Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 116327071

GI number: 116327071

Start: 293354

End: 294157

Strand: Reverse

Name: cobS [H]

Synonym: LBL_0244

Alternate gene names: 116327071

Gene position: 294157-293354 (Counterclockwise)

Preceding gene: 116327072

Following gene: 116327070

Centisome position: 8.14

GC content: 41.17

Gene sequence:

>804_bases
TTGATTCTCTCTATAATCCGAGAGGAGTGGAATCGTTTCTGCGCCTCTTGGACGTTCAGTACAAGACTTCCCATTCCTCC
TTTTTACGTTTATTCCGAATCTACCGCTTCTAGGTCTACACGTTATTTTCCTTTGGTAGGTTGGATCGTTTCGGTCAGGG
CATCCTTTTGTACGTATTTTCTTTCTTGGATTTTACCCGTTGAAATTTCTGTGGTTTTCGGAATGATTTTGGGTATTTTG
AGCACGGGCGGTCTTCACGAAGACGGATTCGCAGACGTTTGTGACGCGTTCGGCGGAGGTTGGAATAAGGAAAAAATTTT
AGAGATCATGAAAGACAGCAGAATCGGGACCTTCGGAGCCTTAGGATTATTTCTTGCATTGATCCTGAAATATCTTTTAC
TCGTAAAACTTTTTCAAGTTTCTCCCCGGATCTTCCTATTCACATCTTGGTTTGCACACGGAGCCAGTCGGTGGCTTGCT
TTGGTGCTAATGATGTTGATTCCTTATGTGGGGAATAACGACCTATCCAAGTCCAAGCCGATGGTAAAAAATTTATCCCT
TTTCGATTTCGTTCTTTCCACGTTCTTCGGATGTTTTCCTGCAGTTTACTTCGCATTCCGATACCAAAATCTGATTTCGA
ATATCCTTTTAGGTTTCGTTTTTGCATTCTTATTCATTCTTTATTTTCGAAACTACTTTAAGAAATGGATCGAGGGTTTT
ACGGGAGATTGTCTCGGATTCACACAACAAGGAACCGAACTTCTCTTTTATTTGGGAATGACGGTTTCATGGAGCTTTAT
TTAA

Upstream 100 bases:

>100_bases
AAGGAGCCGGATGTGCGATCGCGTTTCCGATTCTATTATCCGCGATCGCTTTCTTAAACGATATGGCTTCCTTTGAATCC
GCAAAAGTAGACCAAAAACT

Downstream 100 bases:

>100_bases
TTCGACACACAACTCCGGATGTTCCGCAAGGAACTTGTTACGGTCGTACGAACGTTTCTTTAGCCGCGGACTTTGAGTAC
GAGTTTCGTTCCATTCTTGG

Product: adenosylcobinamide-GDP ribazoletransferase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MILSIIREEWNRFCASWTFSTRLPIPPFYVYSESTASRSTRYFPLVGWIVSVRASFCTYFLSWILPVEISVVFGMILGIL
STGGLHEDGFADVCDAFGGGWNKEKILEIMKDSRIGTFGALGLFLALILKYLLLVKLFQVSPRIFLFTSWFAHGASRWLA
LVLMMLIPYVGNNDLSKSKPMVKNLSLFDFVLSTFFGCFPAVYFAFRYQNLISNILLGFVFAFLFILYFRNYFKKWIEGF
TGDCLGFTQQGTELLFYLGMTVSWSFI

Sequences:

>Translated_267_residues
MILSIIREEWNRFCASWTFSTRLPIPPFYVYSESTASRSTRYFPLVGWIVSVRASFCTYFLSWILPVEISVVFGMILGIL
STGGLHEDGFADVCDAFGGGWNKEKILEIMKDSRIGTFGALGLFLALILKYLLLVKLFQVSPRIFLFTSWFAHGASRWLA
LVLMMLIPYVGNNDLSKSKPMVKNLSLFDFVLSTFFGCFPAVYFAFRYQNLISNILLGFVFAFLFILYFRNYFKKWIEGF
TGDCLGFTQQGTELLFYLGMTVSWSFI
>Mature_267_residues
MILSIIREEWNRFCASWTFSTRLPIPPFYVYSESTASRSTRYFPLVGWIVSVRASFCTYFLSWILPVEISVVFGMILGIL
STGGLHEDGFADVCDAFGGGWNKEKILEIMKDSRIGTFGALGLFLALILKYLLLVKLFQVSPRIFLFTSWFAHGASRWLA
LVLMMLIPYVGNNDLSKSKPMVKNLSLFDFVLSTFFGCFPAVYFAFRYQNLISNILLGFVFAFLFILYFRNYFKKWIEGF
TGDCLGFTQQGTELLFYLGMTVSWSFI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=265, Percent_Identity=27.5471698113208, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 30635; Mature: 30635

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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CCCHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA