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The map label for this gene is fliM [H]

Identifier: 116328061

GI number: 116328061

Start: 1588936

End: 1589961

Strand: Direct

Name: fliM [H]

Synonym: LBL_1356

Alternate gene names: 116328061

Gene position: 1588936-1589961 (Clockwise)

Preceding gene: 116328060

Following gene: 116328063

Centisome position: 43.96

GC content: 44.44

Gene sequence:

>1026_bases
ATGACGGAGATTCTATCGCAAGATGAAATTGACGCGCTACTAAGCGCTATCAGTTCCGGTGAAGTAAGCGAATCGGATTA
TACTTCCGTTTCCGAGCAAAAGAAAGTAAAGATCTACGACTTCAAACGCCCTGATAAATTTTCAAAGGACCAAATTCGTA
CTTTGCAAATGATGCACGAAACCTTTGCTCGTTTGGCGACTACGGGTCTTTCGGCACAGCTTAGAGCGCTCGTATCCGTT
CACGTCGCTTCCGTGGATCAGTTGACTTATGAAGAATTCATTCGTTCGATTCCGAATCCGACCACTCTTGCCGTAATTAA
TATGGATCCGCTTCGCGGTTCCGCGATTTTAGAGATTGATCCTTCGATTTCGTTTACGATCATCGATCGTCTGTTTGGCG
GTAAGGGAGAGCAGGCAAAAATCTCCAGAGAACTTTCCGAGATAGAGATGAGTGTAATGGAAGGGATTATCGTTCGAATT
CTCGGGAACATGAGGGAATCTTGGTCTACCGTAATCGATTTAAGACCGAGGCTTGGGAATATCGAAACGAATCCTCAATT
TGCGCAGGTCGTTCCACCGAACGACATGGTGGTTTTGATCACTCTCGAAACCAAAATCGGAGAAGTGGAAGGGATGACAA
ACCTTTGTATTCCCTATATCACCATCGAACCGATCATCAATAAATTATCCGCACAATATTGGTATTCGTCTATTCGAAAA
GGGGAATTGGATGAGAACCGCGCCGTAATTCAAGAGCGACTGGATCAGGTTGCAATTCCTTTGATCGCGGAAGTGGGATC
TGTGGACGTTTCCATCAATGATTTTATGAATCTTTCCGTGGGTGATGTTGTGAAACTGGAAAACACTTCCACGAGATCCG
AGATGATCGTAAAAGTTGGGGAAAGAAAAAAATTCAAATGTCTTCCTGGAAGAGTGGGGAGCAGGCTTGCGATTCAAATT
GGAGATAGGGTCGAAGATATTCCGGATGAACTTCTGGGTTCCACACGTTCTGAACAAGAATATTAG

Upstream 100 bases:

>100_bases
GCCTGCGCTCCAAATACGAATATATTTATACTCTCAGAAGGGTTCCCGACGAACAGGGAGGTTTCTGGAAAGTGTCTAAC
TTAGTGGCGAAGGTAAAGAA

Downstream 100 bases:

>100_bases
AGTTTGAATTTAGGCTCTTCTTAAAACCTGAAGAAAAATATCCCTAAACGATCCGGCAAGTTTCTAAAAAAACGTGTGAG
TTCCTACATTCAGAGAACTG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTEILSQDEIDALLSAISSGEVSESDYTSVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRI
LGNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSVGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQI
GDRVEDIPDELLGSTRSEQEY

Sequences:

>Translated_341_residues
MTEILSQDEIDALLSAISSGEVSESDYTSVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRI
LGNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSVGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQI
GDRVEDIPDELLGSTRSEQEY
>Mature_340_residues
TEILSQDEIDALLSAISSGEVSESDYTSVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHETFARLATTGLSAQLRALVSVH
VASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRIL
GNMRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRKG
ELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSVGDVVKLENTSTRSEMIVKVGERKKFKCLPGRVGSRLAIQIG
DRVEDIPDELLGSTRSEQEY

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=339, Percent_Identity=28.023598820059, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 38112; Mature: 37981

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEILSQDEIDALLSAISSGEVSESDYTSVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TFARLATTGLSAQLRALVSVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRILGNMRESWSTVIDLRPRLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCC
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSVGDVVKLENTSTRSEMIVKVG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCHHHHHHHHC
ERKKFKCLPGRVGSRLAIQIGDRVEDIPDELLGSTRSEQEY
CCCCEEECCCCCCCEEEEEECCHHHHCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TEILSQDEIDALLSAISSGEVSESDYTSVSEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
TFARLATTGLSAQLRALVSVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGEQAKISRELSEIEMSVMEGIIVRILGNMRESWSTVIDLRPRLGN
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKIGEVEGMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCC
GELDENRAVIQERLDQVAIPLIAEVGSVDVSINDFMNLSVGDVVKLENTSTRSEMIVKVG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEEECCCCCHHHHHHHHC
ERKKFKCLPGRVGSRLAIQIGDRVEDIPDELLGSTRSEQEY
CCCCEEECCCCCCCEEEEEECCHHHHCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]