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The map label for this gene is ilvE [H]

Identifier: 116326854

GI number: 116326854

Start: 2049

End: 2972

Strand: Direct

Name: ilvE [H]

Synonym: LBL_0002

Alternate gene names: 116326854

Gene position: 2049-2972 (Clockwise)

Preceding gene: NA

Following gene: 116326855

Centisome position: 0.06

GC content: 39.61

Gene sequence:

>924_bases
ATGGCAGAATCCATCAAGAAACTATCCTATTTTGAAGGAAAAATCCTTCCAGAATCAGAAGCGAAGATCAGCATTCAAAC
TCACGCACTTCAGTACGGAACCACCGTCTTCGGAGGACTCCGCGGTTATTACGACAAGGATACGGATAATATCTATCTCT
TTCGTATCTTAGATCATTATCAAAGACTGATAAACTCGACTCGAATCATGCAATTGAAACTCGACAAAACAAAAGAAGAA
TTGAGAGACATAACTATTGATTTGATTCGTCAGTGCGGTTACAAAGAAAACATTTATCTTCGTCCTTTCGTTTATACGTC
GGCGTTGCAACTTTCACCTCGTTTTCACGACGTTCCCACCGAACTCGCAATTTATATTCTTCAGTTGAACGATTACCTGG
ATACCAAGCATGGATTAAAGACTATGGTTTCCAGTTGGAGAAGATTCGATGATGCAGTAATACCGACCTTGTCGAAAGTT
TCCGGCGGTTACGTGAACTCGGCGCTTGCAAAATCGGAAGCGGTTCAAAACGGATTTGATGAAGCGATCTTTTTGGATTC
GAGAGGATTTGTAAGCGAAGGTTCCGCGGAAAACATTTTCTTAGTTCGCGATGGTAAGATCATTACGCCAGGCATTAATT
CTTCTCTGCTCGAAGGAATTACAAGAAGGAGTGTTCTCCAAATTGCAAAAGATAATGGAATCGAAGTAATCGAAAGGGAT
ATTTCCAGAAGTGAACTTTACATATCGGACGAGGTTTTCTTTTCGGGAACAGGAGTACAAATCGCATGGGTTTCTGAAAT
CGATCATAGAAAGATTGGCAATTCAAAGATGGGACCAATTACAAAAAAGATTCAAAGTTTATTCTTCAATCTTGTAATTA
ACAAAGAAGAAAAATACAGACATTGGTTGACTCCGGTTTATTGA

Upstream 100 bases:

>100_bases
GAGATGTTTCGCAAGTATTGGCCTTTGATCGTAAGATGAATCGACAACAGACCTGATATATTGATTCATGGAATTATATC
ACTTATCAGGTTCCTGTTCT

Downstream 100 bases:

>100_bases
AAAAGAATGTTTCGCTCTGCGTTTCAATTCGACGATATTTTAGGACAAGAGGTCGCTCTTACTTTTTTAAAAAGGTATAC
GTCAAAACCGGAGACAATTC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAESIKKLSYFEGKILPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRIMQLKLDKTKEE
LRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTELAIYILQLNDYLDTKHGLKTMVSSWRRFDDAVIPTLSKV
SGGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIAKDNGIEVIERD
ISRSELYISDEVFFSGTGVQIAWVSEIDHRKIGNSKMGPITKKIQSLFFNLVINKEEKYRHWLTPVY

Sequences:

>Translated_307_residues
MAESIKKLSYFEGKILPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRIMQLKLDKTKEE
LRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTELAIYILQLNDYLDTKHGLKTMVSSWRRFDDAVIPTLSKV
SGGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIAKDNGIEVIERD
ISRSELYISDEVFFSGTGVQIAWVSEIDHRKIGNSKMGPITKKIQSLFFNLVINKEEKYRHWLTPVY
>Mature_306_residues
AESIKKLSYFEGKILPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHYQRLINSTRIMQLKLDKTKEEL
RDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPTELAIYILQLNDYLDTKHGLKTMVSSWRRFDDAVIPTLSKVS
GGYVNSALAKSEAVQNGFDEAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIAKDNGIEVIERDI
SRSELYISDEVFFSGTGVQIAWVSEIDHRKIGNSKMGPITKKIQSLFFNLVINKEEKYRHWLTPVY

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=302, Percent_Identity=37.7483443708609, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 35113; Mature: 34981

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESIKKLSYFEGKILPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHY
CCCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCHHHHHHCCCCCCCCCCEEEEHHHHHH
QRLINSTRIMQLKLDKTKEELRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPT
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHEECCCCCCCCCH
ELAIYILQLNDYLDTKHGLKTMVSSWRRFDDAVIPTLSKVSGGYVNSALAKSEAVQNGFD
HHEEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIAKDNGIEVIERD
CEEEEECCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
ISRSELYISDEVFFSGTGVQIAWVSEIDHRKIGNSKMGPITKKIQSLFFNLVINKEEKYR
CCCCEEEEECEEEEECCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
HWLTPVY
HCCCCCC
>Mature Secondary Structure 
AESIKKLSYFEGKILPESEAKISIQTHALQYGTTVFGGLRGYYDKDTDNIYLFRILDHY
CCHHHHHHHHCCCCCCCCCCEEEEEEHHHHHCCHHHHHHCCCCCCCCCCEEEEHHHHHH
QRLINSTRIMQLKLDKTKEELRDITIDLIRQCGYKENIYLRPFVYTSALQLSPRFHDVPT
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHEECCCCCCCCCH
ELAIYILQLNDYLDTKHGLKTMVSSWRRFDDAVIPTLSKVSGGYVNSALAKSEAVQNGFD
HHEEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
EAIFLDSRGFVSEGSAENIFLVRDGKIITPGINSSLLEGITRRSVLQIAKDNGIEVIERD
CEEEEECCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
ISRSELYISDEVFFSGTGVQIAWVSEIDHRKIGNSKMGPITKKIQSLFFNLVINKEEKYR
CCCCEEEEECEEEEECCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
HWLTPVY
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]