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The map label for this gene is prfA

Identifier: 116326889

GI number: 116326889

Start: 49523

End: 50587

Strand: Direct

Name: prfA

Synonym: LBL_0042

Alternate gene names: 116326889

Gene position: 49523-50587 (Clockwise)

Preceding gene: 116326886

Following gene: 116326890

Centisome position: 1.37

GC content: 42.25

Gene sequence:

>1065_bases
ATGATAGACAGACTTGAAAAAATACAAGAAAAATACCTTCGAATCAGCGAAGAGTTAAATTCGGCTAAAGACCCATCTTC
ACTTAAGAGCCTTTACAAGGAAAGATCCAGACTAACCCCTCTTTATCTCAAAGTGGAGGAATATCTCAAAATTTACAAAG
ATAAAAAAGATGCGGAAGAATTGATTCCGTCCGAAAAGGACGAGGAAATGCATTCTATGCTCAAAGAAGAAATTCGTCGA
GCGAGCAAGAAATTGGAGGAATTGGAAAAAGAACTCGAAATTCTACTTTTAACTCCCGATCCGAATTCAGGAAAAAATAT
CCTTGTGGAAATTAGGGCTGGAACCGGTGGAGAAGAGGCCGGTTTATTTGTGGCGGATCTTTTTAGAATGTATTCTAAAT
TTGCAGATAAACAAAAAATCAAAAGTGAAATCATCGATTCTGCCCCCACCGGAATCGGCGGATTGAAAGAAATTATTTTC
GCATTGGAGGACGAACGGGCTTATGACCTTTTCAAATTTGAAGGTGGTACACATCGGGTTCAAAGAATTCCAAGTACCGA
ATCCGGAGGAAGAATCCATACAAGCGCGGTGACTGTCGCGGTTCTTCCCGAAGCGGATGAAGAAGAAATCGAAATCAATG
AAAACGATCTTCGGATTGATGTATATCGTTCCTCCGGAGCAGGCGGTCAGCACGTAAACACAACCGACTCTGCAGTTCGA
ATCACTCACATTCCAACCGGAGTTGTGGTTGCTTGTCAGGATGAAAAATCCCAGCATAAAAACAAAGCCAAGGCACTGAG
GATTTTAAGCGCGAGAATTCTCGAGAAACAAACCGAAGATAAAAAACAGGCTTCGGACGCGATCAAGAAACAAATGATAG
GAAGCGGAGATCGCTCCGAACGAGTAAGAACCTACAACTTTCCTCAAGGAAGGTGCACTGATCACAGAATCGGATTCACA
AGTCATAACCTTTCCGCAATCATGGAAGGGGATCTGGAAGAACTGATCGGAGCTTTAACTGAAGAAGACAGAGCCCGAAA
AATTTCAGAAACACAAGTCCATTGA

Upstream 100 bases:

>100_bases
AGTCAGTAAGGATAGAAATCGTTCAGAAAAAACTTCTGCGACGGAAATATGGTTTTCCATAGAGTGCGTTGATTTATAAA
GGAAAGGTCGAGTTCATTCA

Downstream 100 bases:

>100_bases
GAGTTTTCGGTCGAATTTAGGGGTTGAAAAATCGACGATCGATCCCGGAGTAGAATCAATAGAAATGTTACAAGAAATCA
ACGAACCTCATCGAACACTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIDRLEKIQEKYLRISEELNSAKDPSSLKSLYKERSRLTPLYLKVEEYLKIYKDKKDAEELIPSEKDEEMHSMLKEEIRR
ASKKLEELEKELEILLLTPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKSEIIDSAPTGIGGLKEIIF
ALEDERAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEIEINENDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQTEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLEELIGALTEEDRARKISETQVH

Sequences:

>Translated_354_residues
MIDRLEKIQEKYLRISEELNSAKDPSSLKSLYKERSRLTPLYLKVEEYLKIYKDKKDAEELIPSEKDEEMHSMLKEEIRR
ASKKLEELEKELEILLLTPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKSEIIDSAPTGIGGLKEIIF
ALEDERAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEIEINENDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQTEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLEELIGALTEEDRARKISETQVH
>Mature_354_residues
MIDRLEKIQEKYLRISEELNSAKDPSSLKSLYKERSRLTPLYLKVEEYLKIYKDKKDAEELIPSEKDEEMHSMLKEEIRR
ASKKLEELEKELEILLLTPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKSEIIDSAPTGIGGLKEIIF
ALEDERAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEIEINENDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQTEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLEELIGALTEEDRARKISETQVH

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=45.2054794520548, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI34577120, Length=344, Percent_Identity=36.3372093023256, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=44.1025641025641, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=47.3087818696884, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=35.9882005899705, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=277, Percent_Identity=35.7400722021661, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=41.6167664670659, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI19921226, Length=296, Percent_Identity=40.8783783783784, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_LEPBJ (Q04W81)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799597.1
- ProteinModelPortal:   Q04W81
- SMR:   Q04W81
- STRING:   Q04W81
- GeneID:   4410670
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0093
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q04W81
- ProtClustDB:   PRK00591
- BioCyc:   LBOR355277:LBJ_0093-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39998; Mature: 39998

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRLEKIQEKYLRISEELNSAKDPSSLKSLYKERSRLTPLYLKVEEYLKIYKDKKDAEE
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCHHHH
LIPSEKDEEMHSMLKEEIRRASKKLEELEKELEILLLTPDPNSGKNILVEIRAGTGGEEA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFVADLFRMYSKFADKQKIKSEIIDSAPTGIGGLKEIIFALEDERAYDLFKFEGGTHRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHH
QRIPSTESGGRIHTSAVTVAVLPEADEEEIEINENDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQTEDKKQASDAIKKQMIGSGDRSE
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRTYNFPQGRCTDHRIGFTSHNLSAIMEGDLEELIGALTEEDRARKISETQVH
CCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIDRLEKIQEKYLRISEELNSAKDPSSLKSLYKERSRLTPLYLKVEEYLKIYKDKKDAEE
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCHHHH
LIPSEKDEEMHSMLKEEIRRASKKLEELEKELEILLLTPDPNSGKNILVEIRAGTGGEEA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFVADLFRMYSKFADKQKIKSEIIDSAPTGIGGLKEIIFALEDERAYDLFKFEGGTHRV
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHH
QRIPSTESGGRIHTSAVTVAVLPEADEEEIEINENDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQTEDKKQASDAIKKQMIGSGDRSE
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRTYNFPQGRCTDHRIGFTSHNLSAIMEGDLEELIGALTEEDRARKISETQVH
CCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA