Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 116327161

GI number: 116327161

Start: 399370

End: 399762

Strand: Reverse

Name: gcvH

Synonym: LBL_0339

Alternate gene names: 116327161

Gene position: 399762-399370 (Counterclockwise)

Preceding gene: 116327162

Following gene: 116327160

Centisome position: 11.06

GC content: 45.29

Gene sequence:

>393_bases
ATGGCAGAAACACAAGCTCCCGCAGGTTATCTCTTCTCGGAAAAACACGAATGGGTAAAAGTAGAAGGGGACACAGCCCT
TATCGGAATTTCGGATTTTGCTCAATCCGCTCTCGGTGATATTGTTTTTGTGGATCTTCCAAAATCGGGAAAAACCGTCA
AACAGTTCGAAACCTTCGGTACAATCGAATCAGTAAAAGCGGCGGAAGATTTATATGCGCCTGTCGGCGGAGAAGTAATC
GAATCCAACTCTGCCCTTTCCAAAAACCCCGGAGACGTAAACTCGAAACCATTCGATTCCTGGATGATTAAGGTGAAAGG
TTTCTCCACCTCCGAATTGGAGAAACTTTTGAGTCCCGAAAAATACAAAGCGCTCGTCGCCAAACTCGAATAA

Upstream 100 bases:

>100_bases
TTCAGATTGAAATTCGCGAGCAACCCAAACAAGCTATCATAACAACAAAGCCTTTTATTCCGGGCAGTATTAGAAAAAAT
TAAATCAGGAAAAACGAATC

Downstream 100 bases:

>100_bases
CATCAAAGTCTAATATACGGATAGAAACAGATGAATATGAACTCTACTCTCCAGAACCGAAACAGAACGAATCTTGAAAG
GGTAAGTACGGATCCTTTGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAETQAPAGYLFSEKHEWVKVEGDTALIGISDFAQSALGDIVFVDLPKSGKTVKQFETFGTIESVKAAEDLYAPVGGEVI
ESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELEKLLSPEKYKALVAKLE

Sequences:

>Translated_130_residues
MAETQAPAGYLFSEKHEWVKVEGDTALIGISDFAQSALGDIVFVDLPKSGKTVKQFETFGTIESVKAAEDLYAPVGGEVI
ESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELEKLLSPEKYKALVAKLE
>Mature_129_residues
AETQAPAGYLFSEKHEWVKVEGDTALIGISDFAQSALGDIVFVDLPKSGKTVKQFETFGTIESVKAAEDLYAPVGGEVIE
SNSALSKNPGDVNSKPFDSWMIKVKGFSTSELEKLLSPEKYKALVAKLE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=47.5, Blast_Score=119, Evalue=7e-28,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=48.3333333333333, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=47.2, Blast_Score=113, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=45.7627118644068, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=40.6779661016949, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=41.8604651162791, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=44.9152542372881, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_LEPBJ (Q04PM8)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801900.1
- ProteinModelPortal:   Q04PM8
- SMR:   Q04PM8
- STRING:   Q04PM8
- GeneID:   4410895
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2731
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   FTDSHEW
- PhylomeDB:   Q04PM8
- ProtClustDB:   PRK01202
- BioCyc:   LBOR355277:LBJ_2731-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14074; Mature: 13943

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETQAPAGYLFSEKHEWVKVEGDTALIGISDFAQSALGDIVFVDLPKSGKTVKQFETFG
CCCCCCCCCCEECCCCCEEEEECCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
TIESVKAAEDLYAPVGGEVIESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELEKLLSPE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCH
KYKALVAKLE
HHHHHHHCCC
>Mature Secondary Structure 
AETQAPAGYLFSEKHEWVKVEGDTALIGISDFAQSALGDIVFVDLPKSGKTVKQFETFG
CCCCCCCCCEECCCCCEEEEECCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
TIESVKAAEDLYAPVGGEVIESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELEKLLSPE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCH
KYKALVAKLE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA