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The map label for this gene is algU [H]

Identifier: 116327662

GI number: 116327662

Start: 1055368

End: 1055991

Strand: Direct

Name: algU [H]

Synonym: LBL_0908

Alternate gene names: 116327662

Gene position: 1055368-1055991 (Clockwise)

Preceding gene: 116327661

Following gene: 116327663

Centisome position: 29.2

GC content: 40.71

Gene sequence:

>624_bases
ATGGATTCATCTTCTACAAAGATAGAGCAGAAACAAATTATTCCTAAAAAACCGACTTTAAGAGAGATTGAAATCGGTCT
TCTTAAAAAAATTAAGGAAGGGGATGACGAGGCTTATATTCAACTTGTCAATCCGTTTCGGGAGAGGCTTTATCGCAAAG
CGGTTTCGATGGTGAAGGACGGAGACGATGCGGAGGATATCGTTCAAGACGCGCTCATTTCCGGATACAGATCGATTCGT
AACTTTCGTGCGGATTCCGGGGTTTATACTTGGCTTTATCGAATCGTAGTTAATAAGTCCAAGGATATGTTAGCGAAACG
GAAACGAGCTAAAGAGAATTCCATGGATGATAAGGAATACCAGGTAACGGATGATCGGGTAGGATTCGAAAAAAAAATTG
AACTTAGTGATGAATCCAACTATCTAATTAGCAAAATCAACGGTTTGGAAGATCTCTATAAAGAGGTGATTGAACTTAGA
TACTTCGAGGAGATGTCTTACGCGCAGATCGCCGAAGTTCTGGGAACCAACGTGGGAACCGTAAAGAGTAGGCTCTTTAA
GGCGAAAGAATTCCTAAAACATTTGATCTTACAAGACGACAAGGGTGAAGGATACTTTAGGTAA

Upstream 100 bases:

>100_bases
GTTGGTTTTGATCAATCTTTCTTTGCAAGATCCCTAATTCTGTCCCGTCTATCCTAAGTTTCCGGATTGCCATATCCGCT
TTCTTTGGGATCTTTGAATC

Downstream 100 bases:

>100_bases
TAAAGATGGAACGTTTACAGTCAAAATTTAAAATTCCGCTTTTTCTTCGAAAGGAAGACGGAGATTTACTTAAAATTGAA
AATTCGCTCGTGAAAACGAT

Product: RNA polymerase sigma subunit

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MDSSSTKIEQKQIIPKKPTLREIEIGLLKKIKEGDDEAYIQLVNPFRERLYRKAVSMVKDGDDAEDIVQDALISGYRSIR
NFRADSGVYTWLYRIVVNKSKDMLAKRKRAKENSMDDKEYQVTDDRVGFEKKIELSDESNYLISKINGLEDLYKEVIELR
YFEEMSYAQIAEVLGTNVGTVKSRLFKAKEFLKHLILQDDKGEGYFR

Sequences:

>Translated_207_residues
MDSSSTKIEQKQIIPKKPTLREIEIGLLKKIKEGDDEAYIQLVNPFRERLYRKAVSMVKDGDDAEDIVQDALISGYRSIR
NFRADSGVYTWLYRIVVNKSKDMLAKRKRAKENSMDDKEYQVTDDRVGFEKKIELSDESNYLISKINGLEDLYKEVIELR
YFEEMSYAQIAEVLGTNVGTVKSRLFKAKEFLKHLILQDDKGEGYFR
>Mature_207_residues
MDSSSTKIEQKQIIPKKPTLREIEIGLLKKIKEGDDEAYIQLVNPFRERLYRKAVSMVKDGDDAEDIVQDALISGYRSIR
NFRADSGVYTWLYRIVVNKSKDMLAKRKRAKENSMDDKEYQVTDDRVGFEKKIELSDESNYLISKINGLEDLYKEVIELR
YFEEMSYAQIAEVLGTNVGTVKSRLFKAKEFLKHLILQDDKGEGYFR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=174, Percent_Identity=32.183908045977, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24140; Mature: 24140

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSSTKIEQKQIIPKKPTLREIEIGLLKKIKEGDDEAYIQLVNPFRERLYRKAVSMVKD
CCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GDDAEDIVQDALISGYRSIRNFRADSGVYTWLYRIVVNKSKDMLAKRKRAKENSMDDKEY
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCE
QVTDDRVGFEKKIELSDESNYLISKINGLEDLYKEVIELRYFEEMSYAQIAEVLGTNVGT
ECCCCCCCCHHEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VKSRLFKAKEFLKHLILQDDKGEGYFR
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDSSSTKIEQKQIIPKKPTLREIEIGLLKKIKEGDDEAYIQLVNPFRERLYRKAVSMVKD
CCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GDDAEDIVQDALISGYRSIRNFRADSGVYTWLYRIVVNKSKDMLAKRKRAKENSMDDKEY
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCE
QVTDDRVGFEKKIELSDESNYLISKINGLEDLYKEVIELRYFEEMSYAQIAEVLGTNVGT
ECCCCCCCCHHEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VKSRLFKAKEFLKHLILQDDKGEGYFR
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]