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The map label for this gene is ddlA

Identifier: 116328043

GI number: 116328043

Start: 1571333

End: 1572388

Strand: Direct

Name: ddlA

Synonym: LBL_1338

Alternate gene names: 116328043

Gene position: 1571333-1572388 (Clockwise)

Preceding gene: 116328037

Following gene: 116328046

Centisome position: 43.47

GC content: 45.08

Gene sequence:

>1056_bases
TTGGCTAAGATCGCGGTTTTTTTTGGTGGTAGTTCTACGGAACACAGTATTTCGATTCGAACAGGTTGTTTTATTTGTAG
GACTCTGCATACGATGGGGCATTCGGTCAAACCCATTCTCTTGACTCAGGACGGAGGTTGGGTGGTTCCTTTACAATATC
GGATATCAATTCCATATGAAGCGGTGAATTCTTCGGATTTGTTTGAGGAAGAATTTCAAAAAACAAACGGTGTATCTAAA
ATGGATTTTATTTCCAACTTGGATGCGGATATCGTTTTTCTCGGTCTTCACGGCGGAAAAGGAGAAGACGGAAGTATTCA
AGGTTTCCTAAGAGTTCTGGGGGTTCCTTATACCGGTTCCGGGGTCGCCGCTTCCGCGCTTGCAATGGATAAAACCAGAG
CCAATCAGATTTTTTTACAATCCGGTCAAAAGGTCGCTCCTTTTTTTGAAGTCGAGAAGTTAGGATATACGAATTCTCCC
GAAGAAACTGTCATCAAGTTGATGAGTTTGGGATTTCCTCAGTTTTTAAAGCCGGTGGAAGGTGGCTCGAGTGTTTCCAC
GTATAAAATTACGAACCAAGAACAACTTTCCCGCCAGCTTGCTCTTATATTTGAATCCGATTCCAAAGTAATGAGCCAGT
CCTTTCTGGCTGGAACCGAAGTTTCCTGCGGAGTTTTGGAAAGATATAGAAACGGAAAGTTGGAACGGATCGCGTTACCC
GCGACTGAAATCGTTCCCGGAGGGGAATTTTTCGACTTCGAATCTAAATACAAACAAGGCGGTTCACGCGAGATTACTCC
CGCAAGGATTTCGAAACAGGAGATGACGCGTGTACAGGAACTTGCAATAGACGCACACACGTCTCTCGGGTGCAGGGGAT
ACTCAAGAAGCGATTTTATCATTGTAGGCGGGGAACCTCATATTCTGGAAACCAACACGTTACCCGGAATGACGGAGACC
AGCTTGATTCCGCAACAAGCAAAAGCCGCGGGAATTACGATGGAGGAAGTGTTTGCTGACCTGATAGAGATCGGACTGAA
ACATTCTATCCATTAG

Upstream 100 bases:

>100_bases
AAAATATGTTTCCAGAGTAAGTAATAAGAATTACGATGCAAAAAATTCTAAATTTTTTCCAGAATTTTGAAATGAATCCA
TTCTTTTTGGGGAATTTTTC

Downstream 100 bases:

>100_bases
AAAACTAGGATATAACAAAAAGCGCCAGTTTTGAAAAGAAGTTGGGACGGAATTTTACACGAGATAGATCTCTGAAATAC
GCTTTGTCTTCGATTTTGAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAKIAVFFGGSSTEHSISIRTGCFICRTLHTMGHSVKPILLTQDGGWVVPLQYRISIPYEAVNSSDLFEEEFQKTNGVSK
MDFISNLDADIVFLGLHGGKGEDGSIQGFLRVLGVPYTGSGVAASALAMDKTRANQIFLQSGQKVAPFFEVEKLGYTNSP
EETVIKLMSLGFPQFLKPVEGGSSVSTYKITNQEQLSRQLALIFESDSKVMSQSFLAGTEVSCGVLERYRNGKLERIALP
ATEIVPGGEFFDFESKYKQGGSREITPARISKQEMTRVQELAIDAHTSLGCRGYSRSDFIIVGGEPHILETNTLPGMTET
SLIPQQAKAAGITMEEVFADLIEIGLKHSIH

Sequences:

>Translated_351_residues
MAKIAVFFGGSSTEHSISIRTGCFICRTLHTMGHSVKPILLTQDGGWVVPLQYRISIPYEAVNSSDLFEEEFQKTNGVSK
MDFISNLDADIVFLGLHGGKGEDGSIQGFLRVLGVPYTGSGVAASALAMDKTRANQIFLQSGQKVAPFFEVEKLGYTNSP
EETVIKLMSLGFPQFLKPVEGGSSVSTYKITNQEQLSRQLALIFESDSKVMSQSFLAGTEVSCGVLERYRNGKLERIALP
ATEIVPGGEFFDFESKYKQGGSREITPARISKQEMTRVQELAIDAHTSLGCRGYSRSDFIIVGGEPHILETNTLPGMTET
SLIPQQAKAAGITMEEVFADLIEIGLKHSIH
>Mature_350_residues
AKIAVFFGGSSTEHSISIRTGCFICRTLHTMGHSVKPILLTQDGGWVVPLQYRISIPYEAVNSSDLFEEEFQKTNGVSKM
DFISNLDADIVFLGLHGGKGEDGSIQGFLRVLGVPYTGSGVAASALAMDKTRANQIFLQSGQKVAPFFEVEKLGYTNSPE
ETVIKLMSLGFPQFLKPVEGGSSVSTYKITNQEQLSRQLALIFESDSKVMSQSFLAGTEVSCGVLERYRNGKLERIALPA
TEIVPGGEFFDFESKYKQGGSREITPARISKQEMTRVQELAIDAHTSLGCRGYSRSDFIIVGGEPHILETNTLPGMTETS
LIPQQAKAAGITMEEVFADLIEIGLKHSIH

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=344, Percent_Identity=32.5581395348837, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=31.1797752808989, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_LEPBJ (Q04RK6)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801222.1
- ProteinModelPortal:   Q04RK6
- SMR:   Q04RK6
- STRING:   Q04RK6
- GeneID:   4411465
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1946
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q04RK6
- ProtClustDB:   PRK14572
- BioCyc:   LBOR355277:LBJ_1946-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38303; Mature: 38172

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIAVFFGGSSTEHSISIRTGCFICRTLHTMGHSVKPILLTQDGGWVVPLQYRISIPYE
CCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCHH
AVNSSDLFEEEFQKTNGVSKMDFISNLDADIVFLGLHGGKGEDGSIQGFLRVLGVPYTGS
HCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
GVAASALAMDKTRANQIFLQSGQKVAPFFEVEKLGYTNSPEETVIKLMSLGFPQFLKPVE
CHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCC
GGSSVSTYKITNQEQLSRQLALIFESDSKVMSQSFLAGTEVSCGVLERYRNGKLERIALP
CCCCEEEEEECCHHHHHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECC
ATEIVPGGEFFDFESKYKQGGSREITPARISKQEMTRVQELAIDAHTSLGCRGYSRSDFI
HHHCCCCCCEECCHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IVGGEPHILETNTLPGMTETSLIPQQAKAAGITMEEVFADLIEIGLKHSIH
EECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKIAVFFGGSSTEHSISIRTGCFICRTLHTMGHSVKPILLTQDGGWVVPLQYRISIPYE
CEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEEEECCHH
AVNSSDLFEEEFQKTNGVSKMDFISNLDADIVFLGLHGGKGEDGSIQGFLRVLGVPYTGS
HCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
GVAASALAMDKTRANQIFLQSGQKVAPFFEVEKLGYTNSPEETVIKLMSLGFPQFLKPVE
CHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCC
GGSSVSTYKITNQEQLSRQLALIFESDSKVMSQSFLAGTEVSCGVLERYRNGKLERIALP
CCCCEEEEEECCHHHHHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECC
ATEIVPGGEFFDFESKYKQGGSREITPARISKQEMTRVQELAIDAHTSLGCRGYSRSDFI
HHHCCCCCCEECCHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IVGGEPHILETNTLPGMTETSLIPQQAKAAGITMEEVFADLIEIGLKHSIH
EECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA