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The map label for this gene is lipB

Identifier: 116327997

GI number: 116327997

Start: 1509425

End: 1510084

Strand: Direct

Name: lipB

Synonym: LBL_1287

Alternate gene names: 116327997

Gene position: 1509425-1510084 (Clockwise)

Preceding gene: 116327993

Following gene: 116327998

Centisome position: 41.76

GC content: 40.61

Gene sequence:

>660_bases
GTGAGAATGATCAGATTTCGGAAAAAAATTCCTTACCTCCGTTACTTGGAAATGCAGGAAAAACTGCGAAAGTTCCGGAG
GGAATGTATTCTATTTTTGGAACATGCTCCCATAATCACCGGCGGAATCAATTATAATCCGGAGAATCTTCTTTTGGGAA
AGGAATTTCTAGAATCTCAAGGAATTCAGGTCCACTTCGTCCAAAGAGGTGGGGATTTCACTGCCCACGAACCCGGCCAG
CTCGTCATCTATCCTCATGTGGACTTAAAAAAAAGAAATCTCCCGATCCGTTTTTATCTGGAGAATTTACTTCAGTCCGT
AATCGATTCTGCTCGTACAACCTGGGGTTTGAATCTGATCACCGATTCGGATTCTCCGGGTTTATATCTCGAATCGAATT
CTTCCAAAAAGATCTGTTCTATCGGCGTGAACTTTAAATCTTTTTTTACGAGTCATGGAGTCGCGTTCAATTTAAATAAC
GATTTTACGGCTTTTCGTTGTATCAATCCTTGCGGTAGAAATTGGACGGATATGACCAGTGTGGAAAAACTCGGGCTCGA
CTCCGGTTTTGCTAAAAGGAACCAATTTATTTCTTTTATGAAAGCGAATCTGAATTCTTTTCTAGGACCTATCTCGAAAT
TAACCCATACTGTGATATAA

Upstream 100 bases:

>100_bases
AATAAATCAGTTTGGAAAGGGATAGACGGTCTCTTCCGTTAGGAGATTTCTGAAGGATAAAAGAGATGACTTCTAAGAGT
TTTTCCATATAAAGAATCTT

Downstream 100 bases:

>100_bases
CGATTCTTGAGAGAAAGTAGGATTTTAGAGATCATTTGGAATCCTTCAAAATGTTTCGTTTTCGATGAAAAGTGAGCTGG
AATTCTTTTTTCTCCTCAAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRMIRFRKKIPYLRYLEMQEKLRKFRRECILFLEHAPIITGGINYNPENLLLGKEFLESQGIQVHFVQRGGDFTAHEPGQ
LVIYPHVDLKKRNLPIRFYLENLLQSVIDSARTTWGLNLITDSDSPGLYLESNSSKKICSIGVNFKSFFTSHGVAFNLNN
DFTAFRCINPCGRNWTDMTSVEKLGLDSGFAKRNQFISFMKANLNSFLGPISKLTHTVI

Sequences:

>Translated_219_residues
MRMIRFRKKIPYLRYLEMQEKLRKFRRECILFLEHAPIITGGINYNPENLLLGKEFLESQGIQVHFVQRGGDFTAHEPGQ
LVIYPHVDLKKRNLPIRFYLENLLQSVIDSARTTWGLNLITDSDSPGLYLESNSSKKICSIGVNFKSFFTSHGVAFNLNN
DFTAFRCINPCGRNWTDMTSVEKLGLDSGFAKRNQFISFMKANLNSFLGPISKLTHTVI
>Mature_219_residues
MRMIRFRKKIPYLRYLEMQEKLRKFRRECILFLEHAPIITGGINYNPENLLLGKEFLESQGIQVHFVQRGGDFTAHEPGQ
LVIYPHVDLKKRNLPIRFYLENLLQSVIDSARTTWGLNLITDSDSPGLYLESNSSKKICSIGVNFKSFFTSHGVAFNLNN
DFTAFRCINPCGRNWTDMTSVEKLGLDSGFAKRNQFISFMKANLNSFLGPISKLTHTVI

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=201, Percent_Identity=31.3432835820896, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87081767, Length=154, Percent_Identity=33.7662337662338, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323268, Length=201, Percent_Identity=30.8457711442786, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355483, Length=184, Percent_Identity=28.804347826087, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_LEPBJ (Q04TD5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800593.1
- ProteinModelPortal:   Q04TD5
- SMR:   Q04TD5
- STRING:   Q04TD5
- GeneID:   4411025
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1236
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   TEDHERP
- PhylomeDB:   Q04TD5
- ProtClustDB:   CLSK574038
- BioCyc:   LBOR355277:LBJ_1236-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25186; Mature: 25186

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMIRFRKKIPYLRYLEMQEKLRKFRRECILFLEHAPIITGGINYNPENLLLGKEFLESQ
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEHHHCCCEEECCCCCCCCCEEECHHHHHHC
GIQVHFVQRGGDFTAHEPGQLVIYPHVDLKKRNLPIRFYLENLLQSVIDSARTTWGLNLI
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEECEEEE
TDSDSPGLYLESNSSKKICSIGVNFKSFFTSHGVAFNLNNDFTAFRCINPCGRNWTDMTS
ECCCCCCEEEECCCCCEEEEECCCHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCHHH
VEKLGLDSGFAKRNQFISFMKANLNSFLGPISKLTHTVI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMIRFRKKIPYLRYLEMQEKLRKFRRECILFLEHAPIITGGINYNPENLLLGKEFLESQ
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHEEHHHCCCEEECCCCCCCCCEEECHHHHHHC
GIQVHFVQRGGDFTAHEPGQLVIYPHVDLKKRNLPIRFYLENLLQSVIDSARTTWGLNLI
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEECEEEE
TDSDSPGLYLESNSSKKICSIGVNFKSFFTSHGVAFNLNNDFTAFRCINPCGRNWTDMTS
ECCCCCCEEEECCCCCEEEEECCCHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCHHH
VEKLGLDSGFAKRNQFISFMKANLNSFLGPISKLTHTVI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA