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The map label for this gene is uvrD-2 [H]

Identifier: 116328254

GI number: 116328254

Start: 1850947

End: 1852944

Strand: Reverse

Name: uvrD-2 [H]

Synonym: LBL_1581

Alternate gene names: 116328254

Gene position: 1852944-1850947 (Counterclockwise)

Preceding gene: 116328255

Following gene: 116328253

Centisome position: 51.26

GC content: 39.99

Gene sequence:

>1998_bases
GTGTCCTGGAAAGAAGAATTAAATGCCGCTCAATTGGAAGCAGTCCTTACCCAGGAGGGACCGGTGCTTGTTTTAGCAGG
TGCCGGAACCGGTAAAACAAAAACCATCGTCAGTCGATTGGCGCAATTAGTTTCCTCGGGCGTTCCTACCTCATCCATTC
TACTTCTGACATTTACTCGCAAGGCCGCCAGAGAAATGCTTCTTAGAGCGTCTTCTATCGGAGACAAAAGGTGTACCGAG
GTTCAAGGTGGAACTTTTCATTCTTTTTGTAGCGGAGTCCTACGAAAATTTGCCCCGGTTCTTGGACTATCCTCCGGTTT
TACGATTTTGGACGAATCGGACACCTTAGACGTGTTCCAATTTCTAAGAAACGAAAAAGATTTCGGCAAAACCAAATCCC
GTTTTCCTTCCAATGAAACTTTAATTTCAATTTACGGTGAAATTCAAAACACTGGAAGAGTTCTTCAAACTGTATTAGAA
AAAGATTATCCTTTGTTTTTACAAAGAACGAAAGACATCTCCCAAATTTTTGAAGATTATAAATCGTATAAAACAGAACG
ATCCCTTTTGGATTACGACGACCTACTCTATTTTACTAGAGATCTTCTTACCAATCACCCGGGGGTCAGAAACGCACTTT
CCGAAAAATACAAATTTATCATGGTGGATGAATTTCAAGATACGAATAAGGTCCAAGCACATATAGCCTGTCTCCTAGCT
TCGGAGCATTCCAATTTGATGGTGGTCGGAGACGACGCTCAATGTATCTACACATTTCGCGGGGCTTCCGTGCGCGGTAT
TTTAGATTTTCCTAAAATATTTTCGAATACAAAAACGATTTTTTTGGAAAAAAATTATAGAAGTACAACCGCGATCTTAA
ACCTCGCAAACGCAGTCCTTCAAAACTTTTCCGAAAAATACGATAAATACCTTTTTACGGATAACGAAAACGGATCGATT
CCGAATGTTCTACAATTCGAAGACGAATTAGAGGAAGCCGAAGGAATCGCCGAAATTCTTCTACAAAAAAAAGAAGATGG
GATTCCCTTTAAAAAAATGTGTGTCCTTTTCCGCGCCGGATGGAACTCGAATCAGCTCGAACTTGTTCTCGCCAAACGGA
ATATTCCATTCGTAAAATTCGGCGGAAGGAAGTTTATAGAAACAGCTCACATCAAAGATCTGCTTTCCTTTTTAAAGGTG
CTCGTCAATCCCTTGGACTCGGTTTCTTGGATTCGAGTTTTAAAATTGATCCCCGGAATTGGAAATTCTAAAGCGAACGG
TATTTTAGATAAGATCCGGAAATCTTCCGGTTCTTTCGAAGTTCTTTTAGGAGAAAACGAAACCGCAATCGATAAATATA
TTTCCCCCCTTTATCATCTCTATCAAAAACACAGAGAAACCTATTCGGAAGTAAAAAAGGTCACTTCGGATTTTATCGAT
TACTACCGAGTTCTTTTGGAAAAAAATTACGACGATTCCAAACGAAGATCTGAAGATTTAGACTCTGTTCTAGGATTTTC
TTTGAAATACACCTCGCTCGGGGATTTTTTATCGGACCTTACGATGGATCACGCGTCCCTAAGTTTGGACAAAATCAAAC
CGGATGATGCCGAAACCGATCTTCTTAATTTATCCACCGTACATTCCGCAAAGGGATTGGAGTTCGATCTCGTTTTCATT
TTAAATGCAACCGAAGGAGTTTTTCCCTCGAGCAAGAACACCGACACCGAAGAAGAAAGAAGACTTTTTTACGTGGCGAT
TACAAGAGCCAGAAAAGAGTTATATTTTACAAGACCTTCTTTGGCTCATTCCAGATCCGGGCCATATTATACAAAACTAT
CCCGTTTTTTGAGTGAGATTCAATCTCCAGAAAAAGTCTACGAATTAAAACTTATACCTGGAAAATCAGTGCCGCAAAAT
TTCTTTTCGACGAAAGTCTCTTCCTCTAAAACCGCAAACGACTCTTTTTCCAAAATACAAGACTACTTCGGGAATTAA

