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The map label for this gene is uvrD-3 [H]

Identifier: 116327571

GI number: 116327571

Start: 917869

End: 919893

Strand: Direct

Name: uvrD-3 [H]

Synonym: LBL_0802

Alternate gene names: 116327571

Gene position: 917869-919893 (Clockwise)

Preceding gene: 116327565

Following gene: 116327572

Centisome position: 25.39

GC content: 42.86

Gene sequence:

>2025_bases
ATGAAACTAAGCCCTGAACAGGAAAAAGCAGTTTGTCACGTGAACGGACCGCTCCTCATTTTTGCCGGAGCCGGTTCCGG
AAAAACTCGAGTTATCTCGAATCGAATCGCGCATTTAATCGAAAACGTGGGAGTTCCCGCAGGCAAAATCGTAGCACTTT
CTTTTACGAATAAGAGTGCCAGGGAAATGGAAGAACGGGTTCGAAAGATGATTCCGAAACAAAAACTCAAAGGAATTGTT
TTATCTACGTTTCATTCTCTGGGCTTGAACATACTCAAGAAACATATCGGATTTTTGGGATACAAACAACCTTTTCTATT
GATGAATCAGAACGATCAGGAAGGATTTTTAACTACATTATTGATTGCTAATAAAGTGGAATTGAAAAAAGCGAAAGTTT
CCGAAATACTCGGTAAGATATCTCGGATCAAAAACTCTGGGCCCGCTTACAGAGAATATCTGGATTCTTCGCTTTTGGAG
TCCGATCAGATCGCCAACCTAATTTACGACAGTTATCAGACTACGCTCAAAGAGCAGAACTCCCTCGATTTCGACGACCT
TATCCTTTTACCGGGAATATTGCTTAGGGATTTTGCGGAAGTCCGCGAAGAGTATCATAAGAAGTTTCAATACTTTATGG
TGGATGAGTTCCAAGATACAAACCAAACACAATACGTATTCTTAAGAGCTCTCATGGGAGAGAATCGGAATCTTTGTGTG
GTCGGAGACGACGATCAATCCATCTACGCATTTCGGGGATCGGATCTCAGCCTCATCCTGAATTTCGAGAAGGATTTTCC
CGAGGCGAACGTTGTGCGTCTATTGGAAAATTATAGATCGACTTCGGTGATCATTCAGGGGGCCAATTCGCTTATCAAAA
AGAATCTTTCCCGAAGGTCGAAAGAATTGTTCTCTTCCATTTCCGGAGGAAGAAAAATCCGTTATTTGGAAAGAATGGAC
GAGAAAGACGAGGCGGCTTACGTCGTGGATTGTATTCGGGAGGAAATTATCAAGGATGCGAGAGTGGGAAGTCAGATCGC
GATTTTATTCAGGACCAATTTTCAAACGCGGCCTTTCGAGGAGGAATTAAGAAGCAGATCCATACCTTATAAACTCGTGG
GAGGATATAATTTTTTCGATCGAAAAGAAGTTCGGGATATGATTTCCTACATCCGATTGATTGCGAATACAAGGGACGAC
GCTTCTTTACTTCGAATTTTGAATTATCCGAAGCGAGGAATCGGACCGGGAAGTGTTTCTTTGATTCACGAGAAGGCTTC
CCAAACGGGGGAATCCTTATACGAGATTTTATTCCGAATCTGCGAGTCCCCCGATTTTATTCCGAGTCTGCAAAAAAAAA
TTCAGTCCGAAATTTACAATTTTGTAAACCTCGTCGAGAGGACAAAAAAGAAATTCTCCACGACGCCTAAGATGTACTTT
GCGCTCCGGGAGTTCATTCAGGAAATAGGAATTGAAAAAGAGATTCTACTCGAAGAAAAGGACGAGAAGGTAGCCAAGGC
TCGGACTTTTAATCTTTCCGAACTCGTAAATATGATGTCTTACTTCGAAGAGAATCATGATTCTCCTGAAAAGCCGACGT
TATTCGATTTTATCAACCGGCTGAATTTACTCATGGAAGACGAGATTCCTTCCGATGAGGATAAGAAAGACAATCGGATA
CAACTTCTCACGATCCATCAATCGAAAGGTCTGGAATTCGAATCGGTTTATGTCCCCGGTATGGAAGAGGGAATTCTCCC
TAACTCCAGAGTATTGACGGAGGAATCCTCGGTAGATGAGGAAAGACGTCTACTCTACGTGGCTATGACTCGTGCAAGGA
AGCATTTATGCTTGACAGGGGTCGCAAATCGACGCAAATTTGGGGAGCAAATTGCTACCCAGGCTTCCCGGTTCCTCATG
GAAATTGATCCGGAGACTTTAGATTGGGTTTCTAACGACGAGGTTCGACAGCAAGAGACTGAAGATTTCTTCGCCGAACT
TGAAAAATTGAAAACTGGATCTTAG

Upstream 100 bases:

>100_bases
CAAAGAGTGCGGGTCGTATTTTAGTGGTGAGGTTTGGAGAGAATTCCCGGATGTACAAGATTGACAGAATCTTTCCCCCT
CTCATTATGTCACATGTCTC

Downstream 100 bases:

>100_bases
GCATGACGTTGAATACAAAAATAATCACTTTGGTAATGGCTTTCTTTCTTTTATCTGTCTGTGCAAGCGGTCAGAAATCC
GAAGATTCCAATCTTTCTCT

Product: DNA/RNA helicase UvrD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MKLSPEQEKAVCHVNGPLLIFAGAGSGKTRVISNRIAHLIENVGVPAGKIVALSFTNKSAREMEERVRKMIPKQKLKGIV
LSTFHSLGLNILKKHIGFLGYKQPFLLMNQNDQEGFLTTLLIANKVELKKAKVSEILGKISRIKNSGPAYREYLDSSLLE
SDQIANLIYDSYQTTLKEQNSLDFDDLILLPGILLRDFAEVREEYHKKFQYFMVDEFQDTNQTQYVFLRALMGENRNLCV
VGDDDQSIYAFRGSDLSLILNFEKDFPEANVVRLLENYRSTSVIIQGANSLIKKNLSRRSKELFSSISGGRKIRYLERMD
EKDEAAYVVDCIREEIIKDARVGSQIAILFRTNFQTRPFEEELRSRSIPYKLVGGYNFFDRKEVRDMISYIRLIANTRDD
ASLLRILNYPKRGIGPGSVSLIHEKASQTGESLYEILFRICESPDFIPSLQKKIQSEIYNFVNLVERTKKKFSTTPKMYF
ALREFIQEIGIEKEILLEEKDEKVAKARTFNLSELVNMMSYFEENHDSPEKPTLFDFINRLNLLMEDEIPSDEDKKDNRI
QLLTIHQSKGLEFESVYVPGMEEGILPNSRVLTEESSVDEERRLLYVAMTRARKHLCLTGVANRRKFGEQIATQASRFLM
EIDPETLDWVSNDEVRQQETEDFFAELEKLKTGS

Sequences:

>Translated_674_residues
MKLSPEQEKAVCHVNGPLLIFAGAGSGKTRVISNRIAHLIENVGVPAGKIVALSFTNKSAREMEERVRKMIPKQKLKGIV
LSTFHSLGLNILKKHIGFLGYKQPFLLMNQNDQEGFLTTLLIANKVELKKAKVSEILGKISRIKNSGPAYREYLDSSLLE
SDQIANLIYDSYQTTLKEQNSLDFDDLILLPGILLRDFAEVREEYHKKFQYFMVDEFQDTNQTQYVFLRALMGENRNLCV
VGDDDQSIYAFRGSDLSLILNFEKDFPEANVVRLLENYRSTSVIIQGANSLIKKNLSRRSKELFSSISGGRKIRYLERMD
EKDEAAYVVDCIREEIIKDARVGSQIAILFRTNFQTRPFEEELRSRSIPYKLVGGYNFFDRKEVRDMISYIRLIANTRDD
ASLLRILNYPKRGIGPGSVSLIHEKASQTGESLYEILFRICESPDFIPSLQKKIQSEIYNFVNLVERTKKKFSTTPKMYF
ALREFIQEIGIEKEILLEEKDEKVAKARTFNLSELVNMMSYFEENHDSPEKPTLFDFINRLNLLMEDEIPSDEDKKDNRI
QLLTIHQSKGLEFESVYVPGMEEGILPNSRVLTEESSVDEERRLLYVAMTRARKHLCLTGVANRRKFGEQIATQASRFLM
EIDPETLDWVSNDEVRQQETEDFFAELEKLKTGS
>Mature_674_residues
MKLSPEQEKAVCHVNGPLLIFAGAGSGKTRVISNRIAHLIENVGVPAGKIVALSFTNKSAREMEERVRKMIPKQKLKGIV
LSTFHSLGLNILKKHIGFLGYKQPFLLMNQNDQEGFLTTLLIANKVELKKAKVSEILGKISRIKNSGPAYREYLDSSLLE
SDQIANLIYDSYQTTLKEQNSLDFDDLILLPGILLRDFAEVREEYHKKFQYFMVDEFQDTNQTQYVFLRALMGENRNLCV
VGDDDQSIYAFRGSDLSLILNFEKDFPEANVVRLLENYRSTSVIIQGANSLIKKNLSRRSKELFSSISGGRKIRYLERMD
EKDEAAYVVDCIREEIIKDARVGSQIAILFRTNFQTRPFEEELRSRSIPYKLVGGYNFFDRKEVRDMISYIRLIANTRDD
ASLLRILNYPKRGIGPGSVSLIHEKASQTGESLYEILFRICESPDFIPSLQKKIQSEIYNFVNLVERTKKKFSTTPKMYF
ALREFIQEIGIEKEILLEEKDEKVAKARTFNLSELVNMMSYFEENHDSPEKPTLFDFINRLNLLMEDEIPSDEDKKDNRI
QLLTIHQSKGLEFESVYVPGMEEGILPNSRVLTEESSVDEERRLLYVAMTRARKHLCLTGVANRRKFGEQIATQASRFLM
EIDPETLDWVSNDEVRQQETEDFFAELEKLKTGS

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=676, Percent_Identity=37.8698224852071, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI2367296, Length=648, Percent_Identity=33.9506172839506, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1787196, Length=408, Percent_Identity=26.4705882352941, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=720, Percent_Identity=28.1944444444444, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=710, Percent_Identity=21.830985915493, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77645; Mature: 77645

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSPEQEKAVCHVNGPLLIFAGAGSGKTRVISNRIAHLIENVGVPAGKIVALSFTNKSA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
REMEERVRKMIPKQKLKGIVLSTFHSLGLNILKKHIGFLGYKQPFLLMNQNDQEGFLTTL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
LIANKVELKKAKVSEILGKISRIKNSGPAYREYLDSSLLESDQIANLIYDSYQTTLKEQN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLDFDDLILLPGILLRDFAEVREEYHKKFQYFMVDEFQDTNQTQYVFLRALMGENRNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEE
VGDDDQSIYAFRGSDLSLILNFEKDFPEANVVRLLENYRSTSVIIQGANSLIKKNLSRRS
EECCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
KELFSSISGGRKIRYLERMDEKDEAAYVVDCIREEIIKDARVGSQIAILFRTNFQTRPFE
HHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
EELRSRSIPYKLVGGYNFFDRKEVRDMISYIRLIANTRDDASLLRILNYPKRGIGPGSVS
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHH
LIHEKASQTGESLYEILFRICESPDFIPSLQKKIQSEIYNFVNLVERTKKKFSTTPKMYF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALREFIQEIGIEKEILLEEKDEKVAKARTFNLSELVNMMSYFEENHDSPEKPTLFDFINR
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNLLMEDEIPSDEDKKDNRIQLLTIHQSKGLEFESVYVPGMEEGILPNSRVLTEESSVDE
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCH
ERRLLYVAMTRARKHLCLTGVANRRKFGEQIATQASRFLMEIDPETLDWVSNDEVRQQET
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
EDFFAELEKLKTGS
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKLSPEQEKAVCHVNGPLLIFAGAGSGKTRVISNRIAHLIENVGVPAGKIVALSFTNKSA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
REMEERVRKMIPKQKLKGIVLSTFHSLGLNILKKHIGFLGYKQPFLLMNQNDQEGFLTTL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHH
LIANKVELKKAKVSEILGKISRIKNSGPAYREYLDSSLLESDQIANLIYDSYQTTLKEQN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLDFDDLILLPGILLRDFAEVREEYHKKFQYFMVDEFQDTNQTQYVFLRALMGENRNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEE
VGDDDQSIYAFRGSDLSLILNFEKDFPEANVVRLLENYRSTSVIIQGANSLIKKNLSRRS
EECCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
KELFSSISGGRKIRYLERMDEKDEAAYVVDCIREEIIKDARVGSQIAILFRTNFQTRPFE
HHHHHHHCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
EELRSRSIPYKLVGGYNFFDRKEVRDMISYIRLIANTRDDASLLRILNYPKRGIGPGSVS
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHH
LIHEKASQTGESLYEILFRICESPDFIPSLQKKIQSEIYNFVNLVERTKKKFSTTPKMYF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALREFIQEIGIEKEILLEEKDEKVAKARTFNLSELVNMMSYFEENHDSPEKPTLFDFINR
HHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNLLMEDEIPSDEDKKDNRIQLLTIHQSKGLEFESVYVPGMEEGILPNSRVLTEESSVDE
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCH
ERRLLYVAMTRARKHLCLTGVANRRKFGEQIATQASRFLMEIDPETLDWVSNDEVRQQET
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHH
EDFFAELEKLKTGS
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]