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The map label for this gene is nth [H]

Identifier: 116328130

GI number: 116328130

Start: 1662394

End: 1663092

Strand: Direct

Name: nth [H]

Synonym: LBL_1428

Alternate gene names: 116328130

Gene position: 1662394-1663092 (Clockwise)

Preceding gene: 116328129

Following gene: 116328131

Centisome position: 45.99

GC content: 40.49

Gene sequence:

>699_bases
TTGCTCCGAAAAAATACGCAGGAATCACAAAACAGGCACTTAAAAAAGCCTGACCCTGCGTTTTTAAAGTGGTTTTCCCG
GATTTTTTCTCTTTTAAGAAAAGAATTCGGGGATGTACAAACCCCTCTTCATTTTAAACATAATTACGAACTTGCTATTG
CCGTCATTTTGAGTGCTCAATGCACGGACGAACGAGTCAATCAAGTTACTCCCTCTCTTTTTAAAACCTTTCCCAGTCTG
GAATCCTTTGCCAATGCGGATCTAAAAGATATCGAAGCTTTGATCTTTTCCACCGGATTTTATCATAATAAAGCGAAATC
AATTCAGGGATTTGCTAAAAAATTGTTAAACGACTTTGACGGGAAAATTCCGAGGACGATCGCGGAGCTTACCACACTTC
CTGGATTTGGTCGTAAGACCGCAAACGTGGTTTTGTCCGAAGTTCACGGACTTGTGGAAGGAATTGTAGTCGATACGCAC
GTAAATCGAATTTCGAAGGTTTTGGGGCTTACCACTAAAAACGATCCGGTTCAGGTAGAGAAAGATCTTATGTCGATTCT
TCCCAAAAAATATTGGAGAGATATTTCCTTGTATTTGATTTTTTTAGGGAGAAAAAGTTGTAAGGCGCATCGCCGATTTT
GTGGAGAATGTATCCTTAAAAAGGACTGCCCATCATCCTCGGTTGTCCAAGGAGTTTGA

Upstream 100 bases:

>100_bases
CGCTGGGTTACGATTCGTCTTTCTACCAGAGCTCTTCGAACTCTGAGAAAAAAAGGAATTAAGGCCGCCATAAAAGATAA
CGGCGGGTCCTTGGGTGTTC

Downstream 100 bases:

>100_bases
TTTTTATGGAAGTATTTATCGATCAAAAATTGGAAGGAATGGAACTTGCTACTCAACATATCGTGATGTCCAGGGATCTC
AATCAACACGGATTTCTTTT

Product: endonuclease III-like protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGDVQTPLHFKHNYELAIAVILSAQCTDERVNQVTPSLFKTFPSL
ESFANADLKDIEALIFSTGFYHNKAKSIQGFAKKLLNDFDGKIPRTIAELTTLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRISKVLGLTTKNDPVQVEKDLMSILPKKYWRDISLYLIFLGRKSCKAHRRFCGECILKKDCPSSSVVQGV

Sequences:

>Translated_232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGDVQTPLHFKHNYELAIAVILSAQCTDERVNQVTPSLFKTFPSL
ESFANADLKDIEALIFSTGFYHNKAKSIQGFAKKLLNDFDGKIPRTIAELTTLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRISKVLGLTTKNDPVQVEKDLMSILPKKYWRDISLYLIFLGRKSCKAHRRFCGECILKKDCPSSSVVQGV
>Mature_232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGDVQTPLHFKHNYELAIAVILSAQCTDERVNQVTPSLFKTFPSL
ESFANADLKDIEALIFSTGFYHNKAKSIQGFAKKLLNDFDGKIPRTIAELTTLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRISKVLGLTTKNDPVQVEKDLMSILPKKYWRDISLYLIFLGRKSCKAHRRFCGECILKKDCPSSSVVQGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=30.5555555555556, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1787920, Length=196, Percent_Identity=35.2040816326531, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=36.2637362637363, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324530, Length=197, Percent_Identity=30.4568527918782, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550361, Length=185, Percent_Identity=30.8108108108108, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26294; Mature: 26294

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGDVQTPLHFKHNYELAIAVILSAQ
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHH
CTDERVNQVTPSLFKTFPSLESFANADLKDIEALIFSTGFYHNKAKSIQGFAKKLLNDFD
CHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GKIPRTIAELTTLPGFGRKTANVVLSEVHGLVEGIVVDTHVNRISKVLGLTTKNDPVQVE
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDLMSILPKKYWRDISLYLIFLGRKSCKAHRRFCGECILKKDCPSSSVVQGV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGDVQTPLHFKHNYELAIAVILSAQ
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHH
CTDERVNQVTPSLFKTFPSLESFANADLKDIEALIFSTGFYHNKAKSIQGFAKKLLNDFD
CHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GKIPRTIAELTTLPGFGRKTANVVLSEVHGLVEGIVVDTHVNRISKVLGLTTKNDPVQVE
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDLMSILPKKYWRDISLYLIFLGRKSCKAHRRFCGECILKKDCPSSSVVQGV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]