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The map label for this gene is trpF

Identifier: 116328120

GI number: 116328120

Start: 1652864

End: 1653514

Strand: Reverse

Name: trpF

Synonym: LBL_1418

Alternate gene names: 116328120

Gene position: 1653514-1652864 (Counterclockwise)

Preceding gene: 116328122

Following gene: 116328119

Centisome position: 45.75

GC content: 42.7

Gene sequence:

>651_bases
ATGAACGAGAATACTTTCGAAAAGACAAAGATTAAAATCTGCGGAATTCGAGACCTCGAAATCGCAAAAATCTGTCGGGA
AGAAGGAGCCGATTATATCGGACTGAATTTTGTTCCTTCCAGCCCCAGAAAGATCTCGCTCAAAGATGCTCAAAAGATAG
TGGAATTTTACAGAGACACAAAAAATTCCCCCGAAATCGTATTGTTGTTTTATCAAAATTCGCCCGAAGAAATCCGAACG
GTCACTTCCTCTTTATACCACGATCTTATTCAATGGGTTTGGGACGATCCGAAACTCACTTTCGTAGATCGGAAAAATTT
TCTCGGAAAACGCCAGATTTGCTCTTATAGAGTAAACTCCCCCATTTACAACGAGGATCTCAAAAACATCCCCAGTGAAC
TTCTAATCTTGGATAGTTATTCCAAAGACGCCGGCGGGGGAACCGGAGAAACTTTCAACTGGAACTTCATTTCACGGATT
GAAAGAAAGTTTCTTTTGGCAGGGGGGCTTAATTCGTCTAACGTCTCGAATGCGATTCGAACCGTAAAGCCGTACGGCGT
CGACGTTGCAAGCGGAGTCGAGTCCTCTCCCGGAATCAAAGATTCACAAAAAGTAATTCAATTTATCAGGAACGTAAGGT
TGGGCTTATGA

Upstream 100 bases:

>100_bases
AATGAAATAAGGCCATCAAATCCGAAAGTATCTTTCCTGCAGAAACTGACCAGTGTTAAAGTACGTGATCTCAAGAAACC
CTTCGGGAAGACTGGAAACC

Downstream 100 bases:

>100_bases
ATTTAGAAATTACAGGAACATCCGCAGAGTACTGGAGCGATCTGGCGGAAGCGCTTAATACTCCAATGATGAAACAGTTT
CTAACGATCAAAAAGGATTT

Product: phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNENTFEKTKIKICGIRDLEIAKICREEGADYIGLNFVPSSPRKISLKDAQKIVEFYRDTKNSPEIVLLFYQNSPEEIRT
VTSSLYHDLIQWVWDDPKLTFVDRKNFLGKRQICSYRVNSPIYNEDLKNIPSELLILDSYSKDAGGGTGETFNWNFISRI
ERKFLLAGGLNSSNVSNAIRTVKPYGVDVASGVESSPGIKDSQKVIQFIRNVRLGL

Sequences:

>Translated_216_residues
MNENTFEKTKIKICGIRDLEIAKICREEGADYIGLNFVPSSPRKISLKDAQKIVEFYRDTKNSPEIVLLFYQNSPEEIRT
VTSSLYHDLIQWVWDDPKLTFVDRKNFLGKRQICSYRVNSPIYNEDLKNIPSELLILDSYSKDAGGGTGETFNWNFISRI
ERKFLLAGGLNSSNVSNAIRTVKPYGVDVASGVESSPGIKDSQKVIQFIRNVRLGL
>Mature_216_residues
MNENTFEKTKIKICGIRDLEIAKICREEGADYIGLNFVPSSPRKISLKDAQKIVEFYRDTKNSPEIVLLFYQNSPEEIRT
VTSSLYHDLIQWVWDDPKLTFVDRKNFLGKRQICSYRVNSPIYNEDLKNIPSELLILDSYSKDAGGGTGETFNWNFISRI
ERKFLLAGGLNSSNVSNAIRTVKPYGVDVASGVESSPGIKDSQKVIQFIRNVRLGL

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Saccharomyces cerevisiae, GI6320210, Length=219, Percent_Identity=31.5068493150685, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_LEPBJ (Q04RT3)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801145.1
- ProteinModelPortal:   Q04RT3
- SMR:   Q04RT3
- STRING:   Q04RT3
- GeneID:   4410106
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1866
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   ETTGITS
- PhylomeDB:   Q04RT3
- ProtClustDB:   CLSK574243
- BioCyc:   LBOR355277:LBJ_1866-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24548; Mature: 24548

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENTFEKTKIKICGIRDLEIAKICREEGADYIGLNFVPSSPRKISLKDAQKIVEFYRDT
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCEECHHHHHHHHHHHHCC
KNSPEIVLLFYQNSPEEIRTVTSSLYHDLIQWVWDDPKLTFVDRKNFLGKRQICSYRVNS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHEECCC
PIYNEDLKNIPSELLILDSYSKDAGGGTGETFNWNFISRIERKFLLAGGLNSSNVSNAIR
CCCCHHHHCCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
TVKPYGVDVASGVESSPGIKDSQKVIQFIRNVRLGL
HCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNENTFEKTKIKICGIRDLEIAKICREEGADYIGLNFVPSSPRKISLKDAQKIVEFYRDT
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCEECHHHHHHHHHHHHCC
KNSPEIVLLFYQNSPEEIRTVTSSLYHDLIQWVWDDPKLTFVDRKNFLGKRQICSYRVNS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHEECCC
PIYNEDLKNIPSELLILDSYSKDAGGGTGETFNWNFISRIERKFLLAGGLNSSNVSNAIR
CCCCHHHHCCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
TVKPYGVDVASGVESSPGIKDSQKVIQFIRNVRLGL
HCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA