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The map label for this gene is gyrB [H]

Identifier: 116326860

GI number: 116326860

Start: 9424

End: 11349

Strand: Direct

Name: gyrB [H]

Synonym: LBL_0008

Alternate gene names: 116326860

Gene position: 9424-11349 (Clockwise)

Preceding gene: 116326859

Following gene: 116326861

Centisome position: 0.26

GC content: 39.77

Gene sequence:

>1926_bases
ATGAGCCAAAAAGAAACAAGCTACAGTGCTGGTCAGATCAAGATTTTAGAGGGTCTGGAAGCTGTTAGAAAACGTCCTGG
GATGTATATCGGAACCCAGGATGAGACTGGACTTCACAAAATGGTCTACGAGGTGGTTGATAACTCTGTAGATGAAGCAA
TGGCAGGTCATTGTACCGAAATTAGAATTAGTATTTTGCCGAATAATATCATCGAAGTTAAAGACAACGGTCGAGGGATT
CCGGTCGATATTCATCCCGATAAAAAAATTTCCACAATAGAAGTCGTAATGACCATTCTTCATGCGGGTGGTAAGTTCGA
AAACGACGCTTATAAAGTTTCGGGAGGTCTCCATGGTGTAGGGGTTTCCGTAGTAAACGCACTTTCTGAATATCTTGAAG
TCGAAGTTCATCAGAAAGGAAAAATTTATACTCAGAAATACGAGAAAGGTATTCCGGTTTCTCCAGTGGAAATTAAGGGA
GATTCTTCGGAAAGAGGAACCATCGTTCGATTCAAACCGGATGCTACTATTTTTACCACAGTAGACTTTCAATTCGATAT
TCTTTCCGCTCGATTTAGGGAATTAGCTTTTTTGAATAAAGGTCTTGTGCTGATCGTTGAGGACCGCAGACGAGGGGCGG
AAGGAGAAAATCTTCTTCGAAACGAATTTCAATTTTCCGGGGGAATCGTTTCTTTCGTCGAGCATATTAATGAAAATAAA
CATCCTATGCATAAGGTAATTCACTTTGAACGGAATAAAGACGACGTTTTGGCGGAAATTTCGATTCAATATTCGGAAAC
GTATACAGAGAACATTTTTTGTTTTACGAACAACATCAATAACAACTTAGGCGGGACCCATTTGGAAGGATTTCGCGCGG
CTCTTACAAGAACCTTAAACGACTTTCTGAAAAAAGATACGACTCTTTCTAAAAAACATCCGACCGGTTTGTCCGGAGAA
GATGTAAAAGAAGGATTAACCGCGGTCATCTCGATCAAAATTCCTCAACCTCAGTTCAACTCTCAAACAAAAGAAAAACT
AGTAAACGCGGAAATTAAAGGGATTATGCAGACTTTAAGTTCGGAAGGTTTGACTTTATTCTTTGAGGAAAATCCGAATA
TCACTAAAAAGATATTAGAAAAATGTATTCTTTCCGCAAAAGCAAGAGAAGCGGCCCGTAAGGCGAGAGATTTAACACGA
AGAAAAACGGTTCTTGAAGGAGGAGGGCTTCCGGGGAAGCTCGCTGATTGTTCTGAAAAAGATCCTGCGTTTTCCGAACT
TTATCTCGTTGAGGGAGATTCCGCAGGTGGTTCCGCAAAACAAGGAAGAGATAGAAATACACAGGCGATTCTTCCTCTGA
AAGGAAAAATTTTAAACGTTGAAAAGGCGAGGTTGGATAAAATTCTTTCCAGCGAAGAAATTCGAATTTTGGTTTCCGCC
CTTGGAACCGGGATCGGCGAAGACGAGTTTAATATCAATAAAATCCGATATCATAAAATTATGATAATGACGGATGCGGA
TATCGACGGTTCACACATTCGTACATTACTTCTTACATTCTTTTTTCGTTATATGCGTCCTGTAATCGAAAGAGGTTATC
TCTATGTGGCGCAACCGCCTTTGTATCTTATTAAACATGGTAAAAATTCAACTTATGCTTATTCGGATAAGGAAAAAGAG
GAATTATTGAAGAACGTAGGGACTGAAAAAGTTGTGATTCAAAGATACAAAGGTCTAGGTGAAATGAATCCGGAACAACT
TTGGGAAACCACTATGGATCCTTCGAACCGAGTCGTTTTGAAAGTGAAATTAGACGATTTCGTGGAAGCGGAAGAAACGT
TTAACATCCTTATGGGTGATGAAGTTCAACCTAGAAAACAATTCATCGAAATTAATGCCGCCAAGGTTGCAAACTTGGAT
CTTTGA

Upstream 100 bases:

