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The map label for this gene is miaB-1

Identifier: 116327643

GI number: 116327643

Start: 1030520

End: 1031869

Strand: Direct

Name: miaB-1

Synonym: LBL_0882

Alternate gene names: 116327643

Gene position: 1030520-1031869 (Clockwise)

Preceding gene: 116327639

Following gene: 116327644

Centisome position: 28.51

GC content: 44.52

Gene sequence:

>1350_bases
ATGGGCGTTCTCGAACGGGAAAAAAAGACAGGAAAAGTCTATATTGAGACATATGGCTGTCAAATGAATGAGTACGATTC
CGGAATCGTTTCCAGTTTGATGAAGGACGCCGAATATTCTTCTTCTCCCGATCCGGAGAATTCCGACATTATCTTTTTAA
ACACTTGTGCGATCCGGGAGAATGCCCACGCAAAAATTTACAATCGCCTTCAGTCACTCGGATATCTGAAAAAAAGGAAC
CCGGAACTTGTCATCGGAGTTCTAGGATGTATGGCTCAGAATTTAGGAGACGATCTTTTCCATCAGGAACTTCCCCTCGA
TTTGGTCGTAGGACCGGATAATTATAGAAGCCTTCCCGAACTGATTCAAAGAATTCGAAGTGGGGAACATTCCATCTCTC
TCACCCGTCTTTCAAAAATCGAAACCTACGACGAAATCGAACCAAGGGTCGTCAACGGAATCCAGGCCTTTGTCACTATT
ATGAGAGGTTGTAATAATTTTTGTACATTCTGCGTGGTTCCTTATACTCGAGGAAGGGAAAGAAGTAGGGATCCAAAAAG
TATCGTCCGAGAAATTCAAGACCTCACAGAGAAGGGAATTCGTCAGGTCACTTTACTGGGGCAGAATGTAAATTCTTACA
AAGAACAAGGAACCGACTTTGCCGGTTTGATTCAGATGCTCCTTGATGAGACTACTATCGAAAGAATCCGTTTTACTTCT
CCTCATCCGAAAGATTTTCCGGTTCGTCTTCTTCGGTTGATGGCGCAAAATCCTCGTTTTTGTCCGAATATCCATCTTCC
TCTGCAGGCGGGGAACACAAGAGTTCTGGAAGAAATGAAACGCTCCTATTCCAAGGAAGAATTTTTGGATGTGGTTCGAG
AAATCAGAAACATTGTTCCCGATGTCGGAATTACGACTGATATCATTGTGGGTTTTCCGAACGAAACTGAGGAAGAATTT
GAAGATACTCTTGCCGTGGTGCGTGAAGTGCAATTCGATATGGCGTTCATGTTCAAATATTCCGAAAGGGAAGGAACAAT
GGCCCGAAAGAAACTTCCCGACAATGTCTCCGAAGAGACGAAGTCAGCAAGACTTACAAAACTTGTGGATCTGCAGACTT
CGATTTCCCACGAACAAAATCGTGCTCGAATCGGAAGAGTATATTCGATTTTGATTGAGAACACCTCTCGAAAGTCGGAG
AAACAACTTTGCGGAAGAACTCCTTGCGGAAGGATGACCGTTTTTCCTCTTCCCGAGGGAAGGAGCGCTTCTGAGATGAT
CGGTTCTACGGTTTCCGTGCGGATCGAAAGCGCAACCAGTGCGACTTTGAAAGGGGGGATCCTTTCTTGA

Upstream 100 bases:

>100_bases
TTTGGTGTTCACTGCCATAACAATTTACAAGAAGGGGAGCACGGGTTCTATCTATGGAAAACCCCTTTACAGCTTGTCCT
GCAAGCAGAAATTCAGTTTT

Downstream 100 bases:

>100_bases
AGGATTCGAACAATCGAAAAAAAGTTTCCGCTAAAAAGCCTTCAAAGCTCACAAATAAAACGAATGTTCCCGAAATCGAT
CCTAAAACAAAAAAGGATCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRN
PELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTI
MRGCNNFCTFCVVPYTRGRERSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTS
PHPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITTDIIVGFPNETEEEF
EDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSE
KQLCGRTPCGRMTVFPLPEGRSASEMIGSTVSVRIESATSATLKGGILS

Sequences:

>Translated_449_residues
MGVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRN
PELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTI
MRGCNNFCTFCVVPYTRGRERSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTS
PHPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITTDIIVGFPNETEEEF
EDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSE
KQLCGRTPCGRMTVFPLPEGRSASEMIGSTVSVRIESATSATLKGGILS
>Mature_448_residues
GVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRNP
ELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTIM
RGCNNFCTFCVVPYTRGRERSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTSP
HPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITTDIIVGFPNETEEEFE
DTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSEK
QLCGRTPCGRMTVFPLPEGRSASEMIGSTVSVRIESATSATLKGGILS

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=40.546218487395, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=40.546218487395, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI93277076, Length=404, Percent_Identity=25.2475247524752, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=39.0134529147982, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=26.9736842105263, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=38.4449244060475, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI71996771, Length=306, Percent_Identity=25.4901960784314, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=40.5063291139241, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=28.2275711159737, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_LEPBJ (Q04UA3)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800275.1
- ProteinModelPortal:   Q04UA3
- SMR:   Q04UA3
- STRING:   Q04UA3
- GeneID:   4409765
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0869
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   Q04UA3
- ProtClustDB:   PRK14332
- BioCyc:   LBOR355277:LBJ_0869-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50907; Mature: 50776

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRE
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
NAHAKIYNRLQSLGYLKKRNPELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPE
CCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCHHHHHHCCCEEEEECCCCCHHHHH
LIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRE
HHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTS
CCCCHHHHHHHHHHHHHHHHHEEEHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
PHPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVP
CCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
DVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEET
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCHHHHH
KSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSEKQLCGRTPCGRMTVFPLPEG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCC
RSASEMIGSTVSVRIESATSATLKGGILS
CCHHHHHCCEEEEEEECCCCHHHCCCCCC
>Mature Secondary Structure 
GVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRE
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
NAHAKIYNRLQSLGYLKKRNPELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPE
CCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCHHHHHHCCCEEEEECCCCCHHHHH
LIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRE
HHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTS
CCCCHHHHHHHHHHHHHHHHHEEEHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
PHPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVP
CCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
DVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEET
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCHHHHH
KSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSEKQLCGRTPCGRMTVFPLPEG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCC
RSASEMIGSTVSVRIESATSATLKGGILS
CCHHHHHCCEEEEEEECCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA