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The map label for this gene is gidA

Identifier: 116326853

GI number: 116326853

Start: 1

End: 1908

Strand: Reverse

Name: gidA

Synonym: LBL_0001

Alternate gene names: 116326853

Gene position: 1908-1 (Counterclockwise)

Preceding gene: 116326863

Following gene: NA

Centisome position: 0.05

GC content: 42.71

Gene sequence:

>1908_bases
ATGATCGAATCCAAAAACCAATCTTTTTTTCCGAATCGTTTTGATTGTGTCGTAGTAGGTGCGGGTCATGCAGGTTCGGA
AGCGGCTTACATCGCTTCAAAAGGAGGAGCAAAAACTCTTTTGATCACGATGAACTTGGACACGATCGGTCAGATGTCTT
GCAACCCTGCGATCGGCGGCATCGCCAAAGGGCATATGGTGAGGGAGGTAGACGCACTCGGCGGTATCATGGGCAAGGTG
ATTGATAATACCGGAATTCAATTTAAGATGCTCAACACTTCCAAAGGTCCAAGCGTTTGGGCGCCACGCGCTCAAGCGGA
AAAAAAAGAATATCAACTGAAAGTAAAACATACTCTTGAAGCGGAAAAGAATCTTTCAATGCGACAGGACACGGTTGAAG
AGCTTCTCATTGAAAACGATCAAGTGATTGGCGTGAAGACCGGTCGTGGTTTTGAGATTTATACGAACCATGTCATTTTA
ACCACTGGAACATTCTTATCTTCTCTCGTTCACATCGGAACATATCAGAATGAAAACGGAAGAATGTGCGAGCCAACGGT
CAAAGGTCTTTCTAAGTCCTTGGCAAAATACAATTTAAAGTTAGGAAGACTGAAGACAGGAACTCCGCCTCGGATTCATA
AGAACTCGGTCGACTTGAGTGTGCTTGCGATTCAGGACGGTGATGCGAATCCTTCTCCATTTTCTTTTTCAACGGAGAAG
ATCACTCGCAGACAAATTCCTTGTTACATCACTTATACAAATGTTGAAACTCACAAACTCATTCACGAAAATCTAAGTTT
ATCTCCGATGTATTCGGGACAAATTCAAAGTACCGGACCTCGTTATTGTCCTTCGATCGAAGACAAGGTGGTTCGATTTG
CGGATCGCGAACGACATCAAGTTTTTCTCGAGCCGGAAGGATATGAAACGACCGAGATCTATCTCAACGGAGTTTCCACA
AGTCTTCCGGAAGAAGTTCAATGGAAGTTGGTGCGTTCTCTCAAGGGTTTAGAAAATGCGGAGATTGTGAGACCCGGTTA
TGCGATCGAATATGACTATGTTGATCCAACCGAACTCAAACCTACTCTCGAAACGAAGAAGATCAAAGGTCTTTATCACG
CGGGGCAAATCAACGGAACTACCGGCTATGAAGAAGCGGCCGCGCAAGGTTTGGTTGCGGCTTATAGCGTTCTTCATTCT
CTGAAGAATTTAGATCCGTTGTTGTTTAAGAGAAGCGAATCGTACATCGGAGTTTTGATCGACGATTTAGTTTATAAAGG
TGTTGAAGATCCATATAGAATGTTTACTTCTCGCGCAGAACACAGACTTCTTCTCAGACAAGACAACGCCGATCAAAGAC
TGATGAAATACGGATACGAACTTGGTCTCGTCGATCAAGAAAGTTACGATCGTATGAAAGATAAATACGAAAGAGTGAAC
TCGGTTCGAGAAAAGATTTATCAGATCCCGCTCAAACCTTCGGACGAATTTCAGAATCTTTTGGATCAAAAAGGGATCAC
GAACTATAAGTTTGGTATGAAGTTGGATTCGTTTTTGAAGCGTCCCGAAATTAAAATCGAAGACGTAGAGTTCATGCTTC
CTGAAGTCAGTTCTTGGTCGGAGTCGGAGAAAAATATTCTTGAAATGGAAATCAAATATGAAGGTTACATCAAACGAGAG
CTCGAAACGATTCAATGGAAGAATAAATATTTGGATCTTGCGATTCCGGAAGATATAAACTATGAATCGATTGCCGGACT
CAAGAAAGAGGCGATTCAAAAATTAAAAACTCATAAGCCGATGACTTTGGAAAAGGCCGGACAGATTTCCGGTGTGGATC
CGAGCGACGTCGATTTGCTGTTGTATCACATTAAAGGAAAAAGAAAACAAGAAGCGGAAGCATCTTGA

Upstream 100 bases:

>100_bases
TATATCAGGTCTGTTGTCGATTCATCTTACGATCAAAGGCCAATACTTGCGAAACATCTCATCTTAAATCAGTAATTTAT
TTTTAGGAAGTTGAATCTCG

Downstream 100 bases:

>100_bases
GCATGGTCTGTTTTTCACGAGGTGAATTGTAGGAACTCCTACTTCGGTTGGAGATTTTTCCTGAAAATTGCGGTCGGAAT
TCTGACAAGGGTTTTGGTTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIESKNQSFFPNRFDCVVVGAGHAGSEAAYIASKGGAKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGIMGKV
IDNTGIQFKMLNTSKGPSVWAPRAQAEKKEYQLKVKHTLEAEKNLSMRQDTVEELLIENDQVIGVKTGRGFEIYTNHVIL
TTGTFLSSLVHIGTYQNENGRMCEPTVKGLSKSLAKYNLKLGRLKTGTPPRIHKNSVDLSVLAIQDGDANPSPFSFSTEK
ITRRQIPCYITYTNVETHKLIHENLSLSPMYSGQIQSTGPRYCPSIEDKVVRFADRERHQVFLEPEGYETTEIYLNGVST
SLPEEVQWKLVRSLKGLENAEIVRPGYAIEYDYVDPTELKPTLETKKIKGLYHAGQINGTTGYEEAAAQGLVAAYSVLHS
LKNLDPLLFKRSESYIGVLIDDLVYKGVEDPYRMFTSRAEHRLLLRQDNADQRLMKYGYELGLVDQESYDRMKDKYERVN
SVREKIYQIPLKPSDEFQNLLDQKGITNYKFGMKLDSFLKRPEIKIEDVEFMLPEVSSWSESEKNILEMEIKYEGYIKRE
LETIQWKNKYLDLAIPEDINYESIAGLKKEAIQKLKTHKPMTLEKAGQISGVDPSDVDLLLYHIKGKRKQEAEAS

Sequences:

>Translated_635_residues
MIESKNQSFFPNRFDCVVVGAGHAGSEAAYIASKGGAKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGIMGKV
IDNTGIQFKMLNTSKGPSVWAPRAQAEKKEYQLKVKHTLEAEKNLSMRQDTVEELLIENDQVIGVKTGRGFEIYTNHVIL
TTGTFLSSLVHIGTYQNENGRMCEPTVKGLSKSLAKYNLKLGRLKTGTPPRIHKNSVDLSVLAIQDGDANPSPFSFSTEK
ITRRQIPCYITYTNVETHKLIHENLSLSPMYSGQIQSTGPRYCPSIEDKVVRFADRERHQVFLEPEGYETTEIYLNGVST
SLPEEVQWKLVRSLKGLENAEIVRPGYAIEYDYVDPTELKPTLETKKIKGLYHAGQINGTTGYEEAAAQGLVAAYSVLHS
LKNLDPLLFKRSESYIGVLIDDLVYKGVEDPYRMFTSRAEHRLLLRQDNADQRLMKYGYELGLVDQESYDRMKDKYERVN
SVREKIYQIPLKPSDEFQNLLDQKGITNYKFGMKLDSFLKRPEIKIEDVEFMLPEVSSWSESEKNILEMEIKYEGYIKRE
LETIQWKNKYLDLAIPEDINYESIAGLKKEAIQKLKTHKPMTLEKAGQISGVDPSDVDLLLYHIKGKRKQEAEAS
>Mature_635_residues
MIESKNQSFFPNRFDCVVVGAGHAGSEAAYIASKGGAKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGIMGKV
IDNTGIQFKMLNTSKGPSVWAPRAQAEKKEYQLKVKHTLEAEKNLSMRQDTVEELLIENDQVIGVKTGRGFEIYTNHVIL
TTGTFLSSLVHIGTYQNENGRMCEPTVKGLSKSLAKYNLKLGRLKTGTPPRIHKNSVDLSVLAIQDGDANPSPFSFSTEK
ITRRQIPCYITYTNVETHKLIHENLSLSPMYSGQIQSTGPRYCPSIEDKVVRFADRERHQVFLEPEGYETTEIYLNGVST
SLPEEVQWKLVRSLKGLENAEIVRPGYAIEYDYVDPTELKPTLETKKIKGLYHAGQINGTTGYEEAAAQGLVAAYSVLHS
LKNLDPLLFKRSESYIGVLIDDLVYKGVEDPYRMFTSRAEHRLLLRQDNADQRLMKYGYELGLVDQESYDRMKDKYERVN
SVREKIYQIPLKPSDEFQNLLDQKGITNYKFGMKLDSFLKRPEIKIEDVEFMLPEVSSWSESEKNILEMEIKYEGYIKRE
LETIQWKNKYLDLAIPEDINYESIAGLKKEAIQKLKTHKPMTLEKAGQISGVDPSDVDLLLYHIKGKRKQEAEAS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=644, Percent_Identity=40.9937888198758, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI19882217, Length=669, Percent_Identity=39.4618834080717, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI183227703, Length=684, Percent_Identity=38.8888888888889, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=49.8387096774194, Blast_Score=621, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17534255, Length=625, Percent_Identity=38.4, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321202, Length=635, Percent_Identity=42.3622047244094, Blast_Score=468, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=41.9968304278922, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_LEPBJ (Q04WG0)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799518.1
- ProteinModelPortal:   Q04WG0
- SMR:   Q04WG0
- STRING:   Q04WG0
- GeneID:   4412080
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0001
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q04WG0
- ProtClustDB:   PRK05192
- BioCyc:   LBOR355277:LBJ_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 71796; Mature: 71796

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESKNQSFFPNRFDCVVVGAGHAGSEAAYIASKGGAKTLLITMNLDTIGQMSCNPAIGG
CCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCC
IAKGHMVREVDALGGIMGKVIDNTGIQFKMLNTSKGPSVWAPRAQAEKKEYQLKVKHTLE
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCHHEEEEEEEHHH
AEKNLSMRQDTVEELLIENDQVIGVKTGRGFEIYTNHVILTTGTFLSSLVHIGTYQNENG
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECEEEEEECHHHHHHHHHCCEECCCC
RMCEPTVKGLSKSLAKYNLKLGRLKTGTPPRIHKNSVDLSVLAIQDGDANPSPFSFSTEK
CCCCHHHHHHHHHHHHHCCEECEECCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCHHH
ITRRQIPCYITYTNVETHKLIHENLSLSPMYSGQIQSTGPRYCPSIEDKVVRFADRERHQ
HHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
VFLEPEGYETTEIYLNGVSTSLPEEVQWKLVRSLKGLENAEIVRPGYAIEYDYVDPTELK
EEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC
PTLETKKIKGLYHAGQINGTTGYEEAAAQGLVAAYSVLHSLKNLDPLLFKRSESYIGVLI
CCHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCHHHHHH
DDLVYKGVEDPYRMFTSRAEHRLLLRQDNADQRLMKYGYELGLVDQESYDRMKDKYERVN
HHHHHCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SVREKIYQIPLKPSDEFQNLLDQKGITNYKFGMKLDSFLKRPEIKIEDVEFMLPEVSSWS
HHHHHHHCCCCCCCHHHHHHHHHCCCCCEECCCCHHHHHCCCCCEEECHHEECCCCCCCC
ESEKNILEMEIKYEGYIKRELETIQWKNKYLDLAIPEDINYESIAGLKKEAIQKLKTHKP
CCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
MTLEKAGQISGVDPSDVDLLLYHIKGKRKQEAEAS
CCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIESKNQSFFPNRFDCVVVGAGHAGSEAAYIASKGGAKTLLITMNLDTIGQMSCNPAIGG
CCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCC
IAKGHMVREVDALGGIMGKVIDNTGIQFKMLNTSKGPSVWAPRAQAEKKEYQLKVKHTLE
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHCCCHHEEEEEEEHHH
AEKNLSMRQDTVEELLIENDQVIGVKTGRGFEIYTNHVILTTGTFLSSLVHIGTYQNENG
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECEEEEEECHHHHHHHHHCCEECCCC
RMCEPTVKGLSKSLAKYNLKLGRLKTGTPPRIHKNSVDLSVLAIQDGDANPSPFSFSTEK
CCCCHHHHHHHHHHHHHCCEECEECCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCHHH
ITRRQIPCYITYTNVETHKLIHENLSLSPMYSGQIQSTGPRYCPSIEDKVVRFADRERHQ
HHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
VFLEPEGYETTEIYLNGVSTSLPEEVQWKLVRSLKGLENAEIVRPGYAIEYDYVDPTELK
EEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC
PTLETKKIKGLYHAGQINGTTGYEEAAAQGLVAAYSVLHSLKNLDPLLFKRSESYIGVLI
CCHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCHHHHHH
DDLVYKGVEDPYRMFTSRAEHRLLLRQDNADQRLMKYGYELGLVDQESYDRMKDKYERVN
HHHHHCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
SVREKIYQIPLKPSDEFQNLLDQKGITNYKFGMKLDSFLKRPEIKIEDVEFMLPEVSSWS
HHHHHHHCCCCCCCHHHHHHHHHCCCCCEECCCCHHHHHCCCCCEEECHHEECCCCCCCC
ESEKNILEMEIKYEGYIKRELETIQWKNKYLDLAIPEDINYESIAGLKKEAIQKLKTHKP
CCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
MTLEKAGQISGVDPSDVDLLLYHIKGKRKQEAEAS
CCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA