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The map label for this gene is tesA [H]

Identifier: 116327860

GI number: 116327860

Start: 1344358

End: 1344996

Strand: Direct

Name: tesA [H]

Synonym: LBL_1138

Alternate gene names: 116327860

Gene position: 1344358-1344996 (Clockwise)

Preceding gene: 116327856

Following gene: 116327861

Centisome position: 37.19

GC content: 41.0

Gene sequence:

>639_bases
ATGTTTCTTTTTAGGATCTTAATTTTTATTTCCAGTTGTTCTTTGTTTACTTCGATCCACGCAGAGTCGAATGTTCCTAA
GATTCGAATCCTGTTTTTCGGAGACAGTCTTACGGCTGGATTGGGGCTTGGTTCTCCGGAGGAGGCGTTTCCGGCTCTTG
TGGAAAAAGAACTTTTCAAGAGCGGAATTTCTACTCAAGCGATCAACGCCGGTATGAGCGGTGACACGACTTCGGGAGGT
CTTGCTCGTTTGGATTGGGCGATGTCATCCGGTTTTGATCTGTTCGTTTTAGAACTTGGAGCGAACGATTCTATGAGAGG
GATTTCTCCGGATCAAACCGAAAAAAATTTGAAAGAGATCATTGTTAGAGTAAAAAAGAAAAATCCGAAAGTGAAGATCC
TTCTTGTTGGTATGAAAACATTTCCGAATCTCGGGAAAGAGTACCGAAAAAAATTCGAAGCTGTTTTTCCTAAGATCGCA
AAGGAAGAAAATCTTCCACTCGTTCCTTTTTTTCTAAACGGAGTAGCGGGAGTCAAAAAACTCAATCAGAAGGACGGAAT
TCATCCGACTGCGGAAGGACATAGGATTCTCGCTAAAAATCTTTTTCCGTTCATTCAAAAAATTTTGAAGAAAGAATGA

Upstream 100 bases:

>100_bases
TAAGGAGCAGGAACACAATTAGAAAACGTCGGATCATAGTTTGACAGAATAGGAAAACCTTGAATTCTGTAAAAACAAAT
TCCGGGAGCAAACGATTGTT

Downstream 100 bases:

>100_bases
TTCTCCAAAAAGTGTGTTTACACTGAACTTTTACCGGAAATGATGGACCTACCTAAATGATCGCGGGAATAGCTCAGTGG
TAGAGCACCTCCTTGCCAAG

Product: lipase/acylhydrolase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MFLFRILIFISSCSLFTSIHAESNVPKIRILFFGDSLTAGLGLGSPEEAFPALVEKELFKSGISTQAINAGMSGDTTSGG
LARLDWAMSSGFDLFVLELGANDSMRGISPDQTEKNLKEIIVRVKKKNPKVKILLVGMKTFPNLGKEYRKKFEAVFPKIA
KEENLPLVPFFLNGVAGVKKLNQKDGIHPTAEGHRILAKNLFPFIQKILKKE

Sequences:

>Translated_212_residues
MFLFRILIFISSCSLFTSIHAESNVPKIRILFFGDSLTAGLGLGSPEEAFPALVEKELFKSGISTQAINAGMSGDTTSGG
LARLDWAMSSGFDLFVLELGANDSMRGISPDQTEKNLKEIIVRVKKKNPKVKILLVGMKTFPNLGKEYRKKFEAVFPKIA
KEENLPLVPFFLNGVAGVKKLNQKDGIHPTAEGHRILAKNLFPFIQKILKKE
>Mature_212_residues
MFLFRILIFISSCSLFTSIHAESNVPKIRILFFGDSLTAGLGLGSPEEAFPALVEKELFKSGISTQAINAGMSGDTTSGG
LARLDWAMSSGFDLFVLELGANDSMRGISPDQTEKNLKEIIVRVKKKNPKVKILLVGMKTFPNLGKEYRKKFEAVFPKIA
KEENLPLVPFFLNGVAGVKKLNQKDGIHPTAEGHRILAKNLFPFIQKILKKE

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=209, Percent_Identity=36.3636363636364, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23397; Mature: 23397

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLFRILIFISSCSLFTSIHAESNVPKIRILFFGDSLTAGLGLGSPEEAFPALVEKELFK
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHH
SGISTQAINAGMSGDTTSGGLARLDWAMSSGFDLFVLELGANDSMRGISPDQTEKNLKEI
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
IVRVKKKNPKVKILLVGMKTFPNLGKEYRKKFEAVFPKIAKEENLPLVPFFLNGVAGVKK
HHHHHCCCCCEEEEEEECHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LNQKDGIHPTAEGHRILAKNLFPFIQKILKKE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLFRILIFISSCSLFTSIHAESNVPKIRILFFGDSLTAGLGLGSPEEAFPALVEKELFK
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHH
SGISTQAINAGMSGDTTSGGLARLDWAMSSGFDLFVLELGANDSMRGISPDQTEKNLKEI
CCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
IVRVKKKNPKVKILLVGMKTFPNLGKEYRKKFEAVFPKIAKEENLPLVPFFLNGVAGVKK
HHHHHCCCCCEEEEEEECHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LNQKDGIHPTAEGHRILAKNLFPFIQKILKKE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]