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The map label for this gene is ptsN [H]

Identifier: 116327545

GI number: 116327545

Start: 881683

End: 882144

Strand: Direct

Name: ptsN [H]

Synonym: LBL_0774

Alternate gene names: 116327545

Gene position: 881683-882144 (Clockwise)

Preceding gene: 116327544

Following gene: 116327546

Centisome position: 24.39

GC content: 42.64

Gene sequence:

>462_bases
ATGAATCAACTATTGACTCTTCTGCGCCCCGAAACGATTATTTTTAATTTGGAATCCGGGACTAAAGAAGAAGTTATTTC
CAGGCTTTTGCAGAAAGCGGTCGATACACGTCAGATTGACGCCGAAAATAAGGAAGAAATTTTGGAGTCTCTTCTTGCCC
GCGAAAAGTCGATGTCCACAGGGATTGGAAGCGGGGTGGCGATTCCTCACTGTTCGGTCAATCTCGTAGACGAACTCAAA
TGTGTAATGGGACTCAACCCAAGCGGAATCGATTTCGATTCGATTGATCATCAACCGGTTCATATCTTTATTCTTCTTAT
CGTCCCGAAAACCAAATTTCAAGAACACATCAAAACTCTAGCACAGATCGCAAAAGCGCTAAACGTAAAAGAAGATAGGG
AAAAACTCATTCGTTCCGGATCCTTCGAGGAAATCCAAAAAGCTTTTTCTCGGAACGTATAG

Upstream 100 bases:

>100_bases
ATTTCGGACAATTAGAATAGCTCGTAATTGTAATTAGAATGATGATTGTGTATCGTAACAAATGTAGACGGAAATACCAA
ATTCACGGAGAGTTGGGAGT

Downstream 100 bases:

>100_bases
CTGTGAGGGGGGAAATCTCCAGGTTTTTTTTATTTCTTATCGCATTGATTTTTTTTTCCGTTCTATTCGGTCATCTTCGT
TCGTTGAACCAAGAGTATCT

Product: protein-N(pi)-phosphohistidine-sugar phosphotransferase, subunit A

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNQLLTLLRPETIIFNLESGTKEEVISRLLQKAVDTRQIDAENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDELK
CVMGLNPSGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV

Sequences:

>Translated_153_residues
MNQLLTLLRPETIIFNLESGTKEEVISRLLQKAVDTRQIDAENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDELK
CVMGLNPSGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
>Mature_153_residues
MNQLLTLLRPETIIFNLESGTKEEVISRLLQKAVDTRQIDAENKEEILESLLAREKSMSTGIGSGVAIPHCSVNLVDELK
CVMGLNPSGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTLAQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=103, Percent_Identity=38.8349514563107, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17164; Mature: 17164

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLLTLLRPETIIFNLESGTKEEVISRLLQKAVDTRQIDAENKEEILESLLAREKSMST
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GIGSGVAIPHCSVNLVDELKCVMGLNPSGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
AQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNQLLTLLRPETIIFNLESGTKEEVISRLLQKAVDTRQIDAENKEEILESLLAREKSMST
CCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GIGSGVAIPHCSVNLVDELKCVMGLNPSGIDFDSIDHQPVHIFILLIVPKTKFQEHIKTL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHH
AQIAKALNVKEDREKLIRSGSFEEIQKAFSRNV
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]