>100_bases
AAAAACGTTATTTAGAGATTCTTCAATATCTTTGATAAGCTGATGAATTTCCTTGCCTCCCTACGGTTTTCAGGAAAACT
ATTCCTGAGGTCCGAAAAAA

Downstream 100 bases:

>100_bases
TCCTAGGGGAATTCTAAAATGAGTCAAGAGAATGAAACAAAAGTCCTAAACTATAATATCGCCGGAAAGCCCGATATTGC
GGATGCTCTTAAAAACGGCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MSQKETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIRISILPNNIIEVKDNGRGI
PVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKIYTQKYEKGIPVSPVEIKG
DSSERGTIVRFKPDATIFTTVDFQFDILSARFRELAFLNKGLVLIVEDRRRGAEGENLLRNEFQFSGGIVSFVEHINENK
HPMHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDTTLSKKHPTGLSGE
DVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTR
RKTVLEGGGLPGKLADCSEKDPAFSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRILVSA
LGTGIGEDEFNINKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYAYSDKEKE
ELLKNVGTEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNILMGDEVQPRKQFIEINAAKVANLD
L

Sequences:

>Translated_641_residues
MSQKETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIRISILPNNIIEVKDNGRGI
PVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKIYTQKYEKGIPVSPVEIKG
DSSERGTIVRFKPDATIFTTVDFQFDILSARFRELAFLNKGLVLIVEDRRRGAEGENLLRNEFQFSGGIVSFVEHINENK
HPMHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDTTLSKKHPTGLSGE
DVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTR
RKTVLEGGGLPGKLADCSEKDPAFSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRILVSA
LGTGIGEDEFNINKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYAYSDKEKE
ELLKNVGTEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNILMGDEVQPRKQFIEINAAKVANLD
L
>Mature_640_residues
SQKETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIRISILPNNIIEVKDNGRGIP
VDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKIYTQKYEKGIPVSPVEIKGD
SSERGTIVRFKPDATIFTTVDFQFDILSARFRELAFLNKGLVLIVEDRRRGAEGENLLRNEFQFSGGIVSFVEHINENKH
PMHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDTTLSKKHPTGLSGED
VKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRR
KTVLEGGGLPGKLADCSEKDPAFSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRILVSAL
GTGIGEDEFNINKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYAYSDKEKEE
LLKNVGTEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNILMGDEVQPRKQFIEINAAKVANLDL

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=611, Percent_Identity=28.1505728314239, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI19913406, Length=606, Percent_Identity=26.2376237623762, Blast_Score=146, Evalue=7e-35,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=56.2724014336918, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=609, Percent_Identity=38.9162561576355, Blast_Score=411, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17535065, Length=635, Percent_Identity=24.8818897637795, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=533, Percent_Identity=26.078799249531, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=188, Percent_Identity=31.3829787234043, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=646, Percent_Identity=27.0897832817337, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17136538, Length=654, Percent_Identity=26.2996941896024, Blast_Score=149, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71884; Mature: 71753

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTE
CCCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEE
IRISILPNNIIEVKDNGRGIPVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGV
EEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEYLEVEVHQKGKIYTQKYEKGIPVSPVEIKGDSSERGTIVRFKPDATIFTT
HHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCEEEEE
VDFQFDILSARFRELAFLNKGLVLIVEDRRRGAEGENLLRNEFQFSGGIVSFVEHINENK
EEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCC
HPMHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLN
CCHHHHHEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DFLKKDTTLSKKHPTGLSGEDVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLS
HHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
SEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRKTVLEGGGLPGKLADCSEK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DPAFSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRILVSA
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCCHHHHHHHH
LGTGIGEDEFNINKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPP
HCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYLIKHGKNSTYAYSDKEKEELLKNVGTEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVL
EEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEE
KVKLDDFVEAEETFNILMGDEVQPRKQFIEINAAKVANLDL
EEECCHHHCHHHHHEEEECCCCCCCHHHEEECCHHEECCCC
>Mature Secondary Structure 
SQKETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTE
CCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEE
IRISILPNNIIEVKDNGRGIPVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGV
EEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEYLEVEVHQKGKIYTQKYEKGIPVSPVEIKGDSSERGTIVRFKPDATIFTT
HHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCEEEEE
VDFQFDILSARFRELAFLNKGLVLIVEDRRRGAEGENLLRNEFQFSGGIVSFVEHINENK
EEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCC
HPMHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLN
CCHHHHHEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DFLKKDTTLSKKHPTGLSGEDVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLS
HHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
SEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRKTVLEGGGLPGKLADCSEK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DPAFSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRILVSA
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCCHHHHHHHH
LGTGIGEDEFNINKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPP
HCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCC
LYLIKHGKNSTYAYSDKEKEELLKNVGTEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVL
EEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEE
KVKLDDFVEAEETFNILMGDEVQPRKQFIEINAAKVANLDL
EEECCHHHCHHHHHEEEECCCCCCCHHHEEECCHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]