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The map label for this gene is rnhB [H]

Identifier: 116328318

GI number: 116328318

Start: 1936678

End: 1937349

Strand: Reverse

Name: rnhB [H]

Synonym: LBL_1655

Alternate gene names: 116328318

Gene position: 1937349-1936678 (Counterclockwise)

Preceding gene: 116328319

Following gene: 116328317

Centisome position: 53.6

GC content: 40.18

Gene sequence:

>672_bases
TTGCCGGAGTCGTTTTTTACTCATTTTGAACCGGAAGAATACCGGTTTTATTCAGAGTCGATTCCTTGTGGAATCGACGA
AGCCGGAAGAGGTCCTTATGCAGGACCTCTTTCTGTTGCATTGGTTTCTTTTTCTCAAAATACGCTTCACCAAATCCTGG
AAGGCAAACTTCTTAAAGGATTAACGGATTCTAAAAAACTTTCCGAGAAAAAGCGAGAAGCACTTTATCCGGAAATTTTA
AAAATCGCACAAATTTCGTGCCAAACCTTCTTAAGCCCGAATTATATCGATCGGGAAGGGATCAATCGAGCTGTACTAGA
AGGAATTCGAAAATGCGCTAAAATGGCGATTCGGGGTGTTTCATCCACTCTTCCACTGCAACTTTTAATCGACGGAAATT
ACAACTTCAATCGTTACCCAGAATGGAATTATTTCAAGAATCGTTCCTCTTTTTATACAAAAGGAGATCTTAGAATCGTG
AGCATTGCAGCCGCCTCTATTTTAGCAAAAGTCAGTCGGGATCGTTATATGACTTCCATCTCTAAAAAATATCCCGATTA
TCGATTCGACCGACATAAGGGATACGGAACAAAACTGCACGAAGAACTGATTCTCAGGTATGGCCTTTCCGATATTCATA
GAAGAAGTTTTACCGGAAAATTTCTACAGTGA

Upstream 100 bases:

>100_bases
CTCGCATCAAAGAACTCAAAGGCAGTAAAGCCCTTGTAAGTGAAGATAGAAAAAGACAACAAGAGACTGCTGTAAAATCA
AAAGCAGAATAAGCCGGGTT

Downstream 100 bases:

>100_bases
TTCCGGTCTAATGATTAAGCAGGACGACCAAAAATATTTTGGAAGTTTTGAATTTATAAGATGTCCATTATGATTCTTTT
CGAAATCAGGTTCTTATGAA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MPESFFTHFEPEEYRFYSESIPCGIDEAGRGPYAGPLSVALVSFSQNTLHQILEGKLLKGLTDSKKLSEKKREALYPEIL
KIAQISCQTFLSPNYIDREGINRAVLEGIRKCAKMAIRGVSSTLPLQLLIDGNYNFNRYPEWNYFKNRSSFYTKGDLRIV
SIAAASILAKVSRDRYMTSISKKYPDYRFDRHKGYGTKLHEELILRYGLSDIHRRSFTGKFLQ

Sequences:

>Translated_223_residues
MPESFFTHFEPEEYRFYSESIPCGIDEAGRGPYAGPLSVALVSFSQNTLHQILEGKLLKGLTDSKKLSEKKREALYPEIL
KIAQISCQTFLSPNYIDREGINRAVLEGIRKCAKMAIRGVSSTLPLQLLIDGNYNFNRYPEWNYFKNRSSFYTKGDLRIV
SIAAASILAKVSRDRYMTSISKKYPDYRFDRHKGYGTKLHEELILRYGLSDIHRRSFTGKFLQ
>Mature_222_residues
PESFFTHFEPEEYRFYSESIPCGIDEAGRGPYAGPLSVALVSFSQNTLHQILEGKLLKGLTDSKKLSEKKREALYPEILK
IAQISCQTFLSPNYIDREGINRAVLEGIRKCAKMAIRGVSSTLPLQLLIDGNYNFNRYPEWNYFKNRSSFYTKGDLRIVS
IAAASILAKVSRDRYMTSISKKYPDYRFDRHKGYGTKLHEELILRYGLSDIHRRSFTGKFLQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=205, Percent_Identity=36.5853658536585, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 25672; Mature: 25541

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESFFTHFEPEEYRFYSESIPCGIDEAGRGPYAGPLSVALVSFSQNTLHQILEGKLLKG
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHC
LTDSKKLSEKKREALYPEILKIAQISCQTFLSPNYIDREGINRAVLEGIRKCAKMAIRGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHCC
SSTLPLQLLIDGNYNFNRYPEWNYFKNRSSFYTKGDLRIVSIAAASILAKVSRDRYMTSI
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHH
SKKYPDYRFDRHKGYGTKLHEELILRYGLSDIHRRSFTGKFLQ
HHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PESFFTHFEPEEYRFYSESIPCGIDEAGRGPYAGPLSVALVSFSQNTLHQILEGKLLKG
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHC
LTDSKKLSEKKREALYPEILKIAQISCQTFLSPNYIDREGINRAVLEGIRKCAKMAIRGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHCC
SSTLPLQLLIDGNYNFNRYPEWNYFKNRSSFYTKGDLRIVSIAAASILAKVSRDRYMTSI
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHH
SKKYPDYRFDRHKGYGTKLHEELILRYGLSDIHRRSFTGKFLQ
HHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA