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The map label for this gene is tyrS [H]

Identifier: 116328275

GI number: 116328275

Start: 1881889

End: 1883106

Strand: Direct

Name: tyrS [H]

Synonym: LBL_1606

Alternate gene names: 116328275

Gene position: 1881889-1883106 (Clockwise)

Preceding gene: 116328273

Following gene: 116328276

Centisome position: 52.07

GC content: 39.9

Gene sequence:

>1218_bases
ATGGACATTCAAAAGCAAATAGAAATCATCCGTCGTGGTACCGTTGATCTGATCAGCGAAGACGAATTAAAATCCAAGCT
TCAAAAAAAGAAAGCACTTAAAATTAAGGCCGGTTTCGATCCTACGGCTCCCGATCTTCATCTCGGACATTTCGTCCAAC
TTAAAAAATTAAAACATTTCCAAGACTTAGGTCATGAAGTTTTTTTTCTACTTGGGGATTTTACGGCAATGATCGGAGAT
CCCACCGGGAAATCCGAAACTAGAAAGAGGCTTTCGAAGGAAGAGGTTTTGGAAAACTCTAAAACTTATCAAAGTCAGGT
TTTTAAGGTCTTAGATCCCGCTAAAACAAGAATCGTCTACAATTCAAGTTGGTGTTCCGGGATGAACTTCGAAGACGTCT
TGGTTCTCAGTTCCAAATATAACGTTGCAAGAATGCTCGAAAGGGACGATTTCAACAAACGTTATAAAGCCGGTCATCCG
ATTTCCATGATCGAATTTTTATACCCGCTCGTCCAAGGTTATGATTCCGTAGCGATGGAATGCGACGTAGAACTTGGAGG
AACGGATCAAAAATTTAACCTTCTCGTGGGAAGAGACTTACAAAGAGAATACGGGAAGGAAGTTCAATGTGTTCTTACGC
TTCCGTTACTTGTGGGATTGGACGGAAATAAAAAAATGTCTAAATCCCTCGGTAACTACGTGGGAATCACCGAAGTTCCG
ATCGACATGTTCGGAAAACTAATGTCGATCAGTGACGATCTGATGTGGAATTATTTTGAACTCTTAACCGATCTTCCGTT
GTCAGAAATCGAAGTTCGTAAAAACGGAATAGTAAACAAAGAACTTCATCCGAAAGAAGTGAAGATAGAACTTGCAAAGC
TCATAATGGACCAACTTTCTTCTCCTTCCGAAAACGAAGAAGCGATAGAAGAATGGAAAAAAATCCATAATCCAAAATCG
AGGGCCATTCCCGACGATATCAGAGAAGTAAAACTTGGGGAAGAATTCTTTTCGGAAACTCCGGAACCCTTGCTCGTCTG
GGTTTTAAGTAAACTTTCTTTCGTTCCTTCCGTTTCCGAAGGAAGAAGACTCATCAAGGCAGGAGGGTTATATCTTTTTG
AGGATAAAATCACCGATGAAAAATTCGCGATCCAAAAAGATAAGGAATACTTAATAAGGCAGGGAAAAAAGGGAAAATTT
TTGAAAATTCTTTCCTAA

Upstream 100 bases:

>100_bases
GGTTTTGTTTTTCCATTTTGAGAACTCTTCCTGCTTTACAGGTTTCTTTCCCCCTACATATTTGCATTAAGTTTCACAAT
CCAATCCGGATTTTTCTTTC

Downstream 100 bases:

>100_bases
AAATGATTTGAAGCTTTTGAAATTTTTCATTTCAATCAAATCCTCATGTTAAGCGAAGAAGAATCGTCGGAACTTTCGAA
GACAATCTCCGAAATTAAAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDIQKQIEIIRRGTVDLISEDELKSKLQKKKALKIKAGFDPTAPDLHLGHFVQLKKLKHFQDLGHEVFFLLGDFTAMIGD
PTGKSETRKRLSKEEVLENSKTYQSQVFKVLDPAKTRIVYNSSWCSGMNFEDVLVLSSKYNVARMLERDDFNKRYKAGHP
ISMIEFLYPLVQGYDSVAMECDVELGGTDQKFNLLVGRDLQREYGKEVQCVLTLPLLVGLDGNKKMSKSLGNYVGITEVP
IDMFGKLMSISDDLMWNYFELLTDLPLSEIEVRKNGIVNKELHPKEVKIELAKLIMDQLSSPSENEEAIEEWKKIHNPKS
RAIPDDIREVKLGEEFFSETPEPLLVWVLSKLSFVPSVSEGRRLIKAGGLYLFEDKITDEKFAIQKDKEYLIRQGKKGKF
LKILS

Sequences:

>Translated_405_residues
MDIQKQIEIIRRGTVDLISEDELKSKLQKKKALKIKAGFDPTAPDLHLGHFVQLKKLKHFQDLGHEVFFLLGDFTAMIGD
PTGKSETRKRLSKEEVLENSKTYQSQVFKVLDPAKTRIVYNSSWCSGMNFEDVLVLSSKYNVARMLERDDFNKRYKAGHP
ISMIEFLYPLVQGYDSVAMECDVELGGTDQKFNLLVGRDLQREYGKEVQCVLTLPLLVGLDGNKKMSKSLGNYVGITEVP
IDMFGKLMSISDDLMWNYFELLTDLPLSEIEVRKNGIVNKELHPKEVKIELAKLIMDQLSSPSENEEAIEEWKKIHNPKS
RAIPDDIREVKLGEEFFSETPEPLLVWVLSKLSFVPSVSEGRRLIKAGGLYLFEDKITDEKFAIQKDKEYLIRQGKKGKF
LKILS
>Mature_405_residues
MDIQKQIEIIRRGTVDLISEDELKSKLQKKKALKIKAGFDPTAPDLHLGHFVQLKKLKHFQDLGHEVFFLLGDFTAMIGD
PTGKSETRKRLSKEEVLENSKTYQSQVFKVLDPAKTRIVYNSSWCSGMNFEDVLVLSSKYNVARMLERDDFNKRYKAGHP
ISMIEFLYPLVQGYDSVAMECDVELGGTDQKFNLLVGRDLQREYGKEVQCVLTLPLLVGLDGNKKMSKSLGNYVGITEVP
IDMFGKLMSISDDLMWNYFELLTDLPLSEIEVRKNGIVNKELHPKEVKIELAKLIMDQLSSPSENEEAIEEWKKIHNPKS
RAIPDDIREVKLGEEFFSETPEPLLVWVLSKLSFVPSVSEGRRLIKAGGLYLFEDKITDEKFAIQKDKEYLIRQGKKGKF
LKILS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=377, Percent_Identity=26.790450928382, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=26.1904761904762, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25145802, Length=281, Percent_Identity=28.8256227758007, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=26.530612244898, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130383, Length=446, Percent_Identity=25.1121076233184, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46357; Mature: 46357

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQKQIEIIRRGTVDLISEDELKSKLQKKKALKIKAGFDPTAPDLHLGHFVQLKKLKHF
CCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHH
QDLGHEVFFLLGDFTAMIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKVLDPAKTRIVY
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHEEEEE
NSSWCSGMNFEDVLVLSSKYNVARMLERDDFNKRYKAGHPISMIEFLYPLVQGYDSVAME
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEE
CDVELGGTDQKFNLLVGRDLQREYGKEVQCVLTLPLLVGLDGNKKMSKSLGNYVGITEVP
EEEEECCCCCEEEEEECCHHHHHCCCCEEEEEEEEHHEECCCCHHHHHHHCCCCCCCCCC
IDMFGKLMSISDDLMWNYFELLTDLPLSEIEVRKNGIVNKELHPKEVKIELAKLIMDQLS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
SPSENEEAIEEWKKIHNPKSRAIPDDIREVKLGEEFFSETPEPLLVWVLSKLSFVPSVSE
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCHHH
GRRLIKAGGLYLFEDKITDEKFAIQKDKEYLIRQGKKGKFLKILS
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MDIQKQIEIIRRGTVDLISEDELKSKLQKKKALKIKAGFDPTAPDLHLGHFVQLKKLKHF
CCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHH
QDLGHEVFFLLGDFTAMIGDPTGKSETRKRLSKEEVLENSKTYQSQVFKVLDPAKTRIVY
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHEEEEE
NSSWCSGMNFEDVLVLSSKYNVARMLERDDFNKRYKAGHPISMIEFLYPLVQGYDSVAME
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEE
CDVELGGTDQKFNLLVGRDLQREYGKEVQCVLTLPLLVGLDGNKKMSKSLGNYVGITEVP
EEEEECCCCCEEEEEECCHHHHHCCCCEEEEEEEEHHEECCCCHHHHHHHCCCCCCCCCC
IDMFGKLMSISDDLMWNYFELLTDLPLSEIEVRKNGIVNKELHPKEVKIELAKLIMDQLS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
SPSENEEAIEEWKKIHNPKSRAIPDDIREVKLGEEFFSETPEPLLVWVLSKLSFVPSVSE
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHCCCHHH
GRRLIKAGGLYLFEDKITDEKFAIQKDKEYLIRQGKKGKFLKILS
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA