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The map label for this gene is pcm [H]

Identifier: 116327215

GI number: 116327215

Start: 473947

End: 474612

Strand: Reverse

Name: pcm [H]

Synonym: LBL_0403

Alternate gene names: 116327215

Gene position: 474612-473947 (Counterclockwise)

Preceding gene: 116327216

Following gene: 116327214

Centisome position: 13.13

GC content: 40.24

Gene sequence:

>666_bases
ATGTCTTTTTCATCTAAGATTTGTAATCCGATTCTTCGTGAAAAAGAGAGAAAGAATATGGTCGATTTCCAAATCGTTTC
TCGAGGAATTCGTAATAAAAAAGTTCTCTCTGCGATGCGTTCGATTCCAAGAGAATGTTTTGTACCTGATTCCCACGTTT
CGCAAGCGTATGACGACAAACCTTTACCGATCGGATATCATCAAACGATTTCACAACCGTTCATGGTCGCATGGATGTCT
CTGCTTCTTGAAATTCGAAAGGGCGATCGAATTTTCGAAATCGGTACCGGCTCCGGTTATCAAAGCGCCGTTTTAATTTT
TCTTGGAGCGAAGTTATTCTCCGTTGAGTTCTTTGAATCTCTTCATAAAATCGCGATTCAAAATTTAGAACTCTGGAATC
CGGGTTGTACTCGAACAAACCGTTTTGTTAGCGGAAATGCAACTCAAGTTCTCAAACCTGAACTTCAATTTGATAAGATG
ATTTCCTGCGCCGCTCTTCCCGATCTTCCCGGCGTGGAAAGTTCCTACTTTCACTCCCTGGTCCCGGGAGGTTTTTTTAT
TTTTCCTATGGGAAAGAAAAAACAATTTTTAATAACCGCAAGAAGGAATTGGAACGATTGGTCTTTTGAATCTAAGTGTG
AAGTGAAATTCGTTCCCTTACTTTAA

Upstream 100 bases:

>100_bases
AAGGCCGAACCCAGGAACAGCGTAGAAAAACAGTCTTCACTGTCAACGAAAAAGATCCGAAGCTACTCATTCTCCCCGGA
TCATTGTTAGGGAGAGATCC

Downstream 100 bases:

>100_bases
ACTTTTTTTCGGAATCCTACCTTCAAGTAACTTTTGATGCGAATGGTAAACTATCCGATGAGGCACGTCTTTTGTTATTC
TAAAATCCGCTATTCTGTTT

Product: L-isoaspartate protein carboxylmethyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSFSSKICNPILREKERKNMVDFQIVSRGIRNKKVLSAMRSIPRECFVPDSHVSQAYDDKPLPIGYHQTISQPFMVAWMS
LLLEIRKGDRIFEIGTGSGYQSAVLIFLGAKLFSVEFFESLHKIAIQNLELWNPGCTRTNRFVSGNATQVLKPELQFDKM
ISCAALPDLPGVESSYFHSLVPGGFFIFPMGKKKQFLITARRNWNDWSFESKCEVKFVPLL

Sequences:

>Translated_221_residues
MSFSSKICNPILREKERKNMVDFQIVSRGIRNKKVLSAMRSIPRECFVPDSHVSQAYDDKPLPIGYHQTISQPFMVAWMS
LLLEIRKGDRIFEIGTGSGYQSAVLIFLGAKLFSVEFFESLHKIAIQNLELWNPGCTRTNRFVSGNATQVLKPELQFDKM
ISCAALPDLPGVESSYFHSLVPGGFFIFPMGKKKQFLITARRNWNDWSFESKCEVKFVPLL
>Mature_220_residues
SFSSKICNPILREKERKNMVDFQIVSRGIRNKKVLSAMRSIPRECFVPDSHVSQAYDDKPLPIGYHQTISQPFMVAWMSL
LLEIRKGDRIFEIGTGSGYQSAVLIFLGAKLFSVEFFESLHKIAIQNLELWNPGCTRTNRFVSGNATQVLKPELQFDKMI
SCAALPDLPGVESSYFHSLVPGGFFIFPMGKKKQFLITARRNWNDWSFESKCEVKFVPLL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=201, Percent_Identity=38.3084577114428, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25235; Mature: 25104

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSSKICNPILREKERKNMVDFQIVSRGIRNKKVLSAMRSIPRECFVPDSHVSQAYDDK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHHHCCCC
PLPIGYHQTISQPFMVAWMSLLLEIRKGDRIFEIGTGSGYQSAVLIFLGAKLFSVEFFES
CCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LHKIAIQNLELWNPGCTRTNRFVSGNATQVLKPELQFDKMISCAALPDLPGVESSYFHSL
HHHHHHHCCCCCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHH
VPGGFFIFPMGKKKQFLITARRNWNDWSFESKCEVKFVPLL
CCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure 
SFSSKICNPILREKERKNMVDFQIVSRGIRNKKVLSAMRSIPRECFVPDSHVSQAYDDK
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCHHHCCCCHHHHHHCCCC
PLPIGYHQTISQPFMVAWMSLLLEIRKGDRIFEIGTGSGYQSAVLIFLGAKLFSVEFFES
CCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LHKIAIQNLELWNPGCTRTNRFVSGNATQVLKPELQFDKMISCAALPDLPGVESSYFHSL
HHHHHHHCCCCCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHH
VPGGFFIFPMGKKKQFLITARRNWNDWSFESKCEVKFVPLL
CCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA