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The map label for this gene is folE

Identifier: 116326960

GI number: 116326960

Start: 147972

End: 148523

Strand: Reverse

Name: folE

Synonym: LBL_0126

Alternate gene names: 116326960

Gene position: 148523-147972 (Counterclockwise)

Preceding gene: 116326962

Following gene: 116326959

Centisome position: 4.11

GC content: 43.48

Gene sequence:

>552_bases
TTGGAAGAAAATATCGTAAACATTCTAAAATCGATCGGAGAAGATCCGACTAGAGAAGGTCTTCTCAACACTCCGAAACG
AGTGAAAAAAGCCTACGACTTTCTGACATCCGGCTACCACGTCGACATTACAAAAGTTGTCAACGGAGCGATCTTTGAAG
AATCTACGGAAGGAATGATCCTCGTTCGAGACATCGAAGTGTATTCTCTCTGTGAACATCATCTTCTTCCTTTTTACGGA
AGAGCTCATGTCGCTTATCTTCCTAATAAAAAGATCATCGGAATCAGCAAAATTCCTCGAATCGTCGATGTGTTTGCCAG
AAGACTTCAAGTTCAGGAACGTCTCACGGAACAAATCGCCTACGCCGTTCAAGAAGTTCTCGATCCCCAAGGAGTCGCGG
TGGTCATTAAGGCGAAACATCTTTGTATGATGATGAGGGGAGTCGAAAAACAAAATTCGGAACTTTTCACCTCCTGCCTG
TTAGGGGCTTTTAAAGAAAATATGGTAACCCGTTCCGAGTTTTTGGATCTCATTCGCACCGGATCTACTTGA

Upstream 100 bases:

>100_bases
AATGAGAAGTTAGATTTAAAGAAGCGATCCGGAATTCTCCGAAAAGAATCTAACAAAAAGTCTCCCGATTCAATCGGGAT
AATCGAATGAAGAGTTTAAT

Downstream 100 bases:

>100_bases
GTAATCCAACCTTCTTCGGGCGATTTTCCTAAGAGAAAGGTTCGGATTTCCTTTTCTGCAATTTGGTAGACAGAGAGAAA
AAAAAGAACATTCTTTGCGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MEENIVNILKSIGEDPTREGLLNTPKRVKKAYDFLTSGYHVDITKVVNGAIFEESTEGMILVRDIEVYSLCEHHLLPFYG
RAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAVQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCL
LGAFKENMVTRSEFLDLIRTGST

Sequences:

>Translated_183_residues
MEENIVNILKSIGEDPTREGLLNTPKRVKKAYDFLTSGYHVDITKVVNGAIFEESTEGMILVRDIEVYSLCEHHLLPFYG
RAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAVQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCL
LGAFKENMVTRSEFLDLIRTGST
>Mature_183_residues
MEENIVNILKSIGEDPTREGLLNTPKRVKKAYDFLTSGYHVDITKVVNGAIFEESTEGMILVRDIEVYSLCEHHLLPFYG
RAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAVQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCL
LGAFKENMVTRSEFLDLIRTGST

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=174, Percent_Identity=55.1724137931034, Blast_Score=219, Evalue=1e-57,
Organism=Homo sapiens, GI4503949, Length=174, Percent_Identity=55.1724137931034, Blast_Score=219, Evalue=1e-57,
Organism=Homo sapiens, GI66932970, Length=155, Percent_Identity=50.3225806451613, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI66932972, Length=132, Percent_Identity=54.5454545454545, Blast_Score=166, Evalue=8e-42,
Organism=Escherichia coli, GI1788476, Length=181, Percent_Identity=41.4364640883978, Blast_Score=120, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=172, Percent_Identity=53.4883720930233, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321706, Length=175, Percent_Identity=54.2857142857143, Blast_Score=208, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24656782, Length=173, Percent_Identity=52.0231213872832, Blast_Score=196, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24656791, Length=173, Percent_Identity=52.0231213872832, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24656796, Length=173, Percent_Identity=52.0231213872832, Blast_Score=195, Evalue=2e-50,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_LEPBJ (Q04P36)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_802092.1
- ProteinModelPortal:   Q04P36
- SMR:   Q04P36
- STRING:   Q04P36
- GeneID:   4411773
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2937
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   ARIVEMF
- PhylomeDB:   Q04P36
- ProtClustDB:   PRK09347
- BioCyc:   LBOR355277:LBJ_2937-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 20740; Mature: 20740

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENIVNILKSIGEDPTREGLLNTPKRVKKAYDFLTSGYHVDITKVVNGAIFEESTEGMI
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHCCHHHCCCCCCEE
LVRDIEVYSLCEHHLLPFYGRAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
YAVQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCLLGAFKENMVTRSEFLDLIRT
HHHHHHHCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GST
CCC
>Mature Secondary Structure
MEENIVNILKSIGEDPTREGLLNTPKRVKKAYDFLTSGYHVDITKVVNGAIFEESTEGMI
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHCCHHHCCCCCCEE
LVRDIEVYSLCEHHLLPFYGRAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
YAVQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCLLGAFKENMVTRSEFLDLIRT
HHHHHHHCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GST
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA