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The map label for this gene is paaJ-4 [H]

Identifier: 116327036

GI number: 116327036

Start: 249083

End: 250264

Strand: Direct

Name: paaJ-4 [H]

Synonym: LBL_0209

Alternate gene names: 116327036

Gene position: 249083-250264 (Clockwise)

Preceding gene: 116327035

Following gene: 116327037

Centisome position: 6.89

GC content: 47.46

Gene sequence:

>1182_bases
ATGGAAGAGGCAGTCATTCTGGATGGAATTAGAACTCCGTTCGGAAATTTTGGAGGTACTTTAAAGGACCTTAGTGCGGT
TGATTTGGGGGTTTTGGTTTCCAAGGCGATTTTGGAAAAAACGGGAGTTTCTCCGGATGGGATTGGAGAATCAATTTTTG
GAAACGTAATTCCGACCGGAAAAGAGGCGATTTATCTCACTCGTCATATTGGTCTGAAATCCGGTCTTCCGTTGGGGATT
CCGGCCTTGACTCTGAATCGTCTCTGTGGTTCCGGTATGGAAGCGATCATTCAGGCCGCAAAGAAGATTTACCTTGGAGA
TGCGGACGCGGTTCTTGCGGGTGGCGTGGAATCTATGAGCAATGCTCCTTATGTTGTGCGTAACGCACGTTGGGGAGTTC
GTTATGGCTCTGTTGAATTCGAGGATACGTTGGAGCAAGGTCTTACCGACCAATACGTAGGTTTGATCATGGGACAGACT
GCGGAAAATCTTGCGAATCAATATAAAATTTCCAGACAAGAACAAGACGAATGGGCTGCTATTTCTCAAACCCGTGCCGA
AAAAGCCACTGTGGAAGGTCGTCTTAAGGACGAGATTTTTGCCGTTACGATTCCCGGTAAAAAACCGATTACATTAGAAA
AAGATGAATTTATCAAAGGTGTTTCCGGAATTGATAAACTTGGAACTTTGAAAGCTGTTTTCCGGGATGGTGGAACTGTA
ACTGCGGGAAATTCATCCGGTTTGAATGACGGAGCCGCCGCTACGATCGTGACTTCCGCTTCTTACGCGAAGAAGATCGG
TAAAAAACCTCTCGCGATCATTCGCGGTTACGGTCACGCGGGCTGTGATCCTGCGAAGATGGGAATCGGTCCCGCACTTG
CGATTCCGGCCGCTTTGAAGAAAGCAGGTTTAAAACTTTCTGACATGAGCCTTGTAGAAGTCAACGAAGCTTTTGCCGCC
CAGTATCTTGCCGTTCAAAAGGAACTTGGTCTTAATCCGGAAATTACTAATGTAAACGGAGGGGCCGTCGCCATTGGGCA
TCCGCTTGGAGCTTCCGGCGCCAGAGTTACGATTACTCTAGCATACGAACTAAAAAGAAGAAAGGCGAAATACGGTGTTG
CTTCTCTTTGTATCGGAGGCGGCCAAGGGATTGCGCTCGTTTTGGAAAATCCGGAAGCGTAA

Upstream 100 bases:

>100_bases
TGGATATCTACAGATGTGATGTGTCGGATAACGTGTTGAAGTGCATTACAGAGCGAAAAAACATTCAATAATTAGAATAT
TAAATTTATTAGGAGTAATT

Downstream 100 bases:

>100_bases
CTAGAAGTTTTGCTTAATATTTTATCAAAAGGCTTGGAAAACTCCGGGCCTTTTTGTTATAGTTTGTATCAATCCAAATA
TTATATTTTTTAAATATTAT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MEEAVILDGIRTPFGNFGGTLKDLSAVDLGVLVSKAILEKTGVSPDGIGESIFGNVIPTGKEAIYLTRHIGLKSGLPLGI
PALTLNRLCGSGMEAIIQAAKKIYLGDADAVLAGGVESMSNAPYVVRNARWGVRYGSVEFEDTLEQGLTDQYVGLIMGQT
AENLANQYKISRQEQDEWAAISQTRAEKATVEGRLKDEIFAVTIPGKKPITLEKDEFIKGVSGIDKLGTLKAVFRDGGTV
TAGNSSGLNDGAAATIVTSASYAKKIGKKPLAIIRGYGHAGCDPAKMGIGPALAIPAALKKAGLKLSDMSLVEVNEAFAA
QYLAVQKELGLNPEITNVNGGAVAIGHPLGASGARVTITLAYELKRRKAKYGVASLCIGGGQGIALVLENPEA

Sequences:

>Translated_393_residues
MEEAVILDGIRTPFGNFGGTLKDLSAVDLGVLVSKAILEKTGVSPDGIGESIFGNVIPTGKEAIYLTRHIGLKSGLPLGI
PALTLNRLCGSGMEAIIQAAKKIYLGDADAVLAGGVESMSNAPYVVRNARWGVRYGSVEFEDTLEQGLTDQYVGLIMGQT
AENLANQYKISRQEQDEWAAISQTRAEKATVEGRLKDEIFAVTIPGKKPITLEKDEFIKGVSGIDKLGTLKAVFRDGGTV
TAGNSSGLNDGAAATIVTSASYAKKIGKKPLAIIRGYGHAGCDPAKMGIGPALAIPAALKKAGLKLSDMSLVEVNEAFAA
QYLAVQKELGLNPEITNVNGGAVAIGHPLGASGARVTITLAYELKRRKAKYGVASLCIGGGQGIALVLENPEA
>Mature_393_residues
MEEAVILDGIRTPFGNFGGTLKDLSAVDLGVLVSKAILEKTGVSPDGIGESIFGNVIPTGKEAIYLTRHIGLKSGLPLGI
PALTLNRLCGSGMEAIIQAAKKIYLGDADAVLAGGVESMSNAPYVVRNARWGVRYGSVEFEDTLEQGLTDQYVGLIMGQT
AENLANQYKISRQEQDEWAAISQTRAEKATVEGRLKDEIFAVTIPGKKPITLEKDEFIKGVSGIDKLGTLKAVFRDGGTV
TAGNSSGLNDGAAATIVTSASYAKKIGKKPLAIIRGYGHAGCDPAKMGIGPALAIPAALKKAGLKLSDMSLVEVNEAFAA
QYLAVQKELGLNPEITNVNGGAVAIGHPLGASGARVTITLAYELKRRKAKYGVASLCIGGGQGIALVLENPEA

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=382, Percent_Identity=52.0942408376963, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=43.3673469387755, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=41.2213740458015, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=40.3022670025189, Blast_Score=244, Evalue=7e-65,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=32.9466357308585, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=31.4578005115089, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI87082165, Length=394, Percent_Identity=43.9086294416244, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=45.9183673469388, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=42.3558897243108, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=41.5617128463476, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788683, Length=421, Percent_Identity=34.2042755344418, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=381, Percent_Identity=53.0183727034121, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=39.2307692307692, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=39.8457583547558, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=36, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=31.7948717948718, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=43.0730478589421, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=36.8686868686869, Blast_Score=206, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=45.7800511508951, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI17648125, Length=382, Percent_Identity=47.3821989528796, Blast_Score=323, Evalue=9e-89,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=43.4782608695652, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=34.037558685446, Blast_Score=188, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40964; Mature: 40964

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEAVILDGIRTPFGNFGGTLKDLSAVDLGVLVSKAILEKTGVSPDGIGESIFGNVIPTG
CCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
KEAIYLTRHIGLKSGLPLGIPALTLNRLCGSGMEAIIQAAKKIYLGDADAVLAGGVESMS
CCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHCCHHHHC
NAPYVVRNARWGVRYGSVEFEDTLEQGLTDQYVGLIMGQTAENLANQYKISRQEQDEWAA
CCCEEEECCCCCEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHH
ISQTRAEKATVEGRLKDEIFAVTIPGKKPITLEKDEFIKGVSGIDKLGTLKAVFRDGGTV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECHHHHHHHHCCHHHHHHHHHHHHCCCEE
TAGNSSGLNDGAAATIVTSASYAKKIGKKPLAIIRGYGHAGCDPAKMGIGPALAIPAALK
EECCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHH
KAGLKLSDMSLVEVNEAFAAQYLAVQKELGLNPEITNVNGGAVAIGHPLGASGARVTITL
HHCCEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCEEEEEE
AYELKRRKAKYGVASLCIGGGQGIALVLENPEA
EEHHHHHHHHCCCEEEEEECCCEEEEEEECCCC
>Mature Secondary Structure
MEEAVILDGIRTPFGNFGGTLKDLSAVDLGVLVSKAILEKTGVSPDGIGESIFGNVIPTG
CCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
KEAIYLTRHIGLKSGLPLGIPALTLNRLCGSGMEAIIQAAKKIYLGDADAVLAGGVESMS
CCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHCCHHHHC
NAPYVVRNARWGVRYGSVEFEDTLEQGLTDQYVGLIMGQTAENLANQYKISRQEQDEWAA
CCCEEEECCCCCEEECCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHH
ISQTRAEKATVEGRLKDEIFAVTIPGKKPITLEKDEFIKGVSGIDKLGTLKAVFRDGGTV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECHHHHHHHHCCHHHHHHHHHHHHCCCEE
TAGNSSGLNDGAAATIVTSASYAKKIGKKPLAIIRGYGHAGCDPAKMGIGPALAIPAALK
EECCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHH
KAGLKLSDMSLVEVNEAFAAQYLAVQKELGLNPEITNVNGGAVAIGHPLGASGARVTITL
HHCCEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCEEEEEE
AYELKRRKAKYGVASLCIGGGQGIALVLENPEA
EEHHHHHHHHCCCEEEEEECCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]