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The map label for this gene is lexA

Identifier: 116327807

GI number: 116327807

Start: 1256005

End: 1256616

Strand: Reverse

Name: lexA

Synonym: LBL_1071

Alternate gene names: 116327807

Gene position: 1256616-1256005 (Counterclockwise)

Preceding gene: 116327810

Following gene: 116327806

Centisome position: 34.77

GC content: 44.77

Gene sequence:

>612_bases
ATGAAAGATTTGACAGATAAGCAACAGGCTGTCCTTGCGTTCATCACCACAATCATTAAGGAACGCGGTTTTCCTCCTAC
GATTCGAGAGATCGGAGACGAGTTCGGAATTACGGCGAAGGGCGCTTATGATCATCTTAAAGCGATTGAAAAGAAAGGAT
ATCTGAAGACCGCTAAGAACCAATCCAGGGCTATAGAACTAATTCGCCAGAGTCCGATGGAATCGATTCCTGTTCAGGCT
ACGAGTATTCCGGTCATTGGAAGGGTTGCAGCCGGTCTTCCGATCTTCGCAGACGAAAACATAGAATCATATATCCCGGT
CCCGGATGAGATGGCAAAGGGAAATGTTCCTATGTATGCTCTGAGAGTTCAGGGAGATTCCATGATTGAAGTCGGAATCG
ACAGTGGAGACATTGCGATCATTGAAAAGAGAGACATTGCTAGAAATGGAGAAATTGTAGTCGCTCTGATCGAAGACGAG
GCAACTTTGAAAGTATATTACAAAGAGCAGGATCAGATTCGTCTTGAGGCGAGAAATCCGAAATACAAACCGATCAAGAC
TAAGAAAGCGACTGTGATCGGCAAGCTAGTCGGTTTGTATCGAATTTACTGA

Upstream 100 bases:

>100_bases
TGAGACGGGTTTTTAGGTTGAATGCGCAATCTTTCAACTCTGTTTCGATTTTACTATTTTTGTGTTGACTTAGCATTTAT
TCACTACTAAGTTGTTTAGT

Downstream 100 bases:

>100_bases
TTGACAGAGGGACAAACTCGACTGAGACTTCGGACAGAGTGTTTTCACGAAATTTTTTCCTCTTTATCGTCCTACTTTTC
ATTGTTCAATGCAGTCCTCT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKDLTDKQQAVLAFITTIIKERGFPPTIREIGDEFGITAKGAYDHLKAIEKKGYLKTAKNQSRAIELIRQSPMESIPVQA
TSIPVIGRVAAGLPIFADENIESYIPVPDEMAKGNVPMYALRVQGDSMIEVGIDSGDIAIIEKRDIARNGEIVVALIEDE
ATLKVYYKEQDQIRLEARNPKYKPIKTKKATVIGKLVGLYRIY

Sequences:

>Translated_203_residues
MKDLTDKQQAVLAFITTIIKERGFPPTIREIGDEFGITAKGAYDHLKAIEKKGYLKTAKNQSRAIELIRQSPMESIPVQA
TSIPVIGRVAAGLPIFADENIESYIPVPDEMAKGNVPMYALRVQGDSMIEVGIDSGDIAIIEKRDIARNGEIVVALIEDE
ATLKVYYKEQDQIRLEARNPKYKPIKTKKATVIGKLVGLYRIY
>Mature_203_residues
MKDLTDKQQAVLAFITTIIKERGFPPTIREIGDEFGITAKGAYDHLKAIEKKGYLKTAKNQSRAIELIRQSPMESIPVQA
TSIPVIGRVAAGLPIFADENIESYIPVPDEMAKGNVPMYALRVQGDSMIEVGIDSGDIAIIEKRDIARNGEIVVALIEDE
ATLKVYYKEQDQIRLEARNPKYKPIKTKKATVIGKLVGLYRIY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=206, Percent_Identity=35.9223300970874, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_LEPBJ (Q04RH1)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801257.1
- ProteinModelPortal:   Q04RH1
- SMR:   Q04RH1
- STRING:   Q04RH1
- MEROPS:   S24.001
- GeneID:   4411399
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1985
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   VGVWRGY
- PhylomeDB:   Q04RH1
- ProtClustDB:   PRK00215
- BioCyc:   LBOR355277:LBJ_1985-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22605; Mature: 22605

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: ACT_SITE 127-127 ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLTDKQQAVLAFITTIIKERGFPPTIREIGDEFGITAKGAYDHLKAIEKKGYLKTAKN
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCHHHHHHHHHHCCCHHCCCC
QSRAIELIRQSPMESIPVQATSIPVIGRVAAGLPIFADENIESYIPVPDEMAKGNVPMYA
HHHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCEEECCCCCCCCCCCHHHHCCCCCEEE
LRVQGDSMIEVGIDSGDIAIIEKRDIARNGEIVVALIEDEATLKVYYKEQDQIRLEARNP
EEECCCCEEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEECCC
KYKPIKTKKATVIGKLVGLYRIY
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDLTDKQQAVLAFITTIIKERGFPPTIREIGDEFGITAKGAYDHLKAIEKKGYLKTAKN
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCHHHHHHHHHHCCCHHCCCC
QSRAIELIRQSPMESIPVQATSIPVIGRVAAGLPIFADENIESYIPVPDEMAKGNVPMYA
HHHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCEEECCCCCCCCCCCHHHHCCCCCEEE
LRVQGDSMIEVGIDSGDIAIIEKRDIARNGEIVVALIEDEATLKVYYKEQDQIRLEARNP
EEECCCCEEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEECCC
KYKPIKTKKATVIGKLVGLYRIY
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA