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The map label for this gene is alsB [H]

Identifier: 116249418

GI number: 116249418

Start: 822396

End: 823424

Strand: Direct

Name: alsB [H]

Synonym: pRL120756

Alternate gene names: 116249418

Gene position: 822396-823424 (Clockwise)

Preceding gene: 116249417

Following gene: 116249419

Centisome position: 94.53

GC content: 58.7

Gene sequence:

>1029_bases
TTGCGCAAATTTCTAAGCACGACCGCATTGGCGGTCCTTACATCGACGCTTTTCGCCGGCGCGGCCAGCGCGGACGCGGA
AAATCCGTTCCGCTGCAAGCCCGGTGAAAAATACGTCATGAACGTCATGGTATCGGGCGTCGAATATTGGTTCCCGGTCT
ATGAAATGTTCAAGCAGGCCGGTCAGCAGCTCGGTTGCGAGACGGCCTATACCGGCACGCCGGAATATGACGTCAACAAG
CAGATCGCCACTTTCGACCAGGCGCTGGCCCAGAACCCGGCCGGTATTCTCGTTCACCCGATGAACTCCGACCCGTTCAT
CGAGCCGATCAACCGGGCGATCGACCAGGGCACGGCGGTCGTGACCTTCGCGGCCGATGCGCCGCTTTCCAAGCGCATCT
CGTTCGTCACGTCGGACAATACCCGCGAAGGCACTTATGCGGCCGACGCGATTGCCGAAAAGATGGGTGGCAAGGGTGAG
TATGCCGTTCTCGAAAATCCGGGCCAGGACAACCACGACAAGCGTATCGCCGCCTTCATCGCCCGTATGGAGGAAAAATA
TCCTGATATGAAGCTGGTCGGCCGCGCTGCGTCCAACCAGGACCCGAACAAAGCCTATCAGGGTCTGATGAGCTTGGTGC
AGGCACATCCGAACATTGGCGCCGTCTTCATGCCGGAAGCGAATTCGGCCATCGGCGCTTCGCAGGCCAATAAGGAAAGC
GGCGGCAAGATCCTCGTGATGTGCGCCGACGTCAACGCCAACATTCTCGACATGATCAAGGCGGGCGAAGTGTTCGGCTC
GATCAACCCGAACCAGGGCATGCAGGGTTACATGGGCTTCATGCTCCTGTGGCTTGCCAAGCATCCGGAACTCATCGACC
CGATGAACGACGCCAAGCGCGTCGGCTTCAATCCGATGAGCATTCCGTTCGTCGACAACGGCTTGTCGATCGTCACGGCT
GCCAATGCCGACGATTTCTACTGGGACAAGTATCTGAAGCGGCGGGGCACCAAAGGCATCGAGGAGTAA

Upstream 100 bases:

>100_bases
TACCGGACTTCGAGGAGGAACTCCGGTCTTCAATGGATCATCGGCAGCCGCTGAGGCGCCGCCGGATATTTGGGAGGGGC
TTCGGCCTCGAGGAGGAAAC

Downstream 100 bases:

>100_bases
GCCTTTGTGCCGGCTGTCCGGGGGCAGGAAACTCCGGACAGCCACCGCGAGCGGGAGGAGGAGACGATGATGGCGTCGGT
GCCGGTTTTGGAAATCCGCA

Product: putative transporter component

Products: ADP; phosphate; ribose [Cytoplasm]; D-allose [Cytoplasm] [C]

Alternate protein names: ALBP [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRKFLSTTALAVLTSTLFAGAASADAENPFRCKPGEKYVMNVMVSGVEYWFPVYEMFKQAGQQLGCETAYTGTPEYDVNK
QIATFDQALAQNPAGILVHPMNSDPFIEPINRAIDQGTAVVTFAADAPLSKRISFVTSDNTREGTYAADAIAEKMGGKGE
YAVLENPGQDNHDKRIAAFIARMEEKYPDMKLVGRAASNQDPNKAYQGLMSLVQAHPNIGAVFMPEANSAIGASQANKES
GGKILVMCADVNANILDMIKAGEVFGSINPNQGMQGYMGFMLLWLAKHPELIDPMNDAKRVGFNPMSIPFVDNGLSIVTA
ANADDFYWDKYLKRRGTKGIEE

Sequences:

>Translated_342_residues
MRKFLSTTALAVLTSTLFAGAASADAENPFRCKPGEKYVMNVMVSGVEYWFPVYEMFKQAGQQLGCETAYTGTPEYDVNK
QIATFDQALAQNPAGILVHPMNSDPFIEPINRAIDQGTAVVTFAADAPLSKRISFVTSDNTREGTYAADAIAEKMGGKGE
YAVLENPGQDNHDKRIAAFIARMEEKYPDMKLVGRAASNQDPNKAYQGLMSLVQAHPNIGAVFMPEANSAIGASQANKES
GGKILVMCADVNANILDMIKAGEVFGSINPNQGMQGYMGFMLLWLAKHPELIDPMNDAKRVGFNPMSIPFVDNGLSIVTA
ANADDFYWDKYLKRRGTKGIEE
>Mature_342_residues
MRKFLSTTALAVLTSTLFAGAASADAENPFRCKPGEKYVMNVMVSGVEYWFPVYEMFKQAGQQLGCETAYTGTPEYDVNK
QIATFDQALAQNPAGILVHPMNSDPFIEPINRAIDQGTAVVTFAADAPLSKRISFVTSDNTREGTYAADAIAEKMGGKGE
YAVLENPGQDNHDKRIAAFIARMEEKYPDMKLVGRAASNQDPNKAYQGLMSLVQAHPNIGAVFMPEANSAIGASQANKES
GGKILVMCADVNANILDMIKAGEVFGSINPNQGMQGYMGFMLLWLAKHPELIDPMNDAKRVGFNPMSIPFVDNGLSIVTA
ANADDFYWDKYLKRRGTKGIEE

Specific function: Part of the binding-protein-dependent transport system AlsBAC for D-allose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790526, Length=266, Percent_Identity=25.9398496240602, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37223; Mature: 37223

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFLSTTALAVLTSTLFAGAASADAENPFRCKPGEKYVMNVMVSGVEYWFPVYEMFKQA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GQQLGCETAYTGTPEYDVNKQIATFDQALAQNPAGILVHPMNSDPFIEPINRAIDQGTAV
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEE
VTFAADAPLSKRISFVTSDNTREGTYAADAIAEKMGGKGEYAVLENPGQDNHDKRIAAFI
EEEECCCCHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
ARMEEKYPDMKLVGRAASNQDPNKAYQGLMSLVQAHPNIGAVFMPEANSAIGASQANKES
HHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCC
GGKILVMCADVNANILDMIKAGEVFGSINPNQGMQGYMGFMLLWLAKHPELIDPMNDAKR
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
VGFNPMSIPFVDNGLSIVTAANADDFYWDKYLKRRGTKGIEE
CCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRKFLSTTALAVLTSTLFAGAASADAENPFRCKPGEKYVMNVMVSGVEYWFPVYEMFKQA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GQQLGCETAYTGTPEYDVNKQIATFDQALAQNPAGILVHPMNSDPFIEPINRAIDQGTAV
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEE
VTFAADAPLSKRISFVTSDNTREGTYAADAIAEKMGGKGEYAVLENPGQDNHDKRIAAFI
EEEECCCCHHHCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
ARMEEKYPDMKLVGRAASNQDPNKAYQGLMSLVQAHPNIGAVFMPEANSAIGASQANKES
HHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCC
GGKILVMCADVNANILDMIKAGEVFGSINPNQGMQGYMGFMLLWLAKHPELIDPMNDAKR
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
VGFNPMSIPFVDNGLSIVTAANADDFYWDKYLKRRGTKGIEE
CCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; ribose [Periplasm]; D-allose [Periplasm]; ATP [C]

Specific reaction: ATP + H2O + ribose [Periplasm] = ADP + phosphate + ribose [Cytoplasm] D-allose [Periplasm] + ATP + H2O = D-allose [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8576032; 9401019; 10064713 [H]