| Definition | Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008508 |
| Length | 3,614,446 |
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The map label for this gene is nadE [H]
Identifier: 116327703
GI number: 116327703
Start: 1096157
End: 1098091
Strand: Reverse
Name: nadE [H]
Synonym: LBL_0949
Alternate gene names: 116327703
Gene position: 1098091-1096157 (Counterclockwise)
Preceding gene: 116327706
Following gene: 116327701
Centisome position: 30.38
GC content: 43.72
Gene sequence:
>1935_bases ATGCAACCCGTGAGGCTTACGTCCGTTTCTCTCAAAACAAGAGTATTCGATTTTACAGGAAATTTAAATAAAATCAAAAG AGTTCTCGAACAGGAAAAGAACTCGGATCTGATCTTATTTCCGGAACTTTGTATTTCAGGTTACGGATGCGAAGATTCTT TTTTCTTTCCCCGAATCTGGAAAAAATCCTGGAATTCTTTGACCGAACTTCTTCCTCTTACTAAAAATAAGATCGTAGTC GTCGGCTTACCTATATTCCAAAATCCTTATCTATTCAATTGTGCGGCCGTACTTTGCAACGGAGTCGTAGCGGGAATCGT TCCCAAATCCAATCTCGCTTCCACGGGAGTGCACTACGAAAATAGATGGTTTACGAGAGGAGAAGAAACCCGAAAAAATT TTGCCGCTCCGGACGGTTCCGTAATTCCCTTCGGTTCCCTCATTTTTGAAACCGATCGGTTTTCTTTCGGAGTGGAAATT TGCGAAGATTCCTGGGTCCTACAGAAGCCTTCGATTCCACTCGCCGAAGCCGGAACCGATCTCATCCTTTCTCCGGGAGC GTCTCATTTCGCGTTCGGAAAACAGAGAATCCGCAGACGGATCTTTCAGGAAAATTCCAGAAGAGAATCGAACGTATATT TATTTTCCAATCTCTGCGGAAACGAGTCCGGAAGGCTGATTTTCGAAGGCGGCTCGATGATCGTTCAAAACGGAAAGTTA ATATCGGAGTCGCAACGTCTGTTTTTCGGCGATTTCAATTTTTGTTCCAGTGAAATAGACTTTGAAACTTCCAGAGCCGA TCGCGCAAAAAACTTTCGTCCTTCCAGAAACCGTTTCAACCCAAAAAAATCCTTCGAAGAAAATCGAATCCATCTCGGTC TTAAATTTCCCAAAAGAACCTCAAAGATAAATCATCCGCTTCCGGAACCTTCCGTTTCTCAAGAAGAAGAATCGTATTTG GATTTTACAAGAGCAGTAGCATTAGGACTTTTTGATTATCTAATAAACTCCAAAACAAAGGGTTACACACTTTCCCTTTC CGGCGGAGCGGATAGTTCTGTCTGCGCCCTTCTTGTGACTGCCATGAAAAAAATTGCCAAACAAGAACTCGGCGAAGACT TTTTCAACTCCCAAGGAATAGAGGAAGACTTCATTCTTTCGACTCTCTATCAAGCGACCGTAAACAACTCGGACCGGACT AGATCTCTTGCAAAGGCGCTGGCCGAGGACGTAAAATCCGTTCATGGAGAACTTACGATCGACACCGAAGTTCAAAATAT TTCCCAGAAAATTTCCGAGATTACGGGGATTTCTTTTAACTGGAACGAGCACAATCTAGTTCTGCAGAACATACAGGCGA GAATCCGTTCTCCGATCATTTGGATGCTCGCAAATTTAAACGGACATCTTCTTCTTTCCACCGGAAACAGAAGCGAAGCA AGCGTCGGTTATACTACAATGGACGGAGATTCTTCCGGATCGGTCGCTCCTCTTGCAGGAGTTAGTAAAGAATTTATTTT GAAATGGATGCGCTTTGTTGCGGAAGGAAGAGACTCGATCCTTCCCGCATATCCTTCTGTGAAAGAGATTGTACTTTCTC CTCCAAGCGCGGAACTGAAACCTCTGGAAGACGAACAAGAGGACGAAAAAGATCTAATGCCCTATCCACTTTTACAAAAA ATCGAAGAGTTATTCATAGTACGAGGCGCCGGTTTTTCCGAAATCGTTCAACTTCTTTCGAAAGATTCGGAAATACAAAA ATCAGTCTCCGGAGGGTTGGAAGAATCCGTGCGAAAATACATCGCCCTCTTCCACAGAAATCAGTGGAAACGAGAAAGGC TTCCGCCTTCGTTTCATCTAGACGATTACGGACTAGACCCTAAATCCAGCTTCCGGTTTCCGATTCTCTCCGAAGAAAAT GGATCAGGAATTTAG
Upstream 100 bases:
>100_bases TTCCAAGGAATTTCAAGTCTTATAAACAAGATCGTTTATCAAAATAAGTTCCAATAAAAAAACACTTTCAAAACAAATTT CAACCTGGATCCTGTAAAAC
Downstream 100 bases:
>100_bases AATATTCTAAATTGCCATCGCGTTCCGATTATCACCGACCGTTGCATAAATATAACGTCGCGAGATCTTACAATCCGGCT TGTTGAAAAGCGTTTTCCTT
Product: NAD(+) synthase (glutamine-hydrolyzing)
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]
Number of amino acids: Translated: 644; Mature: 644
Protein sequence:
>644_residues MQPVRLTSVSLKTRVFDFTGNLNKIKRVLEQEKNSDLILFPELCISGYGCEDSFFFPRIWKKSWNSLTELLPLTKNKIVV VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEETRKNFAAPDGSVIPFGSLIFETDRFSFGVEI CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRRIFQENSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL ISESQRLFFGDFNFCSSEIDFETSRADRAKNFRPSRNRFNPKKSFEENRIHLGLKFPKRTSKINHPLPEPSVSQEEESYL DFTRAVALGLFDYLINSKTKGYTLSLSGGADSSVCALLVTAMKKIAKQELGEDFFNSQGIEEDFILSTLYQATVNNSDRT RSLAKALAEDVKSVHGELTIDTEVQNISQKISEITGISFNWNEHNLVLQNIQARIRSPIIWMLANLNGHLLLSTGNRSEA SVGYTTMDGDSSGSVAPLAGVSKEFILKWMRFVAEGRDSILPAYPSVKEIVLSPPSAELKPLEDEQEDEKDLMPYPLLQK IEELFIVRGAGFSEIVQLLSKDSEIQKSVSGGLEESVRKYIALFHRNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEEN GSGI
Sequences:
>Translated_644_residues MQPVRLTSVSLKTRVFDFTGNLNKIKRVLEQEKNSDLILFPELCISGYGCEDSFFFPRIWKKSWNSLTELLPLTKNKIVV VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEETRKNFAAPDGSVIPFGSLIFETDRFSFGVEI CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRRIFQENSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL ISESQRLFFGDFNFCSSEIDFETSRADRAKNFRPSRNRFNPKKSFEENRIHLGLKFPKRTSKINHPLPEPSVSQEEESYL DFTRAVALGLFDYLINSKTKGYTLSLSGGADSSVCALLVTAMKKIAKQELGEDFFNSQGIEEDFILSTLYQATVNNSDRT RSLAKALAEDVKSVHGELTIDTEVQNISQKISEITGISFNWNEHNLVLQNIQARIRSPIIWMLANLNGHLLLSTGNRSEA SVGYTTMDGDSSGSVAPLAGVSKEFILKWMRFVAEGRDSILPAYPSVKEIVLSPPSAELKPLEDEQEDEKDLMPYPLLQK IEELFIVRGAGFSEIVQLLSKDSEIQKSVSGGLEESVRKYIALFHRNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEEN GSGI >Mature_644_residues MQPVRLTSVSLKTRVFDFTGNLNKIKRVLEQEKNSDLILFPELCISGYGCEDSFFFPRIWKKSWNSLTELLPLTKNKIVV VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEETRKNFAAPDGSVIPFGSLIFETDRFSFGVEI CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRRIFQENSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL ISESQRLFFGDFNFCSSEIDFETSRADRAKNFRPSRNRFNPKKSFEENRIHLGLKFPKRTSKINHPLPEPSVSQEEESYL DFTRAVALGLFDYLINSKTKGYTLSLSGGADSSVCALLVTAMKKIAKQELGEDFFNSQGIEEDFILSTLYQATVNNSDRT RSLAKALAEDVKSVHGELTIDTEVQNISQKISEITGISFNWNEHNLVLQNIQARIRSPIIWMLANLNGHLLLSTGNRSEA SVGYTTMDGDSSGSVAPLAGVSKEFILKWMRFVAEGRDSILPAYPSVKEIVLSPPSAELKPLEDEQEDEKDLMPYPLLQK IEELFIVRGAGFSEIVQLLSKDSEIQKSVSGGLEESVRKYIALFHRNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEEN GSGI
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0388
COG function: function code R; Predicted amidohydrolase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Homo sapiens, GI41393551, Length=674, Percent_Identity=28.9317507418398, Blast_Score=242, Evalue=8e-64, Organism=Caenorhabditis elegans, GI17538756, Length=690, Percent_Identity=29.7101449275362, Blast_Score=253, Evalue=2e-67, Organism=Saccharomyces cerevisiae, GI6321865, Length=678, Percent_Identity=26.2536873156342, Blast_Score=206, Evalue=8e-54, Organism=Drosophila melanogaster, GI24641841, Length=701, Percent_Identity=29.3865905848787, Blast_Score=246, Evalue=2e-65, Organism=Drosophila melanogaster, GI24641843, Length=701, Percent_Identity=29.3865905848787, Blast_Score=246, Evalue=2e-65,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 72377; Mature: 72377
Theoretical pI: Translated: 6.45; Mature: 6.45
Prosite motif: PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQPVRLTSVSLKTRVFDFTGNLNKIKRVLEQEKNSDLILFPELCISGYGCEDSFFFPRIW CCCCEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCHHHH KKSWNSLTELLPLTKNKIVVVGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYE HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC NRWFTRGEETRKNFAAPDGSVIPFGSLIFETDRFSFGVEICEDSWVLQKPSIPLAEAGTD CCCCCCCHHHHHHCCCCCCCCCCCCHHEEECCCCEECEEECCCCEEEECCCCCHHHCCCC LILSPGASHFAFGKQRIRRRIFQENSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCEEEECCCE ISESQRLFFGDFNFCSSEIDFETSRADRAKNFRPSRNRFNPKKSFEENRIHLGLKFPKRT ECCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEEEECCCHH SKINHPLPEPSVSQEEESYLDFTRAVALGLFDYLINSKTKGYTLSLSGGADSSVCALLVT HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH AMKKIAKQELGEDFFNSQGIEEDFILSTLYQATVNNSDRTRSLAKALAEDVKSVHGELTI HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEE DTEVQNISQKISEITGISFNWNEHNLVLQNIQARIRSPIIWMLANLNGHLLLSTGNRSEA EHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC SVGYTTMDGDSSGSVAPLAGVSKEFILKWMRFVAEGRDSILPAYPSVKEIVLSPPSAELK CCCEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCC PLEDEQEDEKDLMPYPLLQKIEELFIVRGAGFSEIVQLLSKDSEIQKSVSGGLEESVRKY CCCCCCCCHHCCCCCHHHHHHHHHHEECCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHH IALFHRNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEENGSGI HHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC >Mature Secondary Structure MQPVRLTSVSLKTRVFDFTGNLNKIKRVLEQEKNSDLILFPELCISGYGCEDSFFFPRIW CCCCEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCHHHH KKSWNSLTELLPLTKNKIVVVGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYE HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC NRWFTRGEETRKNFAAPDGSVIPFGSLIFETDRFSFGVEICEDSWVLQKPSIPLAEAGTD CCCCCCCHHHHHHCCCCCCCCCCCCHHEEECCCCEECEEECCCCEEEECCCCCHHHCCCC LILSPGASHFAFGKQRIRRRIFQENSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCEEEECCCE ISESQRLFFGDFNFCSSEIDFETSRADRAKNFRPSRNRFNPKKSFEENRIHLGLKFPKRT ECCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEEEECCCHH SKINHPLPEPSVSQEEESYLDFTRAVALGLFDYLINSKTKGYTLSLSGGADSSVCALLVT HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHH AMKKIAKQELGEDFFNSQGIEEDFILSTLYQATVNNSDRTRSLAKALAEDVKSVHGELTI HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEE DTEVQNISQKISEITGISFNWNEHNLVLQNIQARIRSPIIWMLANLNGHLLLSTGNRSEA EHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC SVGYTTMDGDSSGSVAPLAGVSKEFILKWMRFVAEGRDSILPAYPSVKEIVLSPPSAELK CCCEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCC PLEDEQEDEKDLMPYPLLQKIEELFIVRGAGFSEIVQLLSKDSEIQKSVSGGLEESVRKY CCCCCCCCHHCCCCCHHHHHHHHHHEECCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHH IALFHRNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEENGSGI HHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]