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The map label for this gene is cspR [C]

Identifier: 116327528

GI number: 116327528

Start: 859270

End: 859731

Strand: Reverse

Name: cspR [C]

Synonym: LBL_0757

Alternate gene names: 116327528

Gene position: 859731-859270 (Counterclockwise)

Preceding gene: 116327529

Following gene: 116327527

Centisome position: 23.79

GC content: 44.37

Gene sequence:

>462_bases
ATGCCCTTGCACATAGGTCTTTACAGACCGGAAATTCCTCCCAATACCGGAAACATCGCGAGACTCTGCGTTGCCTTGGG
AGCGGAACTTCATATCATAGGACAAGCATCCTTCGACCTTTCCGAAAAAGCGGCGCGTAGAGCCGGATTAGATTATTGGG
ATAAAGTGAAAATTTCATTCCATTCTTCTTTAGAAGAATATAAAGCGATCCTTCCATCCGGAACCGATCCATATCTGATT
TCAGTTTACGGCAAACGTTCTTACACCGACGTTCAGTACAAGGAAGGAGATGCTTTTTTGTTCGGAAATGAAACCTCCGG
AGTTCCTACTAAAATGAAAAATTCCTGGAGTGAAGAACGGATCTTAAAAATTCCGATGGAAGATTCCTCCCGCTGTTTGA
ATTTAAGCAATTCGGTCGCGGTGATTTCGTACGAAGCGATGCGCCAAATCCGATCCTGGTAA

Upstream 100 bases:

>100_bases
GACTACAGTAGTTACCTGAGTGCGGATATAAGCGGTTCTACGAGTTCGTTCGGAGCGCTCCTTCAAAACTCCTTGAACAA
AAATAAGAAAAGATGATTTC

Downstream 100 bases:

>100_bases
AAACGAATTTACACGTCCAGAATTCCACACCCGAATGGATAAAGTATGAGCTTCCACCTTTCCAGAGGAGAAATCGAAAA
ACAAATTCAGTCGATGCTTT

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPLHIGLYRPEIPPNTGNIARLCVALGAELHIIGQASFDLSEKAARRAGLDYWDKVKISFHSSLEEYKAILPSGTDPYLI
SVYGKRSYTDVQYKEGDAFLFGNETSGVPTKMKNSWSEERILKIPMEDSSRCLNLSNSVAVISYEAMRQIRSW

Sequences:

>Translated_153_residues
MPLHIGLYRPEIPPNTGNIARLCVALGAELHIIGQASFDLSEKAARRAGLDYWDKVKISFHSSLEEYKAILPSGTDPYLI
SVYGKRSYTDVQYKEGDAFLFGNETSGVPTKMKNSWSEERILKIPMEDSSRCLNLSNSVAVISYEAMRQIRSW
>Mature_152_residues
PLHIGLYRPEIPPNTGNIARLCVALGAELHIIGQASFDLSEKAARRAGLDYWDKVKISFHSSLEEYKAILPSGTDPYLIS
VYGKRSYTDVQYKEGDAFLFGNETSGVPTKMKNSWSEERILKIPMEDSSRCLNLSNSVAVISYEAMRQIRSW

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=42.3841059602649, Blast_Score=122, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17219; Mature: 17088

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHIGLYRPEIPPNTGNIARLCVALGAELHIIGQASFDLSEKAARRAGLDYWDKVKISF
CCEEECEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCHHEEEEEH
HSSLEEYKAILPSGTDPYLISVYGKRSYTDVQYKEGDAFLFGNETSGVPTKMKNSWSEER
HHCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCCCCCCHHHCCCCCCE
ILKIPMEDSSRCLNLSNSVAVISYEAMRQIRSW
EEECCCCCCCCEECCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
PLHIGLYRPEIPPNTGNIARLCVALGAELHIIGQASFDLSEKAARRAGLDYWDKVKISF
CEEECEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCHHEEEEEH
HSSLEEYKAILPSGTDPYLISVYGKRSYTDVQYKEGDAFLFGNETSGVPTKMKNSWSEER
HHCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCCCCCCHHHCCCCCCE
ILKIPMEDSSRCLNLSNSVAVISYEAMRQIRSW
EEECCCCCCCCEECCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]