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The map label for this gene is sppA-2 [H]

Identifier: 116328102

GI number: 116328102

Start: 1630820

End: 1631812

Strand: Reverse

Name: sppA-2 [H]

Synonym: LBL_1399

Alternate gene names: 116328102

Gene position: 1631812-1630820 (Counterclockwise)

Preceding gene: 116328107

Following gene: 116328101

Centisome position: 45.15

GC content: 43.1

Gene sequence:

>993_bases
TTGAAACACTCGATTCTAACTTGGGCCCATATTCTTTTTTTAAGTTTTTTTCTTTCCAATTGTTATATTCCGGTAAATGC
AAATTTAGGCGCCCCCTCCTCGAAAACCGCAGAATTGAGGGAAAAACTTCTCTCCGGTAAAAACGAAGATAAAATTCTCA
TTATTCCCATAGACGGGGTAATCAGCGACGAAGGAGAAAAAACTTTTTTCGGGGGCCAAGAAGATTCGATCTTAGCCGGA
GTCAAAAAACAACTCGAACTCGCCGAACTGGATCCTGAGATCAAAGCCGTGATTTTGAAGATCAATTCACCAGGAGGTTC
TGTAACCGCAAGCGACATCCTTTACAAAGAGATTCTTCAATTTAAAGCGAAAAAGAAAATTCCCGTGGTCTCCTTGTTTA
TGGATACTGCGGCGAGCGGTGCGTATTATATTTCGATGGCGACCGATCTCATCATCGCACATCCTACTTCCGTAACCGGT
TCCATCGGAGTGATTCTTTCCGGAATCAATGTAAAAGAGGGTTTGGACAAACTCGGAGTTAAGGATCAGTCGATCCGTTC
TGGTGGAAATAAAACCATCGGCTCTCCCTTAGAAGATCTATCTCCCGAACAAAGAAAACTACTTCAGTCCATCGTGGACG
ATCTTTTTGAAAAATTTTTCGAAGTCGTAAAGAACGGAAGACCCGGAAAAAATCCCGCTGAACTTCGCAAAATTGCCGAC
GGAAGAATTTTCACGGCGTCCCAAGCCCTCGGTCACGGGCTAATTGATAAGATCGGTTATTTTGACAACGCGGTTCAAGA
GACGATGGCCCTCCCGAACTACAGAAAAACTCCGGGAAGCAATAGCCCGAGAATCATCTACTATTCTCAGAGTACGGACA
AAAGGGCGAACTTTTATCAGGTTCAATCTCCCGAGTTAAGACAGGATTCTACAATCTCGAAAATTCTCGGAATAAGAAGT
CAGGTTCGTTTTCTATATATCTGGTCTTATTAA

Upstream 100 bases:

>100_bases
CTAAACCTAACCACGAATCACTCGAAGCATTCCCTTAAAATAGATGGAAAGGAATTGAAAGCTTGTTTAACTAGGGAAAA
TTGACGATAGGAGAATTTCT

Downstream 100 bases:

>100_bases
GTTCCATTAGAAATTTAGAATCTATTTTCCGGTATATCAATGTTATTCAATTCTTTACCCTTTCTTTTCTTATTTCTAAT
CACGTATTTGATCTACTGGA

Product: periplasmic serine protease (ClpP class)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKHSILTWAHILFLSFFLSNCYIPVNANLGAPSSKTAELREKLLSGKNEDKILIIPIDGVISDEGEKTFFGGQEDSILAG
VKKQLELAELDPEIKAVILKINSPGGSVTASDILYKEILQFKAKKKIPVVSLFMDTAASGAYYISMATDLIIAHPTSVTG
SIGVILSGINVKEGLDKLGVKDQSIRSGGNKTIGSPLEDLSPEQRKLLQSIVDDLFEKFFEVVKNGRPGKNPAELRKIAD
GRIFTASQALGHGLIDKIGYFDNAVQETMALPNYRKTPGSNSPRIIYYSQSTDKRANFYQVQSPELRQDSTISKILGIRS
QVRFLYIWSY

Sequences:

>Translated_330_residues
MKHSILTWAHILFLSFFLSNCYIPVNANLGAPSSKTAELREKLLSGKNEDKILIIPIDGVISDEGEKTFFGGQEDSILAG
VKKQLELAELDPEIKAVILKINSPGGSVTASDILYKEILQFKAKKKIPVVSLFMDTAASGAYYISMATDLIIAHPTSVTG
SIGVILSGINVKEGLDKLGVKDQSIRSGGNKTIGSPLEDLSPEQRKLLQSIVDDLFEKFFEVVKNGRPGKNPAELRKIAD
GRIFTASQALGHGLIDKIGYFDNAVQETMALPNYRKTPGSNSPRIIYYSQSTDKRANFYQVQSPELRQDSTISKILGIRS
QVRFLYIWSY
>Mature_330_residues
MKHSILTWAHILFLSFFLSNCYIPVNANLGAPSSKTAELREKLLSGKNEDKILIIPIDGVISDEGEKTFFGGQEDSILAG
VKKQLELAELDPEIKAVILKINSPGGSVTASDILYKEILQFKAKKKIPVVSLFMDTAASGAYYISMATDLIIAHPTSVTG
SIGVILSGINVKEGLDKLGVKDQSIRSGGNKTIGSPLEDLSPEQRKLLQSIVDDLFEKFFEVVKNGRPGKNPAELRKIAD
GRIFTASQALGHGLIDKIGYFDNAVQETMALPNYRKTPGSNSPRIIYYSQSTDKRANFYQVQSPELRQDSTISKILGIRS
QVRFLYIWSY

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=233, Percent_Identity=34.7639484978541, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787527, Length=183, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 36332; Mature: 36332

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSILTWAHILFLSFFLSNCYIPVNANLGAPSSKTAELREKLLSGKNEDKILIIPIDGV
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCE
ISDEGEKTFFGGQEDSILAGVKKQLELAELDPEIKAVILKINSPGGSVTASDILYKEILQ
ECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
FKAKKKIPVVSLFMDTAASGAYYISMATDLIIAHPTSVTGSIGVILSGINVKEGLDKLGV
HHHHCCCCEEEEEHHHCCCCEEEEEEECEEEEECCCCCCCCEEEEEECCCHHHHHHHHCC
KDQSIRSGGNKTIGSPLEDLSPEQRKLLQSIVDDLFEKFFEVVKNGRPGKNPAELRKIAD
CCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GRIFTASQALGHGLIDKIGYFDNAVQETMALPNYRKTPGSNSPRIIYYSQSTDKRANFYQ
CCEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
VQSPELRQDSTISKILGIRSQVRFLYIWSY
ECCCCCCCHHHHHHHHHHHHCEEEEEEECC
>Mature Secondary Structure
MKHSILTWAHILFLSFFLSNCYIPVNANLGAPSSKTAELREKLLSGKNEDKILIIPIDGV
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCE
ISDEGEKTFFGGQEDSILAGVKKQLELAELDPEIKAVILKINSPGGSVTASDILYKEILQ
ECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
FKAKKKIPVVSLFMDTAASGAYYISMATDLIIAHPTSVTGSIGVILSGINVKEGLDKLGV
HHHHCCCCEEEEEHHHCCCCEEEEEEECEEEEECCCCCCCCEEEEEECCCHHHHHHHHCC
KDQSIRSGGNKTIGSPLEDLSPEQRKLLQSIVDDLFEKFFEVVKNGRPGKNPAELRKIAD
CCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GRIFTASQALGHGLIDKIGYFDNAVQETMALPNYRKTPGSNSPRIIYYSQSTDKRANFYQ
CCEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
VQSPELRQDSTISKILGIRSQVRFLYIWSY
ECCCCCCCHHHHHHHHHHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]