Definition Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence.
Accession NC_008508
Length 3,614,446

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The map label for this gene is metG

Identifier: 116327392

GI number: 116327392

Start: 663398

End: 665512

Strand: Reverse

Name: metG

Synonym: LBL_0593

Alternate gene names: 116327392

Gene position: 665512-663398 (Counterclockwise)

Preceding gene: 116327396

Following gene: 116327391

Centisome position: 18.41

GC content: 40.85

Gene sequence:

>2115_bases
GTGAACTCTTCTTCCATTCAAAGAAAAATATTAGTTACTTCCGCACTTCCTTATGCAAACGGACCGATCCATTTAGGCCA
CGTATTGGAAGGTATTCAAACGGATATTTGGGTTCGCTTTCAAAAAGCGATCGGAAACGAATGTTATTTTTTCTGTGCGG
ACGATACGCATGGAACTCCCGTAATGTTAGCGGCACGCAAAGAGAAAATAACTCCGGAACAACTCATCGAAAGAGTCGGG
CAAGAACATTATACAGACTTAACCTCTTTCGGAATTAATTACGATAATTATGATTCCACACATTCAAAAGCGAATCAAGA
AATTTCCAAAGATATTTACCTGAAATTGAAAGAGAAAGGCCATATTTCGAAAAGAAGTATCGAACAAGCTTACTGTGAAA
AAGATCGGATGTTTCTCCCCGATCGTTTTATCAAAGGCACCTGTCCCAATTGTAACTCGAAAAATCAATACGGAGATAAT
TGCGAAGTTTGCGGGGCGACATACAATCCGAAAGATTTGATCGATTCCCACTGCACTTTATGCGGAACTCCTCCCGTCGT
CAAGAACTCGGATCATATCTTTTTTAAACTCGGAAACTTTCATAAAAAGACGGAACAATCGAATGTGGATTTCGATTTAC
AATCATGGATAGAAACAAGCGAAGCCGTTTCCGAATCGGAAGGAGTTAAGAAGAAATTAAAAGAATGGTTCGACGCAGGT
TTGCAAGACTGGGACATTTCTAGAGACGGGCCTTATTTCGGCTTTGAAATTCCAAGTGAGAAAAATAAATATTTTTACGT
TTGGTTAGACGCTCCGGTCGGCTACATGGCTTCCTCCAAAAACTTCTTTGAAAAGAATTTTCCGAACGAACCGAATAAGT
TCGATTCCTTTTGGAAGGATAAAAATTCGGAAATCGTCCACTTTATCGGCAAGGATATATTATACTTTCATACTTTATTT
TGGCCTGCGATGTTGGAAGGAAGCGGCTATAGATCGCCTTCTAAAATCCATGTCCACGGTTTTATCGGTGTGAACGGAGA
GAAGATGTCCAAATCTCGCGGCACTTTCATCAAAGCGAAGACGTTCGCGAAATTCTTGGATGCGGAACATCTTCGTTTTT
ATCTAGCCGCTAAATTAGGTCCCGGAATGGACGATATCGATCTTTCTTTTGACGACTTCGTAAATAAAGTCAACGCGGAT
CTAGTAGGGAACTTGATCAATTCTGTTTCTAGAGTCTCCACCACAATTTTGGATACATTGGATAGAACGTTAGGAACCGT
TTCCGAAGAAGGACTCGCGCTCCTTGAAGAAATTCTTACTCAACCGGTTAAAACAGGTACCAGAGACGACTCGATTCAAA
ACATTATAAAAACTGCATACGAACAGAGGAATTACGCGAAAGTTATGAGGGAAATCACCCGTTTGGGAGATCGGGTAAAT
CGTTACGTAAACGACAACGCCCCTTGGAAACTTATCAAAGAAAATCCGGAAAAGGCGAGGGAAATCGTGACGGCTGTTTT
GAATGCAAGCAGATTTCTCGCGATTTATCTCTATCCCGTTGTCCCGAAAATCTCGGAACAAATCTATAAGTTACTGAATT
TGAAAGGATCTCCCGAATTCAAAGATTTGGATAAATCTAGAATATTAGAAAAAACGAAAATAAATCCGTACGAAATGATC
ACCAAACGTGTGGACGAAAAGGCAATCAAAGTTATGTTGGAAGAAAATAAACAATCCGAACATCCGAAAAAAGAAGAAAT
TCCCAAATCCTCCAACAAAGAAGAAGGAATCGAAATTTCGATCGAAGAACTCTCTAAAGTGGAACTAAGAGTCGGCGAAA
TCGTGGAAGCGAAACCGGTCGAAGGAGCGGACAAACTCGTAAATGTAAAAGTGGATTTAGGCGAACTCGGGATTAAAAAC
GTTTTTGCGGGTATCAAAATCGCATATCAACCGGAGAATCTCAAGGGTTTGAAAGTTGTCGTTGTCGCCAACCTGAAACC
GAGAAAGATGAAGTTCGGGATTTCAGAGGCGATGTTACTCGCTTCCGGCGAAGGAGAATCCTTGAGTTTATTCGTTCCTC
ACAAGGACGCTAAACCGGGAGATCGATTGAAATAA

Upstream 100 bases:

>100_bases
TCCAGGTTTTCAGAAAATTCTATCCGAACTATTGATTTTGCAATGAAAATCCGAAAAGAAAGACGTTGATTTTGACCTAA
ATTTTAAGAACCTGACTTTT

Downstream 100 bases:

>100_bases
ACGGTCGTCCGTTCTGCAAGAATATAGCTTCCATCTTCCATCAGTTAGGGTAATCCGGGATTGAAAATAAAACGATCTAT
AAGAACTTGCATTTTCAATT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MNSSSIQRKILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEKITPEQLIERVG
QEHYTDLTSFGINYDNYDSTHSKANQEISKDIYLKLKEKGHISKRSIEQAYCEKDRMFLPDRFIKGTCPNCNSKNQYGDN
CEVCGATYNPKDLIDSHCTLCGTPPVVKNSDHIFFKLGNFHKKTEQSNVDFDLQSWIETSEAVSESEGVKKKLKEWFDAG
LQDWDISRDGPYFGFEIPSEKNKYFYVWLDAPVGYMASSKNFFEKNFPNEPNKFDSFWKDKNSEIVHFIGKDILYFHTLF
WPAMLEGSGYRSPSKIHVHGFIGVNGEKMSKSRGTFIKAKTFAKFLDAEHLRFYLAAKLGPGMDDIDLSFDDFVNKVNAD
LVGNLINSVSRVSTTILDTLDRTLGTVSEEGLALLEEILTQPVKTGTRDDSIQNIIKTAYEQRNYAKVMREITRLGDRVN
RYVNDNAPWKLIKENPEKAREIVTAVLNASRFLAIYLYPVVPKISEQIYKLLNLKGSPEFKDLDKSRILEKTKINPYEMI
TKRVDEKAIKVMLEENKQSEHPKKEEIPKSSNKEEGIEISIEELSKVELRVGEIVEAKPVEGADKLVNVKVDLGELGIKN
VFAGIKIAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFVPHKDAKPGDRLK

Sequences:

>Translated_704_residues
MNSSSIQRKILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEKITPEQLIERVG
QEHYTDLTSFGINYDNYDSTHSKANQEISKDIYLKLKEKGHISKRSIEQAYCEKDRMFLPDRFIKGTCPNCNSKNQYGDN
CEVCGATYNPKDLIDSHCTLCGTPPVVKNSDHIFFKLGNFHKKTEQSNVDFDLQSWIETSEAVSESEGVKKKLKEWFDAG
LQDWDISRDGPYFGFEIPSEKNKYFYVWLDAPVGYMASSKNFFEKNFPNEPNKFDSFWKDKNSEIVHFIGKDILYFHTLF
WPAMLEGSGYRSPSKIHVHGFIGVNGEKMSKSRGTFIKAKTFAKFLDAEHLRFYLAAKLGPGMDDIDLSFDDFVNKVNAD
LVGNLINSVSRVSTTILDTLDRTLGTVSEEGLALLEEILTQPVKTGTRDDSIQNIIKTAYEQRNYAKVMREITRLGDRVN
RYVNDNAPWKLIKENPEKAREIVTAVLNASRFLAIYLYPVVPKISEQIYKLLNLKGSPEFKDLDKSRILEKTKINPYEMI
TKRVDEKAIKVMLEENKQSEHPKKEEIPKSSNKEEGIEISIEELSKVELRVGEIVEAKPVEGADKLVNVKVDLGELGIKN
VFAGIKIAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFVPHKDAKPGDRLK
>Mature_704_residues
MNSSSIQRKILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTPVMLAARKEKITPEQLIERVG
QEHYTDLTSFGINYDNYDSTHSKANQEISKDIYLKLKEKGHISKRSIEQAYCEKDRMFLPDRFIKGTCPNCNSKNQYGDN
CEVCGATYNPKDLIDSHCTLCGTPPVVKNSDHIFFKLGNFHKKTEQSNVDFDLQSWIETSEAVSESEGVKKKLKEWFDAG
LQDWDISRDGPYFGFEIPSEKNKYFYVWLDAPVGYMASSKNFFEKNFPNEPNKFDSFWKDKNSEIVHFIGKDILYFHTLF
WPAMLEGSGYRSPSKIHVHGFIGVNGEKMSKSRGTFIKAKTFAKFLDAEHLRFYLAAKLGPGMDDIDLSFDDFVNKVNAD
LVGNLINSVSRVSTTILDTLDRTLGTVSEEGLALLEEILTQPVKTGTRDDSIQNIIKTAYEQRNYAKVMREITRLGDRVN
RYVNDNAPWKLIKENPEKAREIVTAVLNASRFLAIYLYPVVPKISEQIYKLLNLKGSPEFKDLDKSRILEKTKINPYEMI
TKRVDEKAIKVMLEENKQSEHPKKEEIPKSSNKEEGIEISIEELSKVELRVGEIVEAKPVEGADKLVNVKVDLGELGIKN
VFAGIKIAYQPENLKGLKVVVVANLKPRKMKFGISEAMLLASGEGESLSLFVPHKDAKPGDRLK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=539, Percent_Identity=27.0871985157699, Blast_Score=212, Evalue=1e-54,
Organism=Homo sapiens, GI24308436, Length=551, Percent_Identity=25.0453720508167, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1788432, Length=709, Percent_Identity=46.8265162200282, Blast_Score=637, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=608, Percent_Identity=30.0986842105263, Blast_Score=269, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI212645096, Length=563, Percent_Identity=24.3339253996448, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71992781, Length=456, Percent_Identity=23.2456140350877, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=581, Percent_Identity=29.2598967297762, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321610, Length=572, Percent_Identity=22.5524475524476, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24655311, Length=547, Percent_Identity=27.9707495429616, Blast_Score=235, Evalue=8e-62,
Organism=Drosophila melanogaster, GI21358483, Length=533, Percent_Identity=24.577861163227, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_LEPBJ (Q04Q66)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801712.1
- ProteinModelPortal:   Q04Q66
- STRING:   Q04Q66
- GeneID:   4411632
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2519
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   Q04Q66
- ProtClustDB:   PRK00133
- BioCyc:   LBOR355277:LBJ_2519-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79882; Mature: 79882

