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The map label for this gene is aspS [H]

Identifier: 116327961

GI number: 116327961

Start: 1470641

End: 1472455

Strand: Direct

Name: aspS [H]

Synonym: LBL_1250

Alternate gene names: 116327961

Gene position: 1470641-1472455 (Clockwise)

Preceding gene: 116327960

Following gene: 116327962

Centisome position: 40.69

GC content: 44.13

Gene sequence:

>1815_bases
ATGAGGAAACGGAATTCATTGGAACAGCAAAACTATAAAAATCGTTCTTGGGCCGGAGAATTAAACGAGTCCCAAGTAGG
TAAAAAAATCTTTCTTTACGGCTGGTCGTTCCGTTTCCGGGATCAGGGAGGAGTGATCTTTATAGATCTCCGTGACAGAA
CCGGTATCATTCAAGTCGTTGCACGTAAAGAGCTTTTGGGAGAAGAGTTCTTGATCGCCGAGAAGGTTCGTTCCGAATAC
GTTCTGGCGGTAAGCGGCACACTCAAAAAAAGAGACGCTGAATCCGTCAATTTGAAAATGCAAACCGGAACGATTGAAGT
CGTTTTAGATAAATTAGAAATTTTGAATATTGCCAAAACTCCTCCGTTTTCGTTGGACGAGTTCGACGAAGTTTCCGAAG
AACTCAGGCTTAAATATCGTTATCTGGATTTTAGAAGAGAAGAACTCAAAAATAGGATGATCAAAAGACATGAGTTTATA
TTCGCAATTCGTAATTATCTGAGTAAACGTAAGTTCGTCGAAATCGAAACTCCTATCTTAAACAAATCCACTCCCGAAGG
AGCGCGCGACTTTCTCGTTCCTTCCCGCCTCAACCCGAACCAATTTTACGCGTTACCACAGTCTCCTCAAATTTTCAAAC
AGATTCTGATGGTGGGAGGAATGGAGCGTTACTTCCAAATCGTAAAGTGTTTTCGGGACGAAGACTTACGTGCGGACAGG
CAGCCGGAATTTACCCAGCTCGATATGGAATTTTCTTTCGTTAATCAGGAAGAAATCCTTTCCGAGATAGAAGGTCTTGT
TTCCACGATTTATAAGGAAGTTTTTAATATTCAGCTTACGACTCCTTTCCCGAGAATGACCTACAAAACCGCAATCGAAG
AATACGGCTCGGACAAACCGGATCTTCGTTTCGGAATGAAACTCGTGGACGTTTCGGAAATCGTAAAAGACTGCGACTTC
AACGTATTTTCCGGAGCAGTGAAAAGCGGAGGAACCGTGAAGGCGATTTGTGTTCCCGGTGGTTCGGTAGTTTCCAGAAA
GGAAATCGAGGATTATACCGCATGGTTAAACCGAGACTACAAAGCTAAGGGTCTCGCGTATATGAAACACGGAAGTGAAG
GTTTGGAATCCACGATCACAAAACGTTTTAAAAAGGAAGAATTAGACGCCATCTCCAAAGTGTGCGGCTCCAAAGAAGGA
GATATGCTCTTTTTCGGAGCGGATGAAAAGGAGATCGTTAATCACTCGTTAGGCGCTCTTCGTCTGAAACTTTCCGAACG
TTTTGAAACTCCGAAACAAAATGAAATCAATATCACTTGGATCGTGGACTTTCCTATGTTCGAGTGGAACAAGGACCACA
AACGTTGGGACGCTCTGCATCACCCGTTCACTTCTCCTTCGGACGAAAGTATTCCGTTTTTTGAATCCATGGAAACTCTC
CAAAAAAACGCAAGTAAAGCGACCGCGAAAGCGTACGATCTTGTAATGAACGGAGTCGAAATCGGAGGTGGTTCCATCAG
GATTCATTCCCGCGAAATTCAAAACCAAGTATTCCGAGTTCTCGGAATCGGCGAAGAGGAAGCCCGGGAAAAATTCGGAT
TTTTGCTCGAGGCCCTTGAATTCGGAGCTCCGCCTCACGGAGGTTTGGCGTTCGGTATCGATCGAATGCTGATGCTCCTT
ACCGAAGGAAAATCGATTCGGGACGTGATCGCTTTCCCTAAAACTCAAAGAGGTCTTTGTCTTATGAGTGAATGTCCTTC
TCCCGTCGAGGAAAAACAGCTTCAAGAGTTGAAGATCAAACTGGTAAAGGTATAA

Upstream 100 bases:

>100_bases
GGAAGGCGGAAATTATCATCGCCAAAAACCGCTCCGGTCCTATCGGTTCTTTTCATCTTGCCTTTAGGCCCGAGCTTAGC
AGATTTGATAATATAGATTA

Downstream 100 bases:

>100_bases
ATTGCGCAAAGAACAGTTCAACTTCGAGAACCAGGATCTGTATCGTAAAATCTGCGGAATCAATGACGAAGGTGTTAAAA
TTCTCGAAAAACAACTCGAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MRKRNSLEQQNYKNRSWAGELNESQVGKKIFLYGWSFRFRDQGGVIFIDLRDRTGIIQVVARKELLGEEFLIAEKVRSEY
VLAVSGTLKKRDAESVNLKMQTGTIEVVLDKLEILNIAKTPPFSLDEFDEVSEELRLKYRYLDFRREELKNRMIKRHEFI
FAIRNYLSKRKFVEIETPILNKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADR
QPEFTQLDMEFSFVNQEEILSEIEGLVSTIYKEVFNIQLTTPFPRMTYKTAIEEYGSDKPDLRFGMKLVDVSEIVKDCDF
NVFSGAVKSGGTVKAICVPGGSVVSRKEIEDYTAWLNRDYKAKGLAYMKHGSEGLESTITKRFKKEELDAISKVCGSKEG
DMLFFGADEKEIVNHSLGALRLKLSERFETPKQNEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETL
QKNASKATAKAYDLVMNGVEIGGGSIRIHSREIQNQVFRVLGIGEEEAREKFGFLLEALEFGAPPHGGLAFGIDRMLMLL
TEGKSIRDVIAFPKTQRGLCLMSECPSPVEEKQLQELKIKLVKV

Sequences:

>Translated_604_residues
MRKRNSLEQQNYKNRSWAGELNESQVGKKIFLYGWSFRFRDQGGVIFIDLRDRTGIIQVVARKELLGEEFLIAEKVRSEY
VLAVSGTLKKRDAESVNLKMQTGTIEVVLDKLEILNIAKTPPFSLDEFDEVSEELRLKYRYLDFRREELKNRMIKRHEFI
FAIRNYLSKRKFVEIETPILNKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADR
QPEFTQLDMEFSFVNQEEILSEIEGLVSTIYKEVFNIQLTTPFPRMTYKTAIEEYGSDKPDLRFGMKLVDVSEIVKDCDF
NVFSGAVKSGGTVKAICVPGGSVVSRKEIEDYTAWLNRDYKAKGLAYMKHGSEGLESTITKRFKKEELDAISKVCGSKEG
DMLFFGADEKEIVNHSLGALRLKLSERFETPKQNEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETL
QKNASKATAKAYDLVMNGVEIGGGSIRIHSREIQNQVFRVLGIGEEEAREKFGFLLEALEFGAPPHGGLAFGIDRMLMLL
TEGKSIRDVIAFPKTQRGLCLMSECPSPVEEKQLQELKIKLVKV
>Mature_604_residues
MRKRNSLEQQNYKNRSWAGELNESQVGKKIFLYGWSFRFRDQGGVIFIDLRDRTGIIQVVARKELLGEEFLIAEKVRSEY
VLAVSGTLKKRDAESVNLKMQTGTIEVVLDKLEILNIAKTPPFSLDEFDEVSEELRLKYRYLDFRREELKNRMIKRHEFI
FAIRNYLSKRKFVEIETPILNKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADR
QPEFTQLDMEFSFVNQEEILSEIEGLVSTIYKEVFNIQLTTPFPRMTYKTAIEEYGSDKPDLRFGMKLVDVSEIVKDCDF
NVFSGAVKSGGTVKAICVPGGSVVSRKEIEDYTAWLNRDYKAKGLAYMKHGSEGLESTITKRFKKEELDAISKVCGSKEG
DMLFFGADEKEIVNHSLGALRLKLSERFETPKQNEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETL
QKNASKATAKAYDLVMNGVEIGGGSIRIHSREIQNQVFRVLGIGEEEAREKFGFLLEALEFGAPPHGGLAFGIDRMLMLL
TEGKSIRDVIAFPKTQRGLCLMSECPSPVEEKQLQELKIKLVKV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=596, Percent_Identity=40.1006711409396, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI194272210, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI5031815, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI45439306, Length=265, Percent_Identity=26.4150943396226, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=47.8920741989882, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1790571, Length=282, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789256, Length=285, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=615, Percent_Identity=35.4471544715447, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=191, Percent_Identity=28.7958115183246, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=279, Percent_Identity=27.5985663082437, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325153, Length=651, Percent_Identity=35.0230414746544, Blast_Score=300, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6320242, Length=176, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=322, Percent_Identity=24.5341614906832, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=271, Percent_Identity=28.7822878228782, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584738, Length=592, Percent_Identity=37.5, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=309, Percent_Identity=24.5954692556634, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640849, Length=309, Percent_Identity=24.5954692556634, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19921528, Length=247, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 69482; Mature: 69482

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRNSLEQQNYKNRSWAGELNESQVGKKIFLYGWSFRFRDQGGVIFIDLRDRTGIIQVV
CCCCCCCHHHCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCCEEEEEECCCCCHHHHH
ARKELLGEEFLIAEKVRSEYVLAVSGTLKKRDAESVNLKMQTGTIEVVLDKLEILNIAKT
HHHHHHCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
PPFSLDEFDEVSEELRLKYRYLDFRREELKNRMIKRHEFIFAIRNYLSKRKFVEIETPIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
NKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADR
CCCCCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
QPEFTQLDMEFSFVNQEEILSEIEGLVSTIYKEVFNIQLTTPFPRMTYKTAIEEYGSDKP
CCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCC
DLRFGMKLVDVSEIVKDCDFNVFSGAVKSGGTVKAICVPGGSVVSRKEIEDYTAWLNRDY
CHHHCCCCCCHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
KAKGLAYMKHGSEGLESTITKRFKKEELDAISKVCGSKEGDMLFFGADEKEIVNHSLGAL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHE
RLKLSERFETPKQNEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETL
EEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
QKNASKATAKAYDLVMNGVEIGGGSIRIHSREIQNQVFRVLGIGEEEAREKFGFLLEALE
HHHHHHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FGAPPHGGLAFGIDRMLMLLTEGKSIRDVIAFPKTQRGLCLMSECPSPVEEKQLQELKIK
HCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHH
LVKV
EECC
>Mature Secondary Structure
MRKRNSLEQQNYKNRSWAGELNESQVGKKIFLYGWSFRFRDQGGVIFIDLRDRTGIIQVV
CCCCCCCHHHCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCCEEEEEECCCCCHHHHH
ARKELLGEEFLIAEKVRSEYVLAVSGTLKKRDAESVNLKMQTGTIEVVLDKLEILNIAKT
HHHHHHCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
PPFSLDEFDEVSEELRLKYRYLDFRREELKNRMIKRHEFIFAIRNYLSKRKFVEIETPIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
NKSTPEGARDFLVPSRLNPNQFYALPQSPQIFKQILMVGGMERYFQIVKCFRDEDLRADR
CCCCCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
QPEFTQLDMEFSFVNQEEILSEIEGLVSTIYKEVFNIQLTTPFPRMTYKTAIEEYGSDKP
CCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCC
DLRFGMKLVDVSEIVKDCDFNVFSGAVKSGGTVKAICVPGGSVVSRKEIEDYTAWLNRDY
CHHHCCCCCCHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
KAKGLAYMKHGSEGLESTITKRFKKEELDAISKVCGSKEGDMLFFGADEKEIVNHSLGAL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHCCHHHE
RLKLSERFETPKQNEINITWIVDFPMFEWNKDHKRWDALHHPFTSPSDESIPFFESMETL
EEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
QKNASKATAKAYDLVMNGVEIGGGSIRIHSREIQNQVFRVLGIGEEEAREKFGFLLEALE
HHHHHHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FGAPPHGGLAFGIDRMLMLLTEGKSIRDVIAFPKTQRGLCLMSECPSPVEEKQLQELKIK
HCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHH
LVKV
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA