Definition Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence.
Accession NC_008508
Length 3,614,446

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The map label for this gene is gltX

Identifier: 116327083

GI number: 116327083

Start: 306951

End: 308516

Strand: Direct

Name: gltX

Synonym: LBL_0256

Alternate gene names: 116327083

Gene position: 306951-308516 (Clockwise)

Preceding gene: 116327082

Following gene: 116327084

Centisome position: 8.49

GC content: 41.89

Gene sequence:

>1566_bases
ATGAATCAAAATAGAGAAGTTCGTACCCGGTTTGCACCGTCTCCTAGCGGATTTTTACACGTAGGCGGAGCGAGAACCGC
CCTTTTCAATTATTTATACGCAAAATCCCAAGGTGGTAAATTCATCTTAAGAATCGAAGACACGGATCAAAACAGATCCA
CCGAAGATTCTTTCAAAATTATATTAGAATCTTTGAAATGGTTGGGAGTCCGTTGGGACGAGGGTCCGGAGATAGGAGGA
GAATATGGTCCTTATATTCAGAGTCAACGTCTCGACATATACAAAGAATATACGGAAAAACTTCTGAAAGAAAAAAAGGC
CTATCGTTGTTTTTGTACACAAGAAGAATTGGAGGCAAAAAAGAAGCAAGCTGAGGCAATGGGAGTTCCTTACGTCTACG
ACGGACTACACGCAAACATGTCCGACGAGGAAGTGGAGGAAAAACTCAAACAAAAAATTCCGTATTCGATTCGATTCAAA
ACCCCTTCTAAAACTCTCATTGTCGACGACATCATTCAAGGAAAAGTAAAATTTGAAACCAAGTTGATCGGAGATTTTAT
CATCGTTAAGTCGGACGGATTTCCTTCCTACAACTATGCGGTCGTAGTTGACGACGCTCTCATGAAGATTACTCATGTGA
TTCGTGGGGTGGGCCATCTTTCCAACACTCCTAGACAAATCTTATTGTACGAAGCTTTAGATTATGAAATTCCCGAGTTT
GCACATGCATCCGAAATTGTAGGAATGGACGGAAAAAAACTTTCCAAAAGAGCAGGTGCGACTTCGATTCTCGCGTTTAG
AGATTTAGGATATCTTCCGGAAACATTTCGAAATTATATGGCTCTTCTCGGATGGACTTCCTCCGACGGAAGGGAATTTC
TTCCTGAAGGTGAACTCGAAAAAATTTTCGATGTACATCGCTGTTCCAAATCCCCTTCCACATTTGACGTATTCAAAAAA
CCGAAAGGTGGAGAAGACGAAGTCGTAACGAACTTTTCGGACTTAACTCAAATTGCGGAAGCAATGAACCCAAAATCGAA
ACTCAATTGGTTATCGAATCGAACGATCAGGGATTTGAAAATTTCCAAAGTATTAGAAAGTCTATTACCGTTCTTGAAAG
ATAGAAAGGATATTCCTGAGGAGGTGAAAAATATAAATAGCCCCGCGCTTACTTCTATAATCGAATCTATAAGAGTATAT
CTGGACAACCTGGCTCAGGCCCCGGATTACATTGCGGAATTCTTTGTAACGGACCTCAAAATCCAATCGCAGGAAGCAAC
CGGGTTTATGAAAGACGGGGACGGTCCGAAAGTAGTAAAGGAATTCTACGGAATACTGAAGAATTCCGATCCGAAGACCG
ATGAGAATTATAAGGACCTAATGTCCAAAGTCGGAGAAGTTACCGGGCAAAAAGGAAAAACTCTCTATATGCCGATCCGG
GTGGCGACTACCGGAAAATCGGTCGGGTTGGAGCTACCGATTTTATTTCCTCTTTTAGGGAAAGAAAAGCTCCTCCAGAG
AATTGAAAAAACCGCCAAAGAAGCGGGAATTCCGTTGTCGACTTGA

Upstream 100 bases:

>100_bases
TTTCATTTTACGACCTGGCCGTAAATAAACCATTTACCACAACGGCTAATTTCAAAAATTATCCTATCATAAAAATTTCT
AACGAGAAAAACCAATTCCC

Downstream 100 bases:

>100_bases
AAATCCGATATTTCGTTCAAATTGCCCACTTTTAAGTTGTTAATCGTCGGGACGTAACGGAAACAGAGATGAAGGATTCC
AAAAAATTATTGCTAGTAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MNQNREVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVRWDEGPEIGG
EYGPYIQSQRLDIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQAEAMGVPYVYDGLHANMSDEEVEEKLKQKIPYSIRFK
TPSKTLIVDDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALDYEIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSSDGREFLPEGELEKIFDVHRCSKSPSTFDVFKK
PKGGEDEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLKISKVLESLLPFLKDRKDIPEEVKNINSPALTSIIESIRVY
LDNLAQAPDYIAEFFVTDLKIQSQEATGFMKDGDGPKVVKEFYGILKNSDPKTDENYKDLMSKVGEVTGQKGKTLYMPIR
VATTGKSVGLELPILFPLLGKEKLLQRIEKTAKEAGIPLST

Sequences:

>Translated_521_residues
MNQNREVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVRWDEGPEIGG
EYGPYIQSQRLDIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQAEAMGVPYVYDGLHANMSDEEVEEKLKQKIPYSIRFK
TPSKTLIVDDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALDYEIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSSDGREFLPEGELEKIFDVHRCSKSPSTFDVFKK
PKGGEDEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLKISKVLESLLPFLKDRKDIPEEVKNINSPALTSIIESIRVY
LDNLAQAPDYIAEFFVTDLKIQSQEATGFMKDGDGPKVVKEFYGILKNSDPKTDENYKDLMSKVGEVTGQKGKTLYMPIR
VATTGKSVGLELPILFPLLGKEKLLQRIEKTAKEAGIPLST
>Mature_521_residues
MNQNREVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKIILESLKWLGVRWDEGPEIGG
EYGPYIQSQRLDIYKEYTEKLLKEKKAYRCFCTQEELEAKKKQAEAMGVPYVYDGLHANMSDEEVEEKLKQKIPYSIRFK
TPSKTLIVDDIIQGKVKFETKLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALDYEIPEF
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSSDGREFLPEGELEKIFDVHRCSKSPSTFDVFKK
PKGGEDEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLKISKVLESLLPFLKDRKDIPEEVKNINSPALTSIIESIRVY
LDNLAQAPDYIAEFFVTDLKIQSQEATGFMKDGDGPKVVKEFYGILKNSDPKTDENYKDLMSKVGEVTGQKGKTLYMPIR
VATTGKSVGLELPILFPLLGKEKLLQRIEKTAKEAGIPLST

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=512, Percent_Identity=32.6171875, Blast_Score=245, Evalue=9e-65,
Organism=Homo sapiens, GI4826960, Length=309, Percent_Identity=25.2427184466019, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI62241042, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788743, Length=519, Percent_Identity=34.6820809248555, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=33.2046332046332, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786895, Length=213, Percent_Identity=23.943661971831, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=306, Percent_Identity=41.5032679738562, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324540, Length=323, Percent_Identity=42.7244582043344, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6321192, Length=243, Percent_Identity=28.3950617283951, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=116, Percent_Identity=33.6206896551724, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24665321, Length=321, Percent_Identity=43.3021806853583, Blast_Score=269, Evalue=4e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_LEPBJ (Q04PF0)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801978.1
- STRING:   Q04PF0
- GeneID:   4412084
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2815
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MANKIRV
- PhylomeDB:   Q04PF0
- ProtClustDB:   PRK01406
- BioCyc:   LBOR355277:LBJ_2815-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58931; Mature: 58931

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNQNREVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKI
CCCCCCCHHEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
ILESLKWLGVRWDEGPEIGGEYGPYIQSQRLDIYKEYTEKLLKEKKAYRCFCTQEELEAK
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
KKQAEAMGVPYVYDGLHANMSDEEVEEKLKQKIPYSIRFKTPSKTLIVDDIIQGKVKFET
HHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHCCCCEEEE
KLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALDYEIPEF
EEEEEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCCH
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSSDGREFLPEGELE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
KIFDVHRCSKSPSTFDVFKKPKGGEDEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLK
HHHHHHHHCCCCCCHHHHCCCCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
ISKVLESLLPFLKDRKDIPEEVKNINSPALTSIIESIRVYLDNLAQAPDYIAEFFVTDLK
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHE
IQSQEATGFMKDGDGPKVVKEFYGILKNSDPKTDENYKDLMSKVGEVTGQKGKTLYMPIR
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
VATTGKSVGLELPILFPLLGKEKLLQRIEKTAKEAGIPLST
EEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQNREVRTRFAPSPSGFLHVGGARTALFNYLYAKSQGGKFILRIEDTDQNRSTEDSFKI
CCCCCCCHHEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
ILESLKWLGVRWDEGPEIGGEYGPYIQSQRLDIYKEYTEKLLKEKKAYRCFCTQEELEAK
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
KKQAEAMGVPYVYDGLHANMSDEEVEEKLKQKIPYSIRFKTPSKTLIVDDIIQGKVKFET
HHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHCCCCEEEE
KLIGDFIIVKSDGFPSYNYAVVVDDALMKITHVIRGVGHLSNTPRQILLYEALDYEIPEF
EEEEEEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHEEEEHHHCCCCCCH
AHASEIVGMDGKKLSKRAGATSILAFRDLGYLPETFRNYMALLGWTSSDGREFLPEGELE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHH
KIFDVHRCSKSPSTFDVFKKPKGGEDEVVTNFSDLTQIAEAMNPKSKLNWLSNRTIRDLK
HHHHHHHHCCCCCCHHHHCCCCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
ISKVLESLLPFLKDRKDIPEEVKNINSPALTSIIESIRVYLDNLAQAPDYIAEFFVTDLK
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHE
IQSQEATGFMKDGDGPKVVKEFYGILKNSDPKTDENYKDLMSKVGEVTGQKGKTLYMPIR
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
VATTGKSVGLELPILFPLLGKEKLLQRIEKTAKEAGIPLST
EEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA