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The map label for this gene is pyrG [H]

Identifier: 116328338

GI number: 116328338

Start: 1954359

End: 1955981

Strand: Reverse

Name: pyrG [H]

Synonym: LBL_1675

Alternate gene names: 116328338

Gene position: 1955981-1954359 (Counterclockwise)

Preceding gene: 116328339

Following gene: 116328337

Centisome position: 54.12

GC content: 43.25

Gene sequence:

>1623_bases
TTGTCGAGAACTAAGTTTATCTTTGTCACAGGAGGGGTAAGTTCCTCACTTGGAAAAGGGGTCACAGTTGCGGCTCTGGG
TTGTTTATTGGAAAGTAGAGGATACACGGTCTCTCTCCAGAAAATGGACCCTTATATCAATATCGATCCGGGAACCATGA
GTCCCTATCAACATGGAGAAGTTTACGTAACCGCAGATGGAGCGGAAACCGATTTGGATCTCGGTTACTACGAACGTTTC
ACTCATTCCAAACTGTCCCGCAAAAATTCCGTATCCACCGGTCAAATTTATAATACCGTCATTCAAAGAGAAAGGAAGGG
GGATTACCTGGGTCGTACAGTTCAGGTAGTTCCTCATATTACGAATGAAATCCGAAATCGGATGTACATCGTCGCCAGAG
AGGAAAATCCGGATTTTATCATCGTGGAGATTGGTGGGACCGTGGGCGACATCGAATCGATTCCGTTCTTAGAAGCGATT
CGCCAGATACGTTACGAACACGGAAGTTCGAACGTGTTATTCGTGCATCTTACTTTGGTTCCAACGATTACCGCTGCCGG
GGAAGCGAAGACAAAACCGACACAACATTCGGTCAAAGAATTACTCGGGCTTGGAATTCAACCGGATATCCTAGTGTGTA
GGGTTTCTCAGCCCATGACGAAAGAGATGAAGAACAAACTATCTCTTTTTTGTAACGTAAAAGAGGAAAACGTAATCTCT
GCGAGTGATATATCCACATCCATCTATGAAATTCCTAAAATGTATAAGGAAGAAAAGTTAGACGAAGTAGTTCTCAAAAC
GATGGGAATGGAACTTAGGGAATCCAATTTTTCCGAATGGGATAAGATGATAAGGAGACTACTCGCCACAAAACAAACCG
TTCAAGTAGCGATTGTGGGAAAATACATCTCTTTACAAGACGCATATCGTTCCATCTATGAAAGTCTTTCCCATGGCGGA
ATCGCTTACGATACCAAAGTGGAATTCGTCAAAATCGATCCTGAAAATTTAAATAAAGACAATGTCCAAAGTTCTTTGAA
GAACGTTCACGGGGTTTTAGTCCCGGGTGGCTTCGGAGATCGTGGAATCGAAGGAAAAATTCTCGCGATCGAGTATGCAA
GAACGAATGGGATTCCTTTTTTCGGAATCTGTCTTGGAATGCAATGTGCCGTCGTAGAATATGGAAGAAACGTTTTGAAA
CTCAAAGACGCCAATTCAACCGAAATCAGGCCGGACACGGAATATCCGGTGATATCGCTTTTGGAAGAACAAAACGACAT
CGAACAGATGGGCGCAACGATGAGGCTCGGTTCTTATCCTTGTAAAATTAAAAAAGATACTCTTTCGTACTCCGAATACA
AATCGACACTCGTCCACGAACGTCATAGGCATAGATTCGAATTCACCAACCGTTATAGAAAGCAATATGAAGAAAACGGA
ATGGTGATTTCGGGTACTTCTCCGGACGACAGCCTCGTAGAAATCGTGGAAATTCCGAAACACAATTGGTTTGTAGGGGT
TCAGTTTCATCCCGAATTTCAATCCAGACCTACAAAGCCTCATCCCTTATTTGCTGGATTTATCCGTGCGGCCGTGAAAT
ACTCAAAGAAAGGATCATCATGA

