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The map label for this gene is dam [H]

Identifier: 116328052

GI number: 116328052

Start: 1580631

End: 1581494

Strand: Reverse

Name: dam [H]

Synonym: LBL_1347

Alternate gene names: 116328052

Gene position: 1581494-1580631 (Counterclockwise)

Preceding gene: 116328053

Following gene: 116328050

Centisome position: 43.75

GC content: 37.04

Gene sequence:

>864_bases
ATGGAATCGCAATTGGTTAAAAGTCCGCTAAAAGGACAACTTTTAAAATGGATTGGAAACAAGCAAAAATATGCGGATAC
GATCATCTCTTATTTTCCTAAAAAAATAAAAGCCTACCGCGAACCTTTTTTAGGCAGCGGTGCAGTTATAGCAACCTTAT
CTCCTCAAAAAGGTATTGGATCCGATATATTCAAACCGCTGATTGATATTTTTCAGAAGTTGAAATCAAACCCTGATGAG
TTGATTCTAGACTATACCGAAAATTATGCACTGATAGAAAAATTCGGAAAAGAAAAAGCTTATGAAATCATAAAGAGTTC
GTATAATCTCAACCCGAATCCTAAAGACTTATTGTTTTTATCCCGTTCCTGTTATGGCGGCGTAGTAAGATTCCGTAAAG
TAGACGCTTACATGTCAACTCCTTGTGGAATTCACAATCCACTTTCTCCGAAATCCTTTGCTGAAAGAGTTTATTCCTGG
AGTAATCGCGTCGCAGATTGTGAATTTTTAAACTTAAATTACAAAGACGCTTTCGATGCCGCAGAAGCGGATGATTTGAT
ATATTGTGATCCTCCCTATAAGGATTCACAGCCTATACTGTACGGGGCTCAATCCTTTTCTCTTAAAGATTTATATGACA
GTATTGAAAACGCAAAATTACGAGGGGTAAAAATCGCCCTTAGCATCGATGGCAGTAAAAAATCAGGTTCCAAAATTATC
GATATTCAATATCCGAAAGGACTTTTCTCTTCGGAAATTCCTATATTAAACGGACGTTCCATGCTGAAGCGTTTTCAAAT
GGCGGGACAAACTTTAGAGGAGGAAGTTGTTACGGAAAGATTGTTGCTCACTTATAAAATTTAG

Upstream 100 bases:

>100_bases
TATGAAAAAGGACTCTGCTTCATAGGTAAAATGGATTCCACGTTTTTCCAGTTGCCCTCGTTTAAGGTATTTTGATTATA
TAATCATGTTTCTTTTCCCA

Downstream 100 bases:

>100_bases
AAATTATCTTTACAAATGTCGAAAACAACTTGAAACGAAAATATGCGATTTCGAATTTAAAGTCTTCTGCTTTGATCCAC
AATTTCCGTAATTTGAATTC

Product: site-specific DNA-methyltransferase (adenine- specific), dam

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.SmaII [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MESQLVKSPLKGQLLKWIGNKQKYADTIISYFPKKIKAYREPFLGSGAVIATLSPQKGIGSDIFKPLIDIFQKLKSNPDE
LILDYTENYALIEKFGKEKAYEIIKSSYNLNPNPKDLLFLSRSCYGGVVRFRKVDAYMSTPCGIHNPLSPKSFAERVYSW
SNRVADCEFLNLNYKDAFDAAEADDLIYCDPPYKDSQPILYGAQSFSLKDLYDSIENAKLRGVKIALSIDGSKKSGSKII
DIQYPKGLFSSEIPILNGRSMLKRFQMAGQTLEEEVVTERLLLTYKI

Sequences:

>Translated_287_residues
MESQLVKSPLKGQLLKWIGNKQKYADTIISYFPKKIKAYREPFLGSGAVIATLSPQKGIGSDIFKPLIDIFQKLKSNPDE
LILDYTENYALIEKFGKEKAYEIIKSSYNLNPNPKDLLFLSRSCYGGVVRFRKVDAYMSTPCGIHNPLSPKSFAERVYSW
SNRVADCEFLNLNYKDAFDAAEADDLIYCDPPYKDSQPILYGAQSFSLKDLYDSIENAKLRGVKIALSIDGSKKSGSKII
DIQYPKGLFSSEIPILNGRSMLKRFQMAGQTLEEEVVTERLLLTYKI
>Mature_287_residues
MESQLVKSPLKGQLLKWIGNKQKYADTIISYFPKKIKAYREPFLGSGAVIATLSPQKGIGSDIFKPLIDIFQKLKSNPDE
LILDYTENYALIEKFGKEKAYEIIKSSYNLNPNPKDLLFLSRSCYGGVVRFRKVDAYMSTPCGIHNPLSPKSFAERVYSW
SNRVADCEFLNLNYKDAFDAAEADDLIYCDPPYKDSQPILYGAQSFSLKDLYDSIENAKLRGVKIALSIDGSKKSGSKII
DIQYPKGLFSSEIPILNGRSMLKRFQMAGQTLEEEVVTERLLLTYKI

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=205, Percent_Identity=25.3658536585366, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32467; Mature: 32467

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQLVKSPLKGQLLKWIGNKQKYADTIISYFPKKIKAYREPFLGSGAVIATLSPQKGIG
CCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
SDIFKPLIDIFQKLKSNPDELILDYTENYALIEKFGKEKAYEIIKSSYNLNPNPKDLLFL
HHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHEEE
SRSCYGGVVRFRKVDAYMSTPCGIHNPLSPKSFAERVYSWSNRVADCEFLNLNYKDAFDA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCC
AEADDLIYCDPPYKDSQPILYGAQSFSLKDLYDSIENAKLRGVKIALSIDGSKKSGSKII
CCCCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHHCCEECEEEEEEEECCCCCCCCEEE
DIQYPKGLFSSEIPILNGRSMLKRFQMAGQTLEEEVVTERLLLTYKI
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MESQLVKSPLKGQLLKWIGNKQKYADTIISYFPKKIKAYREPFLGSGAVIATLSPQKGIG
CCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
SDIFKPLIDIFQKLKSNPDELILDYTENYALIEKFGKEKAYEIIKSSYNLNPNPKDLLFL
HHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCHHHEEE
SRSCYGGVVRFRKVDAYMSTPCGIHNPLSPKSFAERVYSWSNRVADCEFLNLNYKDAFDA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCC
AEADDLIYCDPPYKDSQPILYGAQSFSLKDLYDSIENAKLRGVKIALSIDGSKKSGSKII
CCCCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHHCCEECEEEEEEEECCCCCCCCEEE
DIQYPKGLFSSEIPILNGRSMLKRFQMAGQTLEEEVVTERLLLTYKI
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10383952 [H]