Upstream 100 bases:

>100_bases
TCTTTGACGTAGAATTGACTGGAATTCGTGAAGCTACGGAAGAAGAAATCTCACACGGGCACGTTCACGGAGAAGATGGT
CACCACCACCATTGAAAGTT

Downstream 100 bases:

>100_bases
AATTCATGTCTTCCCTTTCCGAAAAATCAGAAGAAGAATCGATAAAGTCCACTGCACTGAATTTACTGGAAAAAAGAACA
TTCGAATTTCGTAAACTTCC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSWKEELNAAQLEAVLTQEGPVLVLAGAGTGKTKTIVSRLAQLVSSGVPTSSILLLTFTRKAAREMLLRASSIGDKRCTE
VQGGTFHSFCSGVLRKFAPVLGLSSGFTILDESDTLDVFQFLRNEKDFGKTKSRFPSNETLISIYGEIQNTGRVLQTVLE
KDYPLFLQRTKDISQIFEDYKSYKTERSLLDYDDLLYFTRDLLTNHPGVRNALSEKYKFIMVDEFQDTNKVQAHIACLLA
SEHSNLMVVGDDAQCIYTFRGASVRGILDFPKIFSNTKTIFLEKNYRSTTAILNLANAVLQNFSEKYDKYLFTDNENGSI
PNVLQFEDELEEAEGIAEILLQKKEDGIPFKKMCVLFRAGWNSNQLELVLAKRNIPFVKFGGRKFIETAHIKDLLSFLKV
LVNPLDSVSWIRVLKLIPGIGNSKANGILDKIRKSSGSFEVLLGENETAIDKYISPLYHLYQKHRETYSEVKKVTSDFID
YYRVLLEKNYDDSKRRSEDLDSVLGFSLKYTSLGDFLSDLTMDHASLSLDKIKPDDAETDLLNLSTVHSAKGLEFDLVFI
LNATEGVFPSSKNTDTEEERRLFYVAITRARKELYFTRPSLAHSRSGPYYTKLSRFLSEIQSPEKVYELKLIPGKSVPQN
FFSTKVSSSKTANDSFSKIQDYFGN

Sequences:

>Translated_665_residues
MSWKEELNAAQLEAVLTQEGPVLVLAGAGTGKTKTIVSRLAQLVSSGVPTSSILLLTFTRKAAREMLLRASSIGDKRCTE
VQGGTFHSFCSGVLRKFAPVLGLSSGFTILDESDTLDVFQFLRNEKDFGKTKSRFPSNETLISIYGEIQNTGRVLQTVLE
KDYPLFLQRTKDISQIFEDYKSYKTERSLLDYDDLLYFTRDLLTNHPGVRNALSEKYKFIMVDEFQDTNKVQAHIACLLA
SEHSNLMVVGDDAQCIYTFRGASVRGILDFPKIFSNTKTIFLEKNYRSTTAILNLANAVLQNFSEKYDKYLFTDNENGSI
PNVLQFEDELEEAEGIAEILLQKKEDGIPFKKMCVLFRAGWNSNQLELVLAKRNIPFVKFGGRKFIETAHIKDLLSFLKV
LVNPLDSVSWIRVLKLIPGIGNSKANGILDKIRKSSGSFEVLLGENETAIDKYISPLYHLYQKHRETYSEVKKVTSDFID
YYRVLLEKNYDDSKRRSEDLDSVLGFSLKYTSLGDFLSDLTMDHASLSLDKIKPDDAETDLLNLSTVHSAKGLEFDLVFI
LNATEGVFPSSKNTDTEEERRLFYVAITRARKELYFTRPSLAHSRSGPYYTKLSRFLSEIQSPEKVYELKLIPGKSVPQN
FFSTKVSSSKTANDSFSKIQDYFGN
>Mature_664_residues
SWKEELNAAQLEAVLTQEGPVLVLAGAGTGKTKTIVSRLAQLVSSGVPTSSILLLTFTRKAAREMLLRASSIGDKRCTEV
QGGTFHSFCSGVLRKFAPVLGLSSGFTILDESDTLDVFQFLRNEKDFGKTKSRFPSNETLISIYGEIQNTGRVLQTVLEK
DYPLFLQRTKDISQIFEDYKSYKTERSLLDYDDLLYFTRDLLTNHPGVRNALSEKYKFIMVDEFQDTNKVQAHIACLLAS
EHSNLMVVGDDAQCIYTFRGASVRGILDFPKIFSNTKTIFLEKNYRSTTAILNLANAVLQNFSEKYDKYLFTDNENGSIP
NVLQFEDELEEAEGIAEILLQKKEDGIPFKKMCVLFRAGWNSNQLELVLAKRNIPFVKFGGRKFIETAHIKDLLSFLKVL
VNPLDSVSWIRVLKLIPGIGNSKANGILDKIRKSSGSFEVLLGENETAIDKYISPLYHLYQKHRETYSEVKKVTSDFIDY
YRVLLEKNYDDSKRRSEDLDSVLGFSLKYTSLGDFLSDLTMDHASLSLDKIKPDDAETDLLNLSTVHSAKGLEFDLVFIL
NATEGVFPSSKNTDTEEERRLFYVAITRARKELYFTRPSLAHSRSGPYYTKLSRFLSEIQSPEKVYELKLIPGKSVPQNF
FSTKVSSSKTANDSFSKIQDYFGN

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=695, Percent_Identity=30.5035971223022, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=29.1666666666667, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6322369, Length=475, Percent_Identity=28.8421052631579, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324477, Length=663, Percent_Identity=23.2277526395173, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75264; Mature: 75132

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWKEELNAAQLEAVLTQEGPVLVLAGAGTGKTKTIVSRLAQLVSSGVPTSSILLLTFTR
CCCHHHCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECH
KAAREMLLRASSIGDKRCTEVQGGTFHSFCSGVLRKFAPVLGLSSGFTILDESDTLDVFQ
HHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHH
FLRNEKDFGKTKSRFPSNETLISIYGEIQNTGRVLQTVLEKDYPLFLQRTKDISQIFEDY
HHHCCCCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KSYKTERSLLDYDDLLYFTRDLLTNHPGVRNALSEKYKFIMVDEFQDTNKVQAHIACLLA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHEEE
SEHSNLMVVGDDAQCIYTFRGASVRGILDFPKIFSNTKTIFLEKNYRSTTAILNLANAVL
CCCCCEEEEECCCEEEEEECCCCEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
QNFSEKYDKYLFTDNENGSIPNVLQFEDELEEAEGIAEILLQKKEDGIPFKKMCVLFRAG
HHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
WNSNQLELVLAKRNIPFVKFGGRKFIETAHIKDLLSFLKVLVNPLDSVSWIRVLKLIPGI
CCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GNSKANGILDKIRKSSGSFEVLLGENETAIDKYISPLYHLYQKHRETYSEVKKVTSDFID
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYRVLLEKNYDDSKRRSEDLDSVLGFSLKYTSLGDFLSDLTMDHASLSLDKIKPDDAETD
HHHHHHHCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LLNLSTVHSAKGLEFDLVFILNATEGVFPSSKNTDTEEERRLFYVAITRARKELYFTRPS
CEEHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHEEEEEEEHHHHHHHEECCCC
LAHSRSGPYYTKLSRFLSEIQSPEKVYELKLIPGKSVPQNFFSTKVSSSKTANDSFSKIQ
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHH
DYFGN
HHHCC
>Mature Secondary Structure 
SWKEELNAAQLEAVLTQEGPVLVLAGAGTGKTKTIVSRLAQLVSSGVPTSSILLLTFTR
CCHHHCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECH
KAAREMLLRASSIGDKRCTEVQGGTFHSFCSGVLRKFAPVLGLSSGFTILDESDTLDVFQ
HHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHH
FLRNEKDFGKTKSRFPSNETLISIYGEIQNTGRVLQTVLEKDYPLFLQRTKDISQIFEDY
HHHCCCCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KSYKTERSLLDYDDLLYFTRDLLTNHPGVRNALSEKYKFIMVDEFQDTNKVQAHIACLLA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHEEE
SEHSNLMVVGDDAQCIYTFRGASVRGILDFPKIFSNTKTIFLEKNYRSTTAILNLANAVL
CCCCCEEEEECCCEEEEEECCCCEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
QNFSEKYDKYLFTDNENGSIPNVLQFEDELEEAEGIAEILLQKKEDGIPFKKMCVLFRAG
HHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
WNSNQLELVLAKRNIPFVKFGGRKFIETAHIKDLLSFLKVLVNPLDSVSWIRVLKLIPGI
CCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GNSKANGILDKIRKSSGSFEVLLGENETAIDKYISPLYHLYQKHRETYSEVKKVTSDFID
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYRVLLEKNYDDSKRRSEDLDSVLGFSLKYTSLGDFLSDLTMDHASLSLDKIKPDDAETD
HHHHHHHCCCCHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LLNLSTVHSAKGLEFDLVFILNATEGVFPSSKNTDTEEERRLFYVAITRARKELYFTRPS
CEEHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHEEEEEEEHHHHHHHEECCCC
LAHSRSGPYYTKLSRFLSEIQSPEKVYELKLIPGKSVPQNFFSTKVSSSKTANDSFSKIQ
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHHHHH
DYFGN
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]