
TCGGGATTGAACAAGTTAAACCCGGAAATCGTGTGCATGATATTGCAAATGCGATCGATGATTTCCTAACTCCCAAAGGA
TACGGGATCGTAAGAGATTTGATGGGACACGGAATCGGAAGAGGATTTCACGAAGATCCGCAAATTCCTCATTTTCGTCA
GAATCGAAAACTCGCCAAGTTGGAGCCGGGAATGATCTTTACAGTCGAACCGATGGTAAATCTTGGAACATGGCGGGTGA
ATTTTTCCAAAGAGGATCATTGGACCGTAACGACTATAGACGGTAAGTGGTCCGCGCAATTCGAACATACTGTTCTAGTT
ACTGAAAAAGGCTATGAAATTTTAACCGTTTCCGATTAA

Upstream 100 bases:

>100_bases
CGGTTTGAACGAGAAGATTCACCAACTGATCGATGAAGCAGTGAAACGTACTGAATCGAATAAGAGAAGTACTGTTCGCC
CAACTGATTTCTAATTCGAT

Downstream 100 bases:

>100_bases
TCTGGTGTTATGGAAACGGTACTTGGTTATCTAAGCGCTTTAGGTAGAGACGCGGTATTTTTTTTAATCAGTTTTGTTCT
CTTTTACATCGGTAAAAAAA

Product: methionyl aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIFIKNKSEIEKMRAAGKLAARLLDYISSYVQPGVSTLQLNDLSEEFTKKHGAKSAPLGYKGFPKSICTSVNQVVCHGIP
KATEILKEGDIVNIDVTPLLNGYHGDSSRTFIVGGKTSPEVEALVQDTEKAMYIGIEQVKPGNRVHDIANAIDDFLTPKG
YGIVRDLMGHGIGRGFHEDPQIPHFRQNRKLAKLEPGMIFTVEPMVNLGTWRVNFSKEDHWTVTTIDGKWSAQFEHTVLV
TEKGYEILTVSD

Sequences:

>Translated_252_residues
MIFIKNKSEIEKMRAAGKLAARLLDYISSYVQPGVSTLQLNDLSEEFTKKHGAKSAPLGYKGFPKSICTSVNQVVCHGIP
KATEILKEGDIVNIDVTPLLNGYHGDSSRTFIVGGKTSPEVEALVQDTEKAMYIGIEQVKPGNRVHDIANAIDDFLTPKG
YGIVRDLMGHGIGRGFHEDPQIPHFRQNRKLAKLEPGMIFTVEPMVNLGTWRVNFSKEDHWTVTTIDGKWSAQFEHTVLV
TEKGYEILTVSD
>Mature_252_residues
MIFIKNKSEIEKMRAAGKLAARLLDYISSYVQPGVSTLQLNDLSEEFTKKHGAKSAPLGYKGFPKSICTSVNQVVCHGIP
KATEILKEGDIVNIDVTPLLNGYHGDSSRTFIVGGKTSPEVEALVQDTEKAMYIGIEQVKPGNRVHDIANAIDDFLTPKG
YGIVRDLMGHGIGRGFHEDPQIPHFRQNRKLAKLEPGMIFTVEPMVNLGTWRVNFSKEDHWTVTTIDGKWSAQFEHTVLV
TEKGYEILTVSD

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=248, Percent_Identity=42.3387096774194, Blast_Score=204, Evalue=4e-53,
Organism=Homo sapiens, GI40385867, Length=253, Percent_Identity=40.3162055335968, Blast_Score=177, Evalue=9e-45,
Organism=Escherichia coli, GI1786364, Length=250, Percent_Identity=49.6, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1788728, Length=251, Percent_Identity=30.2788844621514, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71996291, Length=252, Percent_Identity=40.4761904761905, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323273, Length=251, Percent_Identity=44.6215139442231, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21355531, Length=248, Percent_Identity=43.9516129032258, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=42.6829268292683, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27982; Mature: 27982

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFIKNKSEIEKMRAAGKLAARLLDYISSYVQPGVSTLQLNDLSEEFTKKHGAKSAPLGY
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCC
KGFPKSICTSVNQVVCHGIPKATEILKEGDIVNIDVTPLLNGYHGDSSRTFIVGGKTSPE
CCCCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHCCCCCCCCCCCCEEEECCCCCHH
VEALVQDTEKAMYIGIEQVKPGNRVHDIANAIDDFLTPKGYGIVRDLMGHGIGRGFHEDP
HHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
QIPHFRQNRKLAKLEPGMIFTVEPMVNLGTWRVNFSKEDHWTVTTIDGKWSAQFEHTVLV
CCCCHHCCCCEEEECCCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEE
TEKGYEILTVSD
EECCEEEEEECC
>Mature Secondary Structure
MIFIKNKSEIEKMRAAGKLAARLLDYISSYVQPGVSTLQLNDLSEEFTKKHGAKSAPLGY
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCC
KGFPKSICTSVNQVVCHGIPKATEILKEGDIVNIDVTPLLNGYHGDSSRTFIVGGKTSPE
CCCCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHCCCCCCCCCCCCEEEECCCCCHH
VEALVQDTEKAMYIGIEQVKPGNRVHDIANAIDDFLTPKGYGIVRDLMGHGIGRGFHEDP
HHHHHHHHHHEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
QIPHFRQNRKLAKLEPGMIFTVEPMVNLGTWRVNFSKEDHWTVTTIDGKWSAQFEHTVLV
CCCCHHCCCCEEEECCCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEE
TEKGYEILTVSD
EECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]