Upstream 100 bases:

>100_bases
ACGTATCGTCCCATTTTTTTAGAGACAGAATTCTTGGAAATTCCGGTTTGAATTTCAAGAAACACTAAAAAACTCTGTTC
TTAAAAGGCGGGAGTACAAG

Downstream 100 bases:

>100_bases
GAGACAATACCTGTACTAAAAGAGATTTTCTAAACGGAAGTAAGATTGGAGGAGACGAACCTTTCTTTTTGATCGCCGGT
CCCTGTGTGATGGAGAATCG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSRTKFIFVTGGVSSSLGKGVTVAALGCLLESRGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTADGAETDLDLGYYERF
THSKLSRKNSVSTGQIYNTVIQRERKGDYLGRTVQVVPHITNEIRNRMYIVAREENPDFIIVEIGGTVGDIESIPFLEAI
RQIRYEHGSSNVLFVHLTLVPTITAAGEAKTKPTQHSVKELLGLGIQPDILVCRVSQPMTKEMKNKLSLFCNVKEENVIS
ASDISTSIYEIPKMYKEEKLDEVVLKTMGMELRESNFSEWDKMIRRLLATKQTVQVAIVGKYISLQDAYRSIYESLSHGG
IAYDTKVEFVKIDPENLNKDNVQSSLKNVHGVLVPGGFGDRGIEGKILAIEYARTNGIPFFGICLGMQCAVVEYGRNVLK
LKDANSTEIRPDTEYPVISLLEEQNDIEQMGATMRLGSYPCKIKKDTLSYSEYKSTLVHERHRHRFEFTNRYRKQYEENG
MVISGTSPDDSLVEIVEIPKHNWFVGVQFHPEFQSRPTKPHPLFAGFIRAAVKYSKKGSS

Sequences:

>Translated_540_residues
MSRTKFIFVTGGVSSSLGKGVTVAALGCLLESRGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTADGAETDLDLGYYERF
THSKLSRKNSVSTGQIYNTVIQRERKGDYLGRTVQVVPHITNEIRNRMYIVAREENPDFIIVEIGGTVGDIESIPFLEAI
RQIRYEHGSSNVLFVHLTLVPTITAAGEAKTKPTQHSVKELLGLGIQPDILVCRVSQPMTKEMKNKLSLFCNVKEENVIS
ASDISTSIYEIPKMYKEEKLDEVVLKTMGMELRESNFSEWDKMIRRLLATKQTVQVAIVGKYISLQDAYRSIYESLSHGG
IAYDTKVEFVKIDPENLNKDNVQSSLKNVHGVLVPGGFGDRGIEGKILAIEYARTNGIPFFGICLGMQCAVVEYGRNVLK
LKDANSTEIRPDTEYPVISLLEEQNDIEQMGATMRLGSYPCKIKKDTLSYSEYKSTLVHERHRHRFEFTNRYRKQYEENG
MVISGTSPDDSLVEIVEIPKHNWFVGVQFHPEFQSRPTKPHPLFAGFIRAAVKYSKKGSS
>Mature_539_residues
SRTKFIFVTGGVSSSLGKGVTVAALGCLLESRGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTADGAETDLDLGYYERFT
HSKLSRKNSVSTGQIYNTVIQRERKGDYLGRTVQVVPHITNEIRNRMYIVAREENPDFIIVEIGGTVGDIESIPFLEAIR
QIRYEHGSSNVLFVHLTLVPTITAAGEAKTKPTQHSVKELLGLGIQPDILVCRVSQPMTKEMKNKLSLFCNVKEENVISA
SDISTSIYEIPKMYKEEKLDEVVLKTMGMELRESNFSEWDKMIRRLLATKQTVQVAIVGKYISLQDAYRSIYESLSHGGI
AYDTKVEFVKIDPENLNKDNVQSSLKNVHGVLVPGGFGDRGIEGKILAIEYARTNGIPFFGICLGMQCAVVEYGRNVLKL
KDANSTEIRPDTEYPVISLLEEQNDIEQMGATMRLGSYPCKIKKDTLSYSEYKSTLVHERHRHRFEFTNRYRKQYEENGM
VISGTSPDDSLVEIVEIPKHNWFVGVQFHPEFQSRPTKPHPLFAGFIRAAVKYSKKGSS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=45.3571428571429, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=43.978102189781, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=43.978102189781, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=43.978102189781, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=52.0220588235294, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25148299, Length=605, Percent_Identity=36.5289256198347, Blast_Score=397, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=562, Percent_Identity=40.7473309608541, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=39.9644760213144, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24664469, Length=554, Percent_Identity=45.6678700361011, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI21357815, Length=510, Percent_Identity=43.5294117647059, Blast_Score=408, Evalue=1e-114,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60815; Mature: 60684

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTKFIFVTGGVSSSLGKGVTVAALGCLLESRGYTVSLQKMDPYINIDPGTMSPYQHGE
CCCEEEEEEECCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCE
VYVTADGAETDLDLGYYERFTHSKLSRKNSVSTGQIYNTVIQRERKGDYLGRTVQVVPHI
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHH
TNEIRNRMYIVAREENPDFIIVEIGGTVGDIESIPFLEAIRQIRYEHGSSNVLFVHLTLV
HHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PTITAAGEAKTKPTQHSVKELLGLGIQPDILVCRVSQPMTKEMKNKLSLFCNVKEENVIS
EEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHEEEEEECCCCCEEE
ASDISTSIYEIPKMYKEEKLDEVVLKTMGMELRESNFSEWDKMIRRLLATKQTVQVAIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEE
KYISLQDAYRSIYESLSHGGIAYDTKVEFVKIDPENLNKDNVQSSLKNVHGVLVPGGFGD
CHHHHHHHHHHHHHHHHCCCEEEECEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCC
RGIEGKILAIEYARTNGIPFFGICLGMQCAVVEYGRNVLKLKDANSTEIRPDTEYPVISL
CCCCCEEEEEEEECCCCCCEEHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHH
LEEQNDIEQMGATMRLGSYPCKIKKDTLSYSEYKSTLVHERHRHRFEFTNRYRKQYEENG
HHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MVISGTSPDDSLVEIVEIPKHNWFVGVQFHPEFQSRPTKPHPLFAGFIRAAVKYSKKGSS
EEEECCCCCHHHHHHEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRTKFIFVTGGVSSSLGKGVTVAALGCLLESRGYTVSLQKMDPYINIDPGTMSPYQHGE
CCEEEEEEECCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCE
VYVTADGAETDLDLGYYERFTHSKLSRKNSVSTGQIYNTVIQRERKGDYLGRTVQVVPHI
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHH
TNEIRNRMYIVAREENPDFIIVEIGGTVGDIESIPFLEAIRQIRYEHGSSNVLFVHLTLV
HHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEE
PTITAAGEAKTKPTQHSVKELLGLGIQPDILVCRVSQPMTKEMKNKLSLFCNVKEENVIS
EEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHEEEEEECCCCCEEE
ASDISTSIYEIPKMYKEEKLDEVVLKTMGMELRESNFSEWDKMIRRLLATKQTVQVAIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCCEEEEEEEE
KYISLQDAYRSIYESLSHGGIAYDTKVEFVKIDPENLNKDNVQSSLKNVHGVLVPGGFGD
CHHHHHHHHHHHHHHHHCCCEEEECEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCC
RGIEGKILAIEYARTNGIPFFGICLGMQCAVVEYGRNVLKLKDANSTEIRPDTEYPVISL
CCCCCEEEEEEEECCCCCCEEHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHH
LEEQNDIEQMGATMRLGSYPCKIKKDTLSYSEYKSTLVHERHRHRFEFTNRYRKQYEENG
HHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MVISGTSPDDSLVEIVEIPKHNWFVGVQFHPEFQSRPTKPHPLFAGFIRAAVKYSKKGSS
EEEECCCCCHHHHHHEECCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA