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The map label for this gene is dnaG [H]

Identifier: 116328272

GI number: 116328272

Start: 1874061

End: 1875857

Strand: Direct

Name: dnaG [H]

Synonym: LBL_1600

Alternate gene names: 116328272

Gene position: 1874061-1875857 (Clockwise)

Preceding gene: 116328271

Following gene: 116328273

Centisome position: 51.85

GC content: 39.57

Gene sequence:

>1797_bases
TTGTCTAACAAAAAGGATTTCATCGATCGAATTCACCGGGAAGTCCCCATTGAGTCCTATATTTCTCGTTTTGTTCCGCT
TAAAAAACGAGGAAAGAATTTTTTAGGACTTTGTCCATTTCACCAAGAAAAATCTCCTTCTTTTAACGTTTCCGCAGAGA
AACAGTTCTATTACTGTTTTGGCTGTAAGGCTTCGGGTGATCTCATCCGATTTGTGATGGATTATGAGAGAGTGGATTTT
TCCAGATCACTGGAAATTCTTTCCGAATACTCCGGAATCCCTCTGCAAGAAAAAAATTCTAAAAACGCAGAAATCTCCGA
CTTTCTTTATAAGATCAATCAAAAGGTTTCCGAATATTATCAACATTTATTGCATACTCCGATCGGGAAGAATGCTTTTG
ATTATTTAAAATCCAGAGAAATCGAAGATTCTGAAATCCGTATTTTTGGTCTTGGTTATGCGCCGGAAGGATTTGAAACA
CTTATGAAAGAAGTTTTAAAAACCAAAGATGAAATTGCCGGAGCAATTCAGTTGGGACTTCTTCGGGAAAAAGAAGCGGG
TAATGGACGTCCTTATGATTTTTTCCGCGATCGAATCATGTTTCCGGTCTTGGATTTATCCGGAAGAACGATCGCATTCT
CCGGGAGAATTTTAGGTCCTGGAAAGGAAGCAAAATACATCAATAGTCCTGCTTCCATAATCTTTGATAAGAGTCGTACT
TTTTATAATTTTTTTAGAGCTAGAGAGGGAGTACGTAAGACCGGAGAAGCTATACTCGTCGAAGGATATTTGGACGTGAT
CGGGCTTGTAAGAAGAGATTATGAAAACGTGATCGCTTCGATGGGAACTGCCATTACGGAGAATCATATTCGAACTCTTA
AAAAATTTGCAGAACGGATTACTTTCGTTTTGGACGGGGACATAGCAGGAAAAAAAGGCGCTCTGAGAGCGGCTGAAATC
TGTCTTAAGGAAGGAATGGAATGTTCCATCGTTCTCCTTCCCGAAGGAAAAGATCCATTCGACTTGGCAAAATCTTTGAG
TAGACCTCAATTGAACAAACTTTTGTCGGAACAAATTCAAGGTTCCGAATTCGTAATAGGGGAATTGCTGGAAAGTGCGG
ATTCACGTGCGTTACCTGAAAGAAAGAGAAAAGCTCTTCAAAATCTTTATTCTTTTACTCAAACTTTAAATCGGGAAACG
GATAAACAGTTTTTTCTGGGGCTTGGTGCGAACAAACTCGGAATCAGTATGGACGCGGTTCTTCGGGATTTTAAAGGAGG
AACAAGTGCAAAGAATGGGCCTGTGAATGCCGATATTAGTTCTAAACTAAAGGAAGTTCAAGAGATCTCAGCTCCGGCGT
TGGATTGTGAGAGAAAAATCATCTCTATGCTGGTCAAACATACGGGACTTTTTTCTTACGCAGAAGAAATCTCTTCGATG
GAATTCATGGATGTAACGAGTTCCTATCTTTGGGATTACTTGTATACCGTTTATACAGGAGAAGGCGAGATTTCTCCTGT
ACAGATTTTAGCCTCGGAACTTCCCGAGGATTTGAAACAAATACTTGCTCCGTATCTCTTAGAAGAATACGAAAAAGAAG
AGCCGGAAGAATTACATAAGGTTTTTCGACTTCTTTTGTTGCAACAAAAGAAGTTTCGGATTGAGGAAAGAATTCGGGAA
CTCGATCAAAAAAGAGAACGTTTTTTTACACCCGAAATCTTTACGGAACTGAGTTTCTATCGTAAAGAAAAAGAAAAGAT
CCTGGAGCATATCCGGAATCAATCGGCTACCACATAA

Upstream 100 bases:

>100_bases
GGGTCGAGTCATGGCCGCATTCAAAGGAAAAAGCATCGACGGTACTAAAGTTTCGGCAATTGTGAAATCCAGGCTTTCCT
GAATCTTAAGTGAGCTCCCT

Downstream 100 bases:

>100_bases
TTTAGAGGGAATGGAATGGAAAATCTGCAAAGCATGCCTGAGGTTCAGAAGATTATATCTCTTGGAAAGGCAAACGGAGA
AGTTTCTTATGATGATATCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MSNKKDFIDRIHREVPIESYISRFVPLKKRGKNFLGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMDYERVDF
SRSLEILSEYSGIPLQEKNSKNAEISDFLYKINQKVSEYYQHLLHTPIGKNAFDYLKSREIEDSEIRIFGLGYAPEGFET
LMKEVLKTKDEIAGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTIAFSGRILGPGKEAKYINSPASIIFDKSRT
FYNFFRAREGVRKTGEAILVEGYLDVIGLVRRDYENVIASMGTAITENHIRTLKKFAERITFVLDGDIAGKKGALRAAEI
CLKEGMECSIVLLPEGKDPFDLAKSLSRPQLNKLLSEQIQGSEFVIGELLESADSRALPERKRKALQNLYSFTQTLNRET
DKQFFLGLGANKLGISMDAVLRDFKGGTSAKNGPVNADISSKLKEVQEISAPALDCERKIISMLVKHTGLFSYAEEISSM
EFMDVTSSYLWDYLYTVYTGEGEISPVQILASELPEDLKQILAPYLLEEYEKEEPEELHKVFRLLLLQQKKFRIEERIRE
LDQKRERFFTPEIFTELSFYRKEKEKILEHIRNQSATT

Sequences:

>Translated_598_residues
MSNKKDFIDRIHREVPIESYISRFVPLKKRGKNFLGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMDYERVDF
SRSLEILSEYSGIPLQEKNSKNAEISDFLYKINQKVSEYYQHLLHTPIGKNAFDYLKSREIEDSEIRIFGLGYAPEGFET
LMKEVLKTKDEIAGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTIAFSGRILGPGKEAKYINSPASIIFDKSRT
FYNFFRAREGVRKTGEAILVEGYLDVIGLVRRDYENVIASMGTAITENHIRTLKKFAERITFVLDGDIAGKKGALRAAEI
CLKEGMECSIVLLPEGKDPFDLAKSLSRPQLNKLLSEQIQGSEFVIGELLESADSRALPERKRKALQNLYSFTQTLNRET
DKQFFLGLGANKLGISMDAVLRDFKGGTSAKNGPVNADISSKLKEVQEISAPALDCERKIISMLVKHTGLFSYAEEISSM
EFMDVTSSYLWDYLYTVYTGEGEISPVQILASELPEDLKQILAPYLLEEYEKEEPEELHKVFRLLLLQQKKFRIEERIRE
LDQKRERFFTPEIFTELSFYRKEKEKILEHIRNQSATT
>Mature_597_residues
SNKKDFIDRIHREVPIESYISRFVPLKKRGKNFLGLCPFHQEKSPSFNVSAEKQFYYCFGCKASGDLIRFVMDYERVDFS
RSLEILSEYSGIPLQEKNSKNAEISDFLYKINQKVSEYYQHLLHTPIGKNAFDYLKSREIEDSEIRIFGLGYAPEGFETL
MKEVLKTKDEIAGAIQLGLLREKEAGNGRPYDFFRDRIMFPVLDLSGRTIAFSGRILGPGKEAKYINSPASIIFDKSRTF
YNFFRAREGVRKTGEAILVEGYLDVIGLVRRDYENVIASMGTAITENHIRTLKKFAERITFVLDGDIAGKKGALRAAEIC
LKEGMECSIVLLPEGKDPFDLAKSLSRPQLNKLLSEQIQGSEFVIGELLESADSRALPERKRKALQNLYSFTQTLNRETD
KQFFLGLGANKLGISMDAVLRDFKGGTSAKNGPVNADISSKLKEVQEISAPALDCERKIISMLVKHTGLFSYAEEISSME
FMDVTSSYLWDYLYTVYTGEGEISPVQILASELPEDLKQILAPYLLEEYEKEEPEELHKVFRLLLLQQKKFRIEERIREL
DQKRERFFTPEIFTELSFYRKEKEKILEHIRNQSATT

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=494, Percent_Identity=31.1740890688259, Blast_Score=245, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68602; Mature: 68471

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKDFIDRIHREVPIESYISRFVPLKKRGKNFLGLCPFHQEKSPSFNVSAEKQFYYCF
CCCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEE
GCKASGDLIRFVMDYERVDFSRSLEILSEYSGIPLQEKNSKNAEISDFLYKINQKVSEYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QHLLHTPIGKNAFDYLKSREIEDSEIRIFGLGYAPEGFETLMKEVLKTKDEIAGAIQLGL
HHHHCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LREKEAGNGRPYDFFRDRIMFPVLDLSGRTIAFSGRILGPGKEAKYINSPASIIFDKSRT
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCCCCCCCCHHEEEECCHH
FYNFFRAREGVRKTGEAILVEGYLDVIGLVRRDYENVIASMGTAITENHIRTLKKFAERI
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVLDGDIAGKKGALRAAEICLKEGMECSIVLLPEGKDPFDLAKSLSRPQLNKLLSEQIQ
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHC
GSEFVIGELLESADSRALPERKRKALQNLYSFTQTLNRETDKQFFLGLGANKLGISMDAV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHCCHHHHH
LRDFKGGTSAKNGPVNADISSKLKEVQEISAPALDCERKIISMLVKHTGLFSYAEEISSM
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EFMDVTSSYLWDYLYTVYTGEGEISPVQILASELPEDLKQILAPYLLEEYEKEEPEELHK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
VFRLLLLQQKKFRIEERIRELDQKRERFFTPEIFTELSFYRKEKEKILEHIRNQSATT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNKKDFIDRIHREVPIESYISRFVPLKKRGKNFLGLCPFHQEKSPSFNVSAEKQFYYCF
CCHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEEE
GCKASGDLIRFVMDYERVDFSRSLEILSEYSGIPLQEKNSKNAEISDFLYKINQKVSEYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QHLLHTPIGKNAFDYLKSREIEDSEIRIFGLGYAPEGFETLMKEVLKTKDEIAGAIQLGL
HHHHCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LREKEAGNGRPYDFFRDRIMFPVLDLSGRTIAFSGRILGPGKEAKYINSPASIIFDKSRT
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECEEECCCCCCCCCCCCHHEEEECCHH
FYNFFRAREGVRKTGEAILVEGYLDVIGLVRRDYENVIASMGTAITENHIRTLKKFAERI
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVLDGDIAGKKGALRAAEICLKEGMECSIVLLPEGKDPFDLAKSLSRPQLNKLLSEQIQ
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHC
GSEFVIGELLESADSRALPERKRKALQNLYSFTQTLNRETDKQFFLGLGANKLGISMDAV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHCCHHHHH
LRDFKGGTSAKNGPVNADISSKLKEVQEISAPALDCERKIISMLVKHTGLFSYAEEISSM
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EFMDVTSSYLWDYLYTVYTGEGEISPVQILASELPEDLKQILAPYLLEEYEKEEPEELHK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
VFRLLLLQQKKFRIEERIRELDQKRERFFTPEIFTELSFYRKEKEKILEHIRNQSATT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]