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNSSSIQRKILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTP
CCCHHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
VMLAARKEKITPEQLIERVGQEHYTDLTSFGINYDNYDSTHSKANQEISKDIYLKLKEKG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHEEEECCCC
HISKRSIEQAYCEKDRMFLPDRFIKGTCPNCNSKNQYGDNCEVCGATYNPKDLIDSHCTL
CCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
CGTPPVVKNSDHIFFKLGNFHKKTEQSNVDFDLQSWIETSEAVSESEGVKKKLKEWFDAG
CCCCCCCCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LQDWDISRDGPYFGFEIPSEKNKYFYVWLDAPVGYMASSKNFFEKNFPNEPNKFDSFWKD
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHCCCCHHHCCCCCCCCHHHHHHCC
KNSEIVHFIGKDILYFHTLFWPAMLEGSGYRSPSKIHVHGFIGVNGEKMSKSRGTFIKAK
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHCCCCCEEEHH
TFAKFLDAEHLRFYLAAKLGPGMDDIDLSFDDFVNKVNADLVGNLINSVSRVSTTILDTL
HHHHHHHHHHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRTLGTVSEEGLALLEEILTQPVKTGTRDDSIQNIIKTAYEQRNYAKVMREITRLGDRVN
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYVNDNAPWKLIKENPEKAREIVTAVLNASRFLAIYLYPVVPKISEQIYKLLNLKGSPEF
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCH
KDLDKSRILEKTKINPYEMITKRVDEKAIKVMLEENKQSEHPKKEEIPKSSNKEEGIEIS
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCEEE
IEELSKVELRVGEIVEAKPVEGADKLVNVKVDLGELGIKNVFAGIKIAYQPENLKGLKVV
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHCCCEEEECCCCCCCEEEE
VVANLKPRKMKFGISEAMLLASGEGESLSLFVPHKDAKPGDRLK
EEECCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNSSSIQRKILVTSALPYANGPIHLGHVLEGIQTDIWVRFQKAIGNECYFFCADDTHGTP
CCCHHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
VMLAARKEKITPEQLIERVGQEHYTDLTSFGINYDNYDSTHSKANQEISKDIYLKLKEKG
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHEEEECCCC
HISKRSIEQAYCEKDRMFLPDRFIKGTCPNCNSKNQYGDNCEVCGATYNPKDLIDSHCTL
CCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
CGTPPVVKNSDHIFFKLGNFHKKTEQSNVDFDLQSWIETSEAVSESEGVKKKLKEWFDAG
CCCCCCCCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LQDWDISRDGPYFGFEIPSEKNKYFYVWLDAPVGYMASSKNFFEKNFPNEPNKFDSFWKD
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHCCCCHHHCCCCCCCCHHHHHHCC
KNSEIVHFIGKDILYFHTLFWPAMLEGSGYRSPSKIHVHGFIGVNGEKMSKSRGTFIKAK
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHCCCCCEEEHH
TFAKFLDAEHLRFYLAAKLGPGMDDIDLSFDDFVNKVNADLVGNLINSVSRVSTTILDTL
HHHHHHHHHHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRTLGTVSEEGLALLEEILTQPVKTGTRDDSIQNIIKTAYEQRNYAKVMREITRLGDRVN
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYVNDNAPWKLIKENPEKAREIVTAVLNASRFLAIYLYPVVPKISEQIYKLLNLKGSPEF
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCH
KDLDKSRILEKTKINPYEMITKRVDEKAIKVMLEENKQSEHPKKEEIPKSSNKEEGIEIS
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCEEE
IEELSKVELRVGEIVEAKPVEGADKLVNVKVDLGELGIKNVFAGIKIAYQPENLKGLKVV
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCCHHHHHCCCEEEECCCCCCCEEEE
VVANLKPRKMKFGISEAMLLASGEGESLSLFVPHKDAKPGDRLK
EEECCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA