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The map label for this gene is kefB [H]

Identifier: 116326938

GI number: 116326938

Start: 116870

End: 118681

Strand: Reverse

Name: kefB [H]

Synonym: LBL_0104

Alternate gene names: 116326938

Gene position: 118681-116870 (Counterclockwise)

Preceding gene: 116326939

Following gene: 116326931

Centisome position: 3.28

GC content: 38.85

Gene sequence:

>1812_bases
ATGCAAGACAACTTACTTGTGACTCTCATCGTATTTCTTTCAGCAGCAGTCATTTCGGTTCCATTTTTCAAAAAAATCGG
ATTAGGTTCCGTCGTAGGTTATCTTATCGGTGGAATTATTATCGGCCCTTGGGGAATCGGTCTGATAACTAATGTGGATA
GTATTCTTCATATTTCCGAATTTGGGGTTATTTTACTTTTATTTTTGATAGGTTTGGAGTTAAAACCCCAAAGACTTTGG
ATTTTGAAAAAACCCATTTTCGGTTTAGGCGGGCTTCAGGTAATTCTTACATTTTTTTTCTTCTTCACGATTCTTTCTTT
TTTAGACATGGAAAAATCCCAAGTAATCGTGATCAGTATTAGTATTTCATTATCTTCTACTGCTTTTGCCCTTCAGTTAT
TGGGAGAGAAAAACGAACTCAAAACTATGCACGGAAGAAGCGTTTTCGCAATTCTCCTGTTTCAAGATCTTGCCGTAATT
CCGATTATGGCGATACTTCCTTTTTTAACGGAATCAGTCGCCGATCCAGGATCACAAGGAAGTATAAAACAAATTGCAGT
TGCAACCGGAACAATTCTCACGGTTATCTTAGCAGGAAGATTTTTAGCGAGACCTTTGTTCAAACTCGTAGCCTCCAGTG
GGAATCATGAAATTTTCACAGCATTATCCTTACTGATCGTGATCGGAGTTTCGTTACTTATGGACCAGGTAGGACTATCG
ATGGCGCTTGGATCTTTTTTAGGCGGCGTGATCCTTGCAGACTCGGAATACAGGCACGAATTGGAATCAAATTTGGAACC
ATTCAAAGGCCTTTTTCTCGGGTTATTCTTTTTAGCCGTGGGAATGTCGATTAACCTTAGTGAAGTTTTAAAAGATCCGA
TTCTTGTACTTTTCTCTGCGCTCATTTTAATCCTCGTTAAAGCGACAGTGCTTTTTTTCATAGGAAAAATCTCTAACCAT
TCCAACGAAACTTCCTTAAATCTCTCTGTGACAATCGCCCAAGGCGGAGAATTCGCATTCGTAATCTTAGGAGTGGGCGT
TTCCTTGTCCATTCTTCCAAAAGAAAGAGCCGACTTAGTCATTGCGATCGTCACTCTCTCCATGGGCTTAACTCCGATCC
TTGGAATCTTCAAAGATAAAATTGTGGAATTGATTTTCAAAAAAGACCAAAATTTCAAAGAAGATACGATCGAAGAGCAG
AATCGAGTTATCATAGCGGGATTCGGACGATTCGGACAAATTATTGCAAGGATGCTTTTCGTTCACAGAATCGGTTTTAC
CGCATTAGAACACAATCCTGATCAGGTTAACGTTGCAAGAAAATTCGGCTATAAGATCTATTACGGAGACGCAAGTAAGT
TGAGTCTTTTAAGATCAGCGGGCGCCGAACAAGCCGACCTATTTATATTAGCAATTCAGGATATAGATATTTCCATAAAA
GTCGCCGAGTTAATAAAAAAACATTTCCCGAATTTAACGATTATCTCAAGAGCGCGAAATCGAGAACACGTTTTTAAATT
GATGGAACTCGGAATTCAAATCATTCGCAGAGATACCTTCGCTTCCGCACTTGAATTAGCGGGAGAAACTCTGAGAAAAT
TAGGTTTTATGGACTCGGAGGTGGAAAAGAAAATTAAAAAATTTAGAGCCCACGACGAATTAACCCTGAAAGGGCAGTTT
CAAATTCGAAACGACGAAAAAGAATTTATTAAATTTTCAAAGAACTCAATGCGTCAACTGGAGGTCGCTTTTGAAGCAGA
CCGCCAAGAAAAAGAAGGAAAATTATCAGATCAAAATTTGTCTTCTTTATAA

Upstream 100 bases:

>100_bases
ACGATGGCCGGGAGACGCTCACTCTTTTATAATTTCTTTCATGAAATTGTTTCCAGATCTCAAGAGAAAAAGTTCGGATT
TAAGTATTCGAGGATAAAGA

Downstream 100 bases:

>100_bases
AATACAATCGTCTCATTCTATAGAAAGACATTTCATCGTATTCAAATTAGTCAATAAATTATTTCGATTCGGATTTTATA
AAAACCGACGAAAATATAAC

Product: KefB related efflux transport protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MQDNLLVTLIVFLSAAVISVPFFKKIGLGSVVGYLIGGIIIGPWGIGLITNVDSILHISEFGVILLLFLIGLELKPQRLW
ILKKPIFGLGGLQVILTFFFFFTILSFLDMEKSQVIVISISISLSSTAFALQLLGEKNELKTMHGRSVFAILLFQDLAVI
PIMAILPFLTESVADPGSQGSIKQIAVATGTILTVILAGRFLARPLFKLVASSGNHEIFTALSLLIVIGVSLLMDQVGLS
MALGSFLGGVILADSEYRHELESNLEPFKGLFLGLFFLAVGMSINLSEVLKDPILVLFSALILILVKATVLFFIGKISNH
SNETSLNLSVTIAQGGEFAFVILGVGVSLSILPKERADLVIAIVTLSMGLTPILGIFKDKIVELIFKKDQNFKEDTIEEQ
NRVIIAGFGRFGQIIARMLFVHRIGFTALEHNPDQVNVARKFGYKIYYGDASKLSLLRSAGAEQADLFILAIQDIDISIK
VAELIKKHFPNLTIISRARNREHVFKLMELGIQIIRRDTFASALELAGETLRKLGFMDSEVEKKIKKFRAHDELTLKGQF
QIRNDEKEFIKFSKNSMRQLEVAFEADRQEKEGKLSDQNLSSL

Sequences:

>Translated_603_residues
MQDNLLVTLIVFLSAAVISVPFFKKIGLGSVVGYLIGGIIIGPWGIGLITNVDSILHISEFGVILLLFLIGLELKPQRLW
ILKKPIFGLGGLQVILTFFFFFTILSFLDMEKSQVIVISISISLSSTAFALQLLGEKNELKTMHGRSVFAILLFQDLAVI
PIMAILPFLTESVADPGSQGSIKQIAVATGTILTVILAGRFLARPLFKLVASSGNHEIFTALSLLIVIGVSLLMDQVGLS
MALGSFLGGVILADSEYRHELESNLEPFKGLFLGLFFLAVGMSINLSEVLKDPILVLFSALILILVKATVLFFIGKISNH
SNETSLNLSVTIAQGGEFAFVILGVGVSLSILPKERADLVIAIVTLSMGLTPILGIFKDKIVELIFKKDQNFKEDTIEEQ
NRVIIAGFGRFGQIIARMLFVHRIGFTALEHNPDQVNVARKFGYKIYYGDASKLSLLRSAGAEQADLFILAIQDIDISIK
VAELIKKHFPNLTIISRARNREHVFKLMELGIQIIRRDTFASALELAGETLRKLGFMDSEVEKKIKKFRAHDELTLKGQF
QIRNDEKEFIKFSKNSMRQLEVAFEADRQEKEGKLSDQNLSSL
>Mature_603_residues
MQDNLLVTLIVFLSAAVISVPFFKKIGLGSVVGYLIGGIIIGPWGIGLITNVDSILHISEFGVILLLFLIGLELKPQRLW
ILKKPIFGLGGLQVILTFFFFFTILSFLDMEKSQVIVISISISLSSTAFALQLLGEKNELKTMHGRSVFAILLFQDLAVI
PIMAILPFLTESVADPGSQGSIKQIAVATGTILTVILAGRFLARPLFKLVASSGNHEIFTALSLLIVIGVSLLMDQVGLS
MALGSFLGGVILADSEYRHELESNLEPFKGLFLGLFFLAVGMSINLSEVLKDPILVLFSALILILVKATVLFFIGKISNH
SNETSLNLSVTIAQGGEFAFVILGVGVSLSILPKERADLVIAIVTLSMGLTPILGIFKDKIVELIFKKDQNFKEDTIEEQ
NRVIIAGFGRFGQIIARMLFVHRIGFTALEHNPDQVNVARKFGYKIYYGDASKLSLLRSAGAEQADLFILAIQDIDISIK
VAELIKKHFPNLTIISRARNREHVFKLMELGIQIIRRDTFASALELAGETLRKLGFMDSEVEKKIKKFRAHDELTLKGQF
QIRNDEKEFIKFSKNSMRQLEVAFEADRQEKEGKLSDQNLSSL

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=380, Percent_Identity=27.1052631578947, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786232, Length=591, Percent_Identity=40.6091370558376, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1789749, Length=598, Percent_Identity=40.133779264214, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1786685, Length=528, Percent_Identity=27.4621212121212, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66587; Mature: 66587

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNLLVTLIVFLSAAVISVPFFKKIGLGSVVGYLIGGIIIGPWGIGLITNVDSILHISE
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCHHHHHHCHHHHHHHHH
FGVILLLFLIGLELKPQRLWILKKPIFGLGGLQVILTFFFFFTILSFLDMEKSQVIVISI
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SISLSSTAFALQLLGEKNELKTMHGRSVFAILLFQDLAVIPIMAILPFLTESVADPGSQG
EEEECHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SIKQIAVATGTILTVILAGRFLARPLFKLVASSGNHEIFTALSLLIVIGVSLLMDQVGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MALGSFLGGVILADSEYRHELESNLEPFKGLFLGLFFLAVGMSINLSEVLKDPILVLFSA
HHHHHHHCCEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHH
LILILVKATVLFFIGKISNHSNETSLNLSVTIAQGGEFAFVILGVGVSLSILPKERADLV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCEEEECCCCCCCEE
IAIVTLSMGLTPILGIFKDKIVELIFKKDQNFKEDTIEEQNRVIIAGFGRFGQIIARMLF
EEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHH
VHRIGFTALEHNPDQVNVARKFGYKIYYGDASKLSLLRSAGAEQADLFILAIQDIDISIK
HHHHCCHHCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCEEH
VAELIKKHFPNLTIISRARNREHVFKLMELGIQIIRRDTFASALELAGETLRKLGFMDSE
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VEKKIKKFRAHDELTLKGQFQIRNDEKEFIKFSKNSMRQLEVAFEADRQEKEGKLSDQNL
HHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSL
CCC
>Mature Secondary Structure
MQDNLLVTLIVFLSAAVISVPFFKKIGLGSVVGYLIGGIIIGPWGIGLITNVDSILHISE
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCHHHHHHCHHHHHHHHH
FGVILLLFLIGLELKPQRLWILKKPIFGLGGLQVILTFFFFFTILSFLDMEKSQVIVISI
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SISLSSTAFALQLLGEKNELKTMHGRSVFAILLFQDLAVIPIMAILPFLTESVADPGSQG
EEEECHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SIKQIAVATGTILTVILAGRFLARPLFKLVASSGNHEIFTALSLLIVIGVSLLMDQVGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MALGSFLGGVILADSEYRHELESNLEPFKGLFLGLFFLAVGMSINLSEVLKDPILVLFSA
HHHHHHHCCEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHH
LILILVKATVLFFIGKISNHSNETSLNLSVTIAQGGEFAFVILGVGVSLSILPKERADLV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCEEEECCCCCCCEE
IAIVTLSMGLTPILGIFKDKIVELIFKKDQNFKEDTIEEQNRVIIAGFGRFGQIIARMLF
EEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHH
VHRIGFTALEHNPDQVNVARKFGYKIYYGDASKLSLLRSAGAEQADLFILAIQDIDISIK
HHHHCCHHCCCCCCHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCEEH
VAELIKKHFPNLTIISRARNREHVFKLMELGIQIIRRDTFASALELAGETLRKLGFMDSE
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VEKKIKKFRAHDELTLKGQFQIRNDEKEFIKFSKNSMRQLEVAFEADRQEKEGKLSDQNL
HHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SSL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA