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The map label for this gene is hisH [H]

Identifier: 116326881

GI number: 116326881

Start: 41363

End: 41995

Strand: Reverse

Name: hisH [H]

Synonym: LBL_0033

Alternate gene names: 116326881

Gene position: 41995-41363 (Counterclockwise)

Preceding gene: 116326882

Following gene: 116326880

Centisome position: 1.16

GC content: 36.97

Gene sequence:

>633_bases
ATGATAGCTATTCTAGATTACGGAATGGGAAACATTCATTCCTGTATCAAAGCTGTATCTCTTTATACAAAAGATTTCGT
TTATACGAACAATAAAACAACGATCGAAAATTCAAAAGCTCTAATATTGCCCGGTGACGGGCATTTTGATCGGGCTATGG
AGAATTTGAATTCGACCGGACTTCGAGAAACGATCGATAAACACGTAAATTCCGGAAAACCTCTTTTCGGGATTTGTATA
GGTTTTCAAATTCTTTTCGAGTCTTCAGAAGAAACTACTCAAGGTACTAAAAAAGAGCAGATTGAAGGTTTGGGATATAT
CAAAGGAAAGGTCAAAAAATTTCAGGGAAAAGATTTTAAAGTTCCTCATATCGGTTGGAATCGACTTCAAATTCGTAGAA
AAGATAAAAGTATTCTTTTAAAGGGAATCGGCGATCAGTCTTTTTTTTATTTCATCCATTCTTACAGACCGACGAGCGCA
GAGGGAAATGCGATTACTGGACTTTGCGATTATTACCAAGAGAAATTTCCGGCCGTTGTGGAGAAGGATAATATTTTCGG
AACTCAGTTCCACCCCGAAAAGTCTCATACTCACGGACTTAAACTATTGGAGAATTTCATTCATTTCGTATGA

Upstream 100 bases:

>100_bases
ACGAATCTATTTTTAAGGCTCTAGGTAAAGCTCTTAGAATGGCGATCGCACAAGACTCGGCGACTGCAGGCGCGATTCCT
TCCACAAAAGGAGTTTTGGA

Downstream 100 bases:

>100_bases
TTATCATTCCAGCCATCGATCTATTCGATAATTGTGCAGTTCGTTTATTCAAAGGAAATTACAAGGAAAAGAAAATTTAT
TCTTCCGAACCTTGGAAACT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIAILDYGMGNIHSCIKAVSLYTKDFVYTNNKTTIENSKALILPGDGHFDRAMENLNSTGLRETIDKHVNSGKPLFGICI
GFQILFESSEETTQGTKKEQIEGLGYIKGKVKKFQGKDFKVPHIGWNRLQIRRKDKSILLKGIGDQSFFYFIHSYRPTSA
EGNAITGLCDYYQEKFPAVVEKDNIFGTQFHPEKSHTHGLKLLENFIHFV

Sequences:

>Translated_210_residues
MIAILDYGMGNIHSCIKAVSLYTKDFVYTNNKTTIENSKALILPGDGHFDRAMENLNSTGLRETIDKHVNSGKPLFGICI
GFQILFESSEETTQGTKKEQIEGLGYIKGKVKKFQGKDFKVPHIGWNRLQIRRKDKSILLKGIGDQSFFYFIHSYRPTSA
EGNAITGLCDYYQEKFPAVVEKDNIFGTQFHPEKSHTHGLKLLENFIHFV
>Mature_210_residues
MIAILDYGMGNIHSCIKAVSLYTKDFVYTNNKTTIENSKALILPGDGHFDRAMENLNSTGLRETIDKHVNSGKPLFGICI
GFQILFESSEETTQGTKKEQIEGLGYIKGKVKKFQGKDFKVPHIGWNRLQIRRKDKSILLKGIGDQSFFYFIHSYRPTSA
EGNAITGLCDYYQEKFPAVVEKDNIFGTQFHPEKSHTHGLKLLENFIHFV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=34.1346153846154, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=33.9449541284404, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23841; Mature: 23841

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAILDYGMGNIHSCIKAVSLYTKDFVYTNNKTTIENSKALILPGDGHFDRAMENLNSTG
CEEEEECCCCHHHHHHHHHHHHHHHEEEECCCEEECCCCEEEECCCCCHHHHHHHCCCCC
LRETIDKHVNSGKPLFGICIGFQILFESSEETTQGTKKEQIEGLGYIKGKVKKFQGKDFK
HHHHHHHHHCCCCCHHHHHHHHHHEECCCCHHHCCCHHHHHCCCCHHHHHHHHCCCCCCC
VPHIGWNRLQIRRKDKSILLKGIGDQSFFYFIHSYRPTSAEGNAITGLCDYYQEKFPAVV
CCCCCCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEE
EKDNIFGTQFHPEKSHTHGLKLLENFIHFV
ECCCEECCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAILDYGMGNIHSCIKAVSLYTKDFVYTNNKTTIENSKALILPGDGHFDRAMENLNSTG
CEEEEECCCCHHHHHHHHHHHHHHHEEEECCCEEECCCCEEEECCCCCHHHHHHHCCCCC
LRETIDKHVNSGKPLFGICIGFQILFESSEETTQGTKKEQIEGLGYIKGKVKKFQGKDFK
HHHHHHHHHCCCCCHHHHHHHHHHEECCCCHHHCCCHHHHHCCCCHHHHHHHHCCCCCCC
VPHIGWNRLQIRRKDKSILLKGIGDQSFFYFIHSYRPTSAEGNAITGLCDYYQEKFPAVV
CCCCCCCEEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEE
EKDNIFGTQFHPEKSHTHGLKLLENFIHFV
ECCCEECCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]