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The map label for this gene is 116328258

Identifier: 116328258

GI number: 116328258

Start: 1859411

End: 1859977

Strand: Direct

Name: 116328258

Synonym: LBL_1586

Alternate gene names: NA

Gene position: 1859411-1859977 (Clockwise)

Preceding gene: 116328251

Following gene: 116328260

Centisome position: 51.44

GC content: 41.45

Gene sequence:

>567_bases
ATGATTCAAACCGACTTGCATATAAAACAGGATTTTCTGACTCCAAATCCACATAGCAGACCGGGGGATAAAATAAAGGA
AGTGAAAGGGATCGTGTTACATTGGACGGCTTTACCGAATGCCACGGCTCAGAACATTCGCGATTATTTTGAAAGTTTGA
AAGCTCCGGAAGGTCGTTTTGCAAGTGCACATTTTGCAGTAGGGCTCGTTGGGGAGATTGTACAATGTATTCCTCTAGAT
GAAATCGCATATCATTGTGGTAGTAGAACTTACACTTCTGAAAAAGAAAAACGTTTAGGTCCGGATAGTCCTAATTTTTA
TACGATTGGGATTGAACAGTGTGTTCAGGATATGACGGGAAAGTTTACTGAAAAAACTCTAGATAGCGCGGCTAGACTGA
CCGCTCAGCTATGCCTTAAATACAATTTAGACGTAAACGACCTGTGGACCCATCAAGGTATAGTCGGATGGAAAGATTGT
CCTCGCTGGTACAACACTCATCCGAAGGACTGGATCGGCTTTAAAAAAAGAGTGGAAGATCTATTAAAAAGACAACGTAA
GGATTAG

Upstream 100 bases:

>100_bases
TTTTTTGTTAAAATATTATAAATATTCAAAACATAAAACTTGTTGTCGGTTCGTTTATGGTCTATAATTTATTATAATTC
CAATTATAGAGAGGTTATGC

Downstream 100 bases:

>100_bases
AATACAATAATTCGCGCGGATAAGTCTTTGCCGCATGGTCGCGATTAGATTGTTTTGTTTTGGATCCCAAAAGTAAGTTT
TTGAATATTCTAAAAAATTA

Product: amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase; ORFL3 [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MIQTDLHIKQDFLTPNPHSRPGDKIKEVKGIVLHWTALPNATAQNIRDYFESLKAPEGRFASAHFAVGLVGEIVQCIPLD
EIAYHCGSRTYTSEKEKRLGPDSPNFYTIGIEQCVQDMTGKFTEKTLDSAARLTAQLCLKYNLDVNDLWTHQGIVGWKDC
PRWYNTHPKDWIGFKKRVEDLLKRQRKD

Sequences:

>Translated_188_residues
MIQTDLHIKQDFLTPNPHSRPGDKIKEVKGIVLHWTALPNATAQNIRDYFESLKAPEGRFASAHFAVGLVGEIVQCIPLD
EIAYHCGSRTYTSEKEKRLGPDSPNFYTIGIEQCVQDMTGKFTEKTLDSAARLTAQLCLKYNLDVNDLWTHQGIVGWKDC
PRWYNTHPKDWIGFKKRVEDLLKRQRKD
>Mature_188_residues
MIQTDLHIKQDFLTPNPHSRPGDKIKEVKGIVLHWTALPNATAQNIRDYFESLKAPEGRFASAHFAVGLVGEIVQCIPLD
EIAYHCGSRTYTSEKEKRLGPDSPNFYTIGIEQCVQDMTGKFTEKTLDSAARLTAQLCLKYNLDVNDLWTHQGIVGWKDC
PRWYNTHPKDWIGFKKRVEDLLKRQRKD

Specific function: Unknown

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21586; Mature: 21586

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTDLHIKQDFLTPNPHSRPGDKIKEVKGIVLHWTALPNATAQNIRDYFESLKAPEGRF
CCCCCCCHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ASAHFAVGLVGEIVQCIPLDEIAYHCGSRTYTSEKEKRLGPDSPNFYTIGIEQCVQDMTG
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEECHHHHHHHHHH
KFTEKTLDSAARLTAQLCLKYNLDVNDLWTHQGIVGWKDCPRWYNTHPKDWIGFKKRVED
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHH
LLKRQRKD
HHHHHCCC
>Mature Secondary Structure
MIQTDLHIKQDFLTPNPHSRPGDKIKEVKGIVLHWTALPNATAQNIRDYFESLKAPEGRF
CCCCCCCHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ASAHFAVGLVGEIVQCIPLDEIAYHCGSRTYTSEKEKRLGPDSPNFYTIGIEQCVQDMTG
CHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEECHHHHHHHHHH
KFTEKTLDSAARLTAQLCLKYNLDVNDLWTHQGIVGWKDCPRWYNTHPKDWIGFKKRVED
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHH
LLKRQRKD
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2033382 [H]