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The map label for this gene is cysK-3 [H]

Identifier: 116328076

GI number: 116328076

Start: 1604830

End: 1605831

Strand: Direct

Name: cysK-3 [H]

Synonym: LBL_1373

Alternate gene names: 116328076

Gene position: 1604830-1605831 (Clockwise)

Preceding gene: 116328075

Following gene: 116328077

Centisome position: 44.4

GC content: 43.41

Gene sequence:

>1002_bases
ATGTTTGATGAAATATCCCGTTCGATTGACGAATTTGGAAATAGTTTTCTTGGCGCTCTGAACAACATTCAGAAATCTTT
TGGGCGGGAACTTAGCGTAGCAAAACCCGTTAAAGAGACGATCCTACAGATGATCGGAAATACTCCGCTCATTCGCCTGA
ATCAAATCGGCTCTCATATTCCCAACGTAGAATTTTATCTCAAAGCGGAATTTTGTAATCCTACCGGTTCTGTAAAAGAT
AGGACCGCATTATCCATGCTTCTTTCTTCCGAAAGAAGAGGAGAATTAAAACCCAAGGGACAGGTTATTCAACCCGGTTA
CAACACGACCGCGATCAGTCTTGCTTGGATTTGTACAATTCGACAATATAAATTTCGTTGTTTGGTTGCCGGTGATACCG
ATCCGCAAAAGATCAAAGACCTGCAAACCTTCGGAGCTCAAGTAGAGGTCGTTCCCGAAGCGAAAGGAAATTGGGACGAA
ACCTTCTTAAGGAAAGCGAAGGAAATCAAAGAAAAAGAAAAAAACGTAGTGATTCTAAACGAATACAAAGATATGGCGAA
CACGAATGCACATTATCTTTTTACTGGACCCGAAATCTGGAGAGATCTCGGCGGAAGTGTGGATGCGTTTGTAGCCGGAG
GAGGCTCTGGAGGAACTCTTTCCGGTGTGGGTAGATTTTTAAAATCCAAAAAATCTTCGGTTCGAATCATCATGGGAGTC
AGTCAAAAATCCAGATTCATCCGTAAGATGGTTCAAAAGGAAAGTTCCATTCATCTTCCTGAATCCTTTGATCCGAAGAT
CGTAGACGAATACGTCGGAGTCGATCGGGAACAGGCGCTTTTATATCAATCCGATCTTTATCAAAAAGAGGGGATTTTTG
CGGGACAGACAACCGGAACTACTCTTGCGAGCGCGATTCGATTTGCGGAAGGACTCCCCATAAGAGGCGATCAAAAATCC
CAGGTCTACAGAATCGTAGTCCTTTCTCCCGATCGGTTTTGA

Upstream 100 bases:

>100_bases
CGGTCTTACTCTCAAGCAGGGAAGTAAAATCGGAGCATTTGAAAAAAAACAGAAAGAAGTTGAACCTTGGTTGTACGTTA
TATAATTCTGCCAAAAATTT

Downstream 100 bases:

>100_bases
AAGAAAGAAAATTTTCCAGCTCGATCGAGGAGTTGGAATTTGTTCGGGCTGAACTCGAAAGGGAAAAAAAAGAAGAGGGT
TTCCTCTTTTCCAAAGACTG

Product: cysteine synthase

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFDEISRSIDEFGNSFLGALNNIQKSFGRELSVAKPVKETILQMIGNTPLIRLNQIGSHIPNVEFYLKAEFCNPTGSVKD
RTALSMLLSSERRGELKPKGQVIQPGYNTTAISLAWICTIRQYKFRCLVAGDTDPQKIKDLQTFGAQVEVVPEAKGNWDE
TFLRKAKEIKEKEKNVVILNEYKDMANTNAHYLFTGPEIWRDLGGSVDAFVAGGGSGGTLSGVGRFLKSKKSSVRIIMGV
SQKSRFIRKMVQKESSIHLPESFDPKIVDEYVGVDREQALLYQSDLYQKEGIFAGQTTGTTLASAIRFAEGLPIRGDQKS
QVYRIVVLSPDRF

Sequences:

>Translated_333_residues
MFDEISRSIDEFGNSFLGALNNIQKSFGRELSVAKPVKETILQMIGNTPLIRLNQIGSHIPNVEFYLKAEFCNPTGSVKD
RTALSMLLSSERRGELKPKGQVIQPGYNTTAISLAWICTIRQYKFRCLVAGDTDPQKIKDLQTFGAQVEVVPEAKGNWDE
TFLRKAKEIKEKEKNVVILNEYKDMANTNAHYLFTGPEIWRDLGGSVDAFVAGGGSGGTLSGVGRFLKSKKSSVRIIMGV
SQKSRFIRKMVQKESSIHLPESFDPKIVDEYVGVDREQALLYQSDLYQKEGIFAGQTTGTTLASAIRFAEGLPIRGDQKS
QVYRIVVLSPDRF
>Mature_333_residues
MFDEISRSIDEFGNSFLGALNNIQKSFGRELSVAKPVKETILQMIGNTPLIRLNQIGSHIPNVEFYLKAEFCNPTGSVKD
RTALSMLLSSERRGELKPKGQVIQPGYNTTAISLAWICTIRQYKFRCLVAGDTDPQKIKDLQTFGAQVEVVPEAKGNWDE
TFLRKAKEIKEKEKNVVILNEYKDMANTNAHYLFTGPEIWRDLGGSVDAFVAGGGSGGTLSGVGRFLKSKKSSVRIIMGV
SQKSRFIRKMVQKESSIHLPESFDPKIVDEYVGVDREQALLYQSDLYQKEGIFAGQTTGTTLASAIRFAEGLPIRGDQKS
QVYRIVVLSPDRF

Specific function: Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=32.7868852459016, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=32.7868852459016, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=32.7868852459016, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI2367138, Length=297, Percent_Identity=31.6498316498317, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788754, Length=301, Percent_Identity=32.890365448505, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17535051, Length=291, Percent_Identity=32.9896907216495, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI32566674, Length=272, Percent_Identity=30.1470588235294, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562970, Length=272, Percent_Identity=30.1470588235294, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115535073, Length=285, Percent_Identity=30.8771929824561, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17561720, Length=272, Percent_Identity=29.7794117647059, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=29.0220820189274, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566672, Length=175, Percent_Identity=28, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17539800, Length=219, Percent_Identity=23.7442922374429, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=28.9808917197452, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6321449, Length=315, Percent_Identity=31.1111111111111, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=30.8441558441558, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=30.8441558441558, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005858
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 37114; Mature: 37114

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDEISRSIDEFGNSFLGALNNIQKSFGRELSVAKPVKETILQMIGNTPLIRLNQIGSHI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCC
PNVEFYLKAEFCNPTGSVKDRTALSMLLSSERRGELKPKGQVIQPGYNTTAISLAWICTI
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHH
RQYKFRCLVAGDTDPQKIKDLQTFGAQVEVVPEAKGNWDETFLRKAKEIKEKEKNVVILN
HHEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
EYKDMANTNAHYLFTGPEIWRDLGGSVDAFVAGGGSGGTLSGVGRFLKSKKSSVRIIMGV
CCHHHCCCCCEEEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEC
SQKSRFIRKMVQKESSIHLPESFDPKIVDEYVGVDREQALLYQSDLYQKEGIFAGQTTGT
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHH
TLASAIRFAEGLPIRGDQKSQVYRIVVLSPDRF
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MFDEISRSIDEFGNSFLGALNNIQKSFGRELSVAKPVKETILQMIGNTPLIRLNQIGSHI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCC
PNVEFYLKAEFCNPTGSVKDRTALSMLLSSERRGELKPKGQVIQPGYNTTAISLAWICTI
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHH
RQYKFRCLVAGDTDPQKIKDLQTFGAQVEVVPEAKGNWDETFLRKAKEIKEKEKNVVILN
HHEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
EYKDMANTNAHYLFTGPEIWRDLGGSVDAFVAGGGSGGTLSGVGRFLKSKKSSVRIIMGV
CCHHHCCCCCEEEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEC
SQKSRFIRKMVQKESSIHLPESFDPKIVDEYVGVDREQALLYQSDLYQKEGIFAGQTTGT
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHH
TLASAIRFAEGLPIRGDQKSQVYRIVVLSPDRF
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503